Q9Z0J4 (NOS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitric oxide synthase, brain
EC=1.14.13.39

Alternative name(s):
Constitutive NOS
NC-NOS
NOS type I
Neuronal NOS
Short name=N-NOS
Short name=nNOS
Peptidyl-cysteine S-nitrosylase NOS1
bNOS

Gene names

Name:

Nos1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1429 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex. Ref.8
Catalytic activity	L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP By similarity.
Subunit structure	Homodimer. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location By similarity. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Interacts with HTR4. Forms a ternary complex with CAPON and RASD1. Interacts with VAC14 By similarity. Interacts with SLC6A4. Ref.5 Ref.6 Ref.7 Ref.9
Subcellular location	Cell membrane › sarcolemma; Peripheral membrane protein. Cell projection › dendritic spine By similarity. Note: In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines By similarity.
Tissue specificity	Widely expressed in the nervous system: expressed in cerebrum, olfactory bulb, hippocampus, midbrain, cerebellum, pons, medulla oblongata, and spinal cord. Also found in skeletal muscle, where it is localized beneath the sarcolemma of fast twitch muscle fibers, and in spleen, heart, kidney, and liver. N-NOS-1 and N-NOS-2 are found in all parts of the nervous system. NNOS beta and gamma occur in a region-specific manner in the brain and NNOS beta expression is developmentally regulated. NNOS Mu is only found in mature skeletal and cardiac muscles.
Induction	By cholinergic agonists acting at inositol phosphate-linked muscarinic receptors in cardiac myocytes.
Domain	The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14 By similarity.
Post-translational modification	Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro) By similarity.
Involvement in disease	Note=In MDX mice (mouse model of dystrophinopathy) the dystrophin complex is disrupted and nNOS is displaced from sarcolemma and accumulates in the cytosol.
Sequence similarities	Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Membrane
Coding sequence diversity	Alternative splicing
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to growth factor stimulus Inferred from mutant phenotype. Source: BHF-UCL exogenous drug catabolic process Inferred from mutant phenotype. Source: MGI negative regulation of calcium ion transport Inferred from mutant phenotype. Source: MGI negative regulation of hydrolase activity Inferred from mutant phenotype. Source: MGI negative regulation of potassium ion transport Inferred from mutant phenotype. Source: MGI negative regulation of serotonin uptake Inferred from direct assay Ref.9. Source: UniProtKB nitric oxide biosynthetic process Inferred from direct assay. Source: BHF-UCL peptidyl-cysteine S-nitrosylation Inferred from direct assay Ref.8. Source: MGI positive regulation of histone acetylation Inferred from mutant phenotype. Source: BHF-UCL positive regulation of transcription, DNA-dependent Inferred from mutant phenotype. Source: BHF-UCL regulation of sodium ion transport Inferred from mutant phenotype. Source: MGI striated muscle contraction Inferred from mutant phenotype. Source: MGI
Cellular component	cytoskeleton Inferred from direct assay. Source: BHF-UCL dendritic spine Inferred from electronic annotation. Source: UniProtKB-SubCell sarcolemma Inferred from direct assay. Source: BHF-UCL synapse Inferred from direct assay. Source: MGI
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from direct assay. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform N-NOS-1 (identifier: Q9Z0J4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform N-NOS-2 (identifier: Q9Z0J4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     504-608: Missing.

Isoform NNOS beta (identifier: Q9Z0J4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-230: Missing.
     231-236: TGIQVD → MRGLGS

Isoform NNOS gamma (identifier: Q9Z0J4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-331: Missing.

Isoform NNOS Mu (identifier: Q9Z0J4-5)

Also known as: Muscle-specific;

The sequence of this isoform differs from the canonical sequence as follows:
     839-839: K → KYPEPLRFFPRKGPSLSHVDSEAHSLVAARDSQHR

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1429

1429

Nitric oxide synthase, brain

PRO_0000170922

Regions

Domain

17 – 99

83

PDZ

Domain

755 – 935

181

Flavodoxin-like

Domain

990 – 1237

248

FAD-binding FR-type

Nucleotide binding

881 – 912

32

FMN By similarity

Nucleotide binding

1027 – 1038

12

FAD By similarity

Nucleotide binding

1170 – 1180

11

FAD By similarity

Nucleotide binding

1245 – 1263

19

NADP By similarity

Nucleotide binding

1343 – 1358

16

NADP By similarity

Region

1 – 200

200

Interaction with NOSIP By similarity

Region

163 – 240

78

PIN (nNOS-inhibiting protein) binding By similarity

Region

725 – 745

21

Calmodulin-binding Potential

Region

750 – 769

20

Tetrahydrobiopterin-binding By similarity

Sites

Metal binding

415

1

Iron (heme axial ligand) By similarity

Natural variations

Alternative sequence

1 – 331

331

Missing in isoform NNOS gamma.

VSP_003577

Alternative sequence

1 – 230

230

Missing in isoform NNOS beta.

VSP_003575

Alternative sequence

231 – 236

6

TGIQVD → MRGLGS in isoform NNOS beta.

VSP_003576

Alternative sequence

504 – 608

105

Missing in isoform N-NOS-2.

VSP_003578

Alternative sequence

839

1

K → KYPEPLRFFPRKGPSLSHVD SEAHSLVAARDSQHR in isoform NNOS Mu.

VSP_003579

Experimental info

Sequence conflict

1320

1

K → Q in BAE24878. Ref.3

Secondary structure

1

.

.

.

1429

Helix Strand Turn

Details...

Helix

731 – 744

14

Sequences

	Sequence		Length	Mass (Da)
	Isoform N-NOS-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3782848D65B41BFC	FASTA	1,429	160,472
	10 20 30 40 50 60 MEEHTFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG AAEQSGLIQA 70 80 90 100 110 120 GDIILAVNDR PLVDLSYDSA LEVLRGIASE THVVLILRGP EGFTTHLETT FTGDGTPKTI 130 140 150 160 170 180 RVTQPLGTPT KAVDLSRQPS ASKDQPLAVD RVPGPSNGPQ HAQGRGQGAG SVSQANGVAI 190 200 210 220 230 240 DPTMKNTKAN LQDSGEQDEL LKEIEPVLSI LTGGGKAVNR GGPAKAEMKD TGIQVDRDLD 250 260 270 280 290 300 GKLHKAPPLG GENDRVFNDL WGKGNVPVVL NNPYSENEQS PASGKQSPTK NGSPSRCPRF 310 320 330 340 350 360 LKVKNWETDV VLTDTLHLKS TLETGCTEQI CMGSIMLPSH HIRKSEDVRT KDQLFPLAKE 370 380 390 400 410 420 FLDQYYSSIK RFGSKAHMDR LEEVNKEIES TSTYQLKDTE LIYGAKHAWR NASRCVGRIQ 430 440 450 460 470 480 WSKLQVFDAR DCTTAHGMFN YICNHVKYAT NKGNLRSAIT IFPQRTDGKH DFRVWNSQLI 490 500 510 520 530 540 RYAGYKQPDG STLGDPANVE FTEICIQQGW KPPRGRFDVL PLLLQANGND PELFQIPPEL 550 560 570 580 590 600 VLEVPIRHPK FDWFKDLGLK WYGLPAVSNM LLEIGGLEFS ACPFSGWYMG TEIGVRDYCD 610 620 630 640 650 660 NSRYNILEEV AKKMDLDMRK TSSLWKDQAL VEINIAVLYS FQSDKVTIVD HHSATESFIK 670 680 690 700 710 720 HMENEYRCRG GCPADWVWIV PPMSGSITPV FHQEMLNYRL TPSFEYQPDP WNTHVWKGTN 730 740 750 760 770 780 GTPTKRRAIG FKKLAEAVKF SAKLMGQAMA KRVKATILYA TETGKSQAYA KTLCEIFKHA 790 800 810 820 830 840 FDAKAMSMEE YDIVHLEHEA LVLVVTSTFG NGDPPENGEK FGCALMEMRH PNSVQEERKS 850 860 870 880 890 900 YKVRFNSVSS YSDSRKSSGD GPDLRDNFES TGPLANVRFS VFGLGSRAYP HFCAFGHAVD 910 920 930 940 950 960 TLLEELGGER ILKMREGDEL CGQEEAFRTW AKKVFKAACD VFCVGDDVNI EKANNSLISN 970 980 990 1000 1010 1020 DRSWKRNKFR LTYVAEAPEL TQGLSNVHKK RVSAARLLSR QNLQSPKSSR STIFVRLHTN 1030 1040 1050 1060 1070 1080 GNQELQYQPG DHLGVFPGNH EDLVNALIER LEDAPPANHV VKVEMLEERN TALGVISNWK 1090 1100 1110 1120 1130 1140 DESRLPPCTI FQAFKYYLDI TTPPTPLQLQ QFASLATNEK EKQRLLVLSK GLQEYEEWKW 1150 1160 1170 1180 1190 1200 GKNPTMVEVL EEFPSIQMPA TLLLTQLSLL QPRYYSISSS PDMYPDEVHL TVAIVSYHTR 1210 1220 1230 1240 1250 1260 DGEGPVHHGV CSSWLNRIQA DDVVPCFVRG APSFHLPRNP QVPCILVGPG TGIAPFRSFW 1270 1280 1290 1300 1310 1320 QQRQFDIQHK GMNPCPMVLV FGCRQSKIDH IYREETLQAK NKGVFRELYT AYSREPDRPK 1330 1340 1350 1360 1370 1380 KYVQDVLQEQ LAESVYRALK EQGGHIYVCG DVTMAADVLK AIQRIMTQQG KLSEEDAGVF 1390 1400 1410 1420 ISRLRDDNRY HEDIFGVTLR TYEVTNRLRS ESIAFIEESK KDTDEVFSS
	Isoform N-NOS-2 [UniParc]. Checksum: F8532C1457C09EA4	FASTA	1,324	148,435
	10 20 30 40 50 60 MEEHTFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG AAEQSGLIQA 70 80 90 100 110 120 GDIILAVNDR PLVDLSYDSA LEVLRGIASE THVVLILRGP EGFTTHLETT FTGDGTPKTI 130 140 150 160 170 180 RVTQPLGTPT KAVDLSRQPS ASKDQPLAVD RVPGPSNGPQ HAQGRGQGAG SVSQANGVAI 190 200 210 220 230 240 DPTMKNTKAN LQDSGEQDEL LKEIEPVLSI LTGGGKAVNR GGPAKAEMKD TGIQVDRDLD 250 260 270 280 290 300 GKLHKAPPLG GENDRVFNDL WGKGNVPVVL NNPYSENEQS PASGKQSPTK NGSPSRCPRF 310 320 330 340 350 360 LKVKNWETDV VLTDTLHLKS TLETGCTEQI CMGSIMLPSH HIRKSEDVRT KDQLFPLAKE 370 380 390 400 410 420 FLDQYYSSIK RFGSKAHMDR LEEVNKEIES TSTYQLKDTE LIYGAKHAWR NASRCVGRIQ 430 440 450 460 470 480 WSKLQVFDAR DCTTAHGMFN YICNHVKYAT NKGNLRSAIT IFPQRTDGKH DFRVWNSQLI 490 500 510 520 530 540 RYAGYKQPDG STLGDPANVE FTEEVAKKMD LDMRKTSSLW KDQALVEINI AVLYSFQSDK 550 560 570 580 590 600 VTIVDHHSAT ESFIKHMENE YRCRGGCPAD WVWIVPPMSG SITPVFHQEM LNYRLTPSFE 610 620 630 640 650 660 YQPDPWNTHV WKGTNGTPTK RRAIGFKKLA EAVKFSAKLM GQAMAKRVKA TILYATETGK 670 680 690 700 710 720 SQAYAKTLCE IFKHAFDAKA MSMEEYDIVH LEHEALVLVV TSTFGNGDPP ENGEKFGCAL 730 740 750 760 770 780 MEMRHPNSVQ EERKSYKVRF NSVSSYSDSR KSSGDGPDLR DNFESTGPLA NVRFSVFGLG 790 800 810 820 830 840 SRAYPHFCAF GHAVDTLLEE LGGERILKMR EGDELCGQEE AFRTWAKKVF KAACDVFCVG 850 860 870 880 890 900 DDVNIEKANN SLISNDRSWK RNKFRLTYVA EAPELTQGLS NVHKKRVSAA RLLSRQNLQS 910 920 930 940 950 960 PKSSRSTIFV RLHTNGNQEL QYQPGDHLGV FPGNHEDLVN ALIERLEDAP PANHVVKVEM 970 980 990 1000 1010 1020 LEERNTALGV ISNWKDESRL PPCTIFQAFK YYLDITTPPT PLQLQQFASL ATNEKEKQRL 1030 1040 1050 1060 1070 1080 LVLSKGLQEY EEWKWGKNPT MVEVLEEFPS IQMPATLLLT QLSLLQPRYY SISSSPDMYP 1090 1100 1110 1120 1130 1140 DEVHLTVAIV SYHTRDGEGP VHHGVCSSWL NRIQADDVVP CFVRGAPSFH LPRNPQVPCI 1150 1160 1170 1180 1190 1200 LVGPGTGIAP FRSFWQQRQF DIQHKGMNPC PMVLVFGCRQ SKIDHIYREE TLQAKNKGVF 1210 1220 1230 1240 1250 1260 RELYTAYSRE PDRPKKYVQD VLQEQLAESV YRALKEQGGH IYVCGDVTMA ADVLKAIQRI 1270 1280 1290 1300 1310 1320 MTQQGKLSEE DAGVFISRLR DDNRYHEDIF GVTLRTYEVT NRLRSESIAF IEESKKDTDE VFSS
	Isoform NNOS beta [UniParc]. Checksum: 4ACB46D2B44CFEEA	FASTA	1,199	136,243
	10 20 30 40 50 60 MRGLGSRDLD GKLHKAPPLG GENDRVFNDL WGKGNVPVVL NNPYSENEQS PASGKQSPTK 70 80 90 100 110 120 NGSPSRCPRF LKVKNWETDV VLTDTLHLKS TLETGCTEQI CMGSIMLPSH HIRKSEDVRT 130 140 150 160 170 180 KDQLFPLAKE FLDQYYSSIK RFGSKAHMDR LEEVNKEIES TSTYQLKDTE LIYGAKHAWR 190 200 210 220 230 240 NASRCVGRIQ WSKLQVFDAR DCTTAHGMFN YICNHVKYAT NKGNLRSAIT IFPQRTDGKH 250 260 270 280 290 300 DFRVWNSQLI RYAGYKQPDG STLGDPANVE FTEICIQQGW KPPRGRFDVL PLLLQANGND 310 320 330 340 350 360 PELFQIPPEL VLEVPIRHPK FDWFKDLGLK WYGLPAVSNM LLEIGGLEFS ACPFSGWYMG 370 380 390 400 410 420 TEIGVRDYCD NSRYNILEEV AKKMDLDMRK TSSLWKDQAL VEINIAVLYS FQSDKVTIVD 430 440 450 460 470 480 HHSATESFIK HMENEYRCRG GCPADWVWIV PPMSGSITPV FHQEMLNYRL TPSFEYQPDP 490 500 510 520 530 540 WNTHVWKGTN GTPTKRRAIG FKKLAEAVKF SAKLMGQAMA KRVKATILYA TETGKSQAYA 550 560 570 580 590 600 KTLCEIFKHA FDAKAMSMEE YDIVHLEHEA LVLVVTSTFG NGDPPENGEK FGCALMEMRH 610 620 630 640 650 660 PNSVQEERKS YKVRFNSVSS YSDSRKSSGD GPDLRDNFES TGPLANVRFS VFGLGSRAYP 670 680 690 700 710 720 HFCAFGHAVD TLLEELGGER ILKMREGDEL CGQEEAFRTW AKKVFKAACD VFCVGDDVNI 730 740 750 760 770 780 EKANNSLISN DRSWKRNKFR LTYVAEAPEL TQGLSNVHKK RVSAARLLSR QNLQSPKSSR 790 800 810 820 830 840 STIFVRLHTN GNQELQYQPG DHLGVFPGNH EDLVNALIER LEDAPPANHV VKVEMLEERN 850 860 870 880 890 900 TALGVISNWK DESRLPPCTI FQAFKYYLDI TTPPTPLQLQ QFASLATNEK EKQRLLVLSK 910 920 930 940 950 960 GLQEYEEWKW GKNPTMVEVL EEFPSIQMPA TLLLTQLSLL QPRYYSISSS PDMYPDEVHL 970 980 990 1000 1010 1020 TVAIVSYHTR DGEGPVHHGV CSSWLNRIQA DDVVPCFVRG APSFHLPRNP QVPCILVGPG 1030 1040 1050 1060 1070 1080 TGIAPFRSFW QQRQFDIQHK GMNPCPMVLV FGCRQSKIDH IYREETLQAK NKGVFRELYT 1090 1100 1110 1120 1130 1140 AYSREPDRPK KYVQDVLQEQ LAESVYRALK EQGGHIYVCG DVTMAADVLK AIQRIMTQQG 1150 1160 1170 1180 1190 KLSEEDAGVF ISRLRDDNRY HEDIFGVTLR TYEVTNRLRS ESIAFIEESK KDTDEVFSS
	Isoform NNOS gamma [UniParc]. Checksum: 51C8FA95A3865266	FASTA	1,098	125,183
	10 20 30 40 50 60 MGSIMLPSHH IRKSEDVRTK DQLFPLAKEF LDQYYSSIKR FGSKAHMDRL EEVNKEIEST 70 80 90 100 110 120 STYQLKDTEL IYGAKHAWRN ASRCVGRIQW SKLQVFDARD CTTAHGMFNY ICNHVKYATN 130 140 150 160 170 180 KGNLRSAITI FPQRTDGKHD FRVWNSQLIR YAGYKQPDGS TLGDPANVEF TEICIQQGWK 190 200 210 220 230 240 PPRGRFDVLP LLLQANGNDP ELFQIPPELV LEVPIRHPKF DWFKDLGLKW YGLPAVSNML 250 260 270 280 290 300 LEIGGLEFSA CPFSGWYMGT EIGVRDYCDN SRYNILEEVA KKMDLDMRKT SSLWKDQALV 310 320 330 340 350 360 EINIAVLYSF QSDKVTIVDH HSATESFIKH MENEYRCRGG CPADWVWIVP PMSGSITPVF 370 380 390 400 410 420 HQEMLNYRLT PSFEYQPDPW NTHVWKGTNG TPTKRRAIGF KKLAEAVKFS AKLMGQAMAK 430 440 450 460 470 480 RVKATILYAT ETGKSQAYAK TLCEIFKHAF DAKAMSMEEY DIVHLEHEAL VLVVTSTFGN 490 500 510 520 530 540 GDPPENGEKF GCALMEMRHP NSVQEERKSY KVRFNSVSSY SDSRKSSGDG PDLRDNFEST 550 560 570 580 590 600 GPLANVRFSV FGLGSRAYPH FCAFGHAVDT LLEELGGERI LKMREGDELC GQEEAFRTWA 610 620 630 640 650 660 KKVFKAACDV FCVGDDVNIE KANNSLISND RSWKRNKFRL TYVAEAPELT QGLSNVHKKR 670 680 690 700 710 720 VSAARLLSRQ NLQSPKSSRS TIFVRLHTNG NQELQYQPGD HLGVFPGNHE DLVNALIERL 730 740 750 760 770 780 EDAPPANHVV KVEMLEERNT ALGVISNWKD ESRLPPCTIF QAFKYYLDIT TPPTPLQLQQ 790 800 810 820 830 840 FASLATNEKE KQRLLVLSKG LQEYEEWKWG KNPTMVEVLE EFPSIQMPAT LLLTQLSLLQ 850 860 870 880 890 900 PRYYSISSSP DMYPDEVHLT VAIVSYHTRD GEGPVHHGVC SSWLNRIQAD DVVPCFVRGA 910 920 930 940 950 960 PSFHLPRNPQ VPCILVGPGT GIAPFRSFWQ QRQFDIQHKG MNPCPMVLVF GCRQSKIDHI 970 980 990 1000 1010 1020 YREETLQAKN KGVFRELYTA YSREPDRPKK YVQDVLQEQL AESVYRALKE QGGHIYVCGD 1030 1040 1050 1060 1070 1080 VTMAADVLKA IQRIMTQQGK LSEEDAGVFI SRLRDDNRYH EDIFGVTLRT YEVTNRLRSE 1090 SIAFIEESKK DTDEVFSS
	Isoform NNOS Mu (Muscle-specific) [UniParc]. Checksum: 11689C595839D9A7	FASTA	1,463	164,343
	10 20 30 40 50 60 MEEHTFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG AAEQSGLIQA 70 80 90 100 110 120 GDIILAVNDR PLVDLSYDSA LEVLRGIASE THVVLILRGP EGFTTHLETT FTGDGTPKTI 130 140 150 160 170 180 RVTQPLGTPT KAVDLSRQPS ASKDQPLAVD RVPGPSNGPQ HAQGRGQGAG SVSQANGVAI 190 200 210 220 230 240 DPTMKNTKAN LQDSGEQDEL LKEIEPVLSI LTGGGKAVNR GGPAKAEMKD TGIQVDRDLD 250 260 270 280 290 300 GKLHKAPPLG GENDRVFNDL WGKGNVPVVL NNPYSENEQS PASGKQSPTK NGSPSRCPRF 310 320 330 340 350 360 LKVKNWETDV VLTDTLHLKS TLETGCTEQI CMGSIMLPSH HIRKSEDVRT KDQLFPLAKE 370 380 390 400 410 420 FLDQYYSSIK RFGSKAHMDR LEEVNKEIES TSTYQLKDTE LIYGAKHAWR NASRCVGRIQ 430 440 450 460 470 480 WSKLQVFDAR DCTTAHGMFN YICNHVKYAT NKGNLRSAIT IFPQRTDGKH DFRVWNSQLI 490 500 510 520 530 540 RYAGYKQPDG STLGDPANVE FTEICIQQGW KPPRGRFDVL PLLLQANGND PELFQIPPEL 550 560 570 580 590 600 VLEVPIRHPK FDWFKDLGLK WYGLPAVSNM LLEIGGLEFS ACPFSGWYMG TEIGVRDYCD 610 620 630 640 650 660 NSRYNILEEV AKKMDLDMRK TSSLWKDQAL VEINIAVLYS FQSDKVTIVD HHSATESFIK 670 680 690 700 710 720 HMENEYRCRG GCPADWVWIV PPMSGSITPV FHQEMLNYRL TPSFEYQPDP WNTHVWKGTN 730 740 750 760 770 780 GTPTKRRAIG FKKLAEAVKF SAKLMGQAMA KRVKATILYA TETGKSQAYA KTLCEIFKHA 790 800 810 820 830 840 FDAKAMSMEE YDIVHLEHEA LVLVVTSTFG NGDPPENGEK FGCALMEMRH PNSVQEERKY 850 860 870 880 890 900 PEPLRFFPRK GPSLSHVDSE AHSLVAARDS QHRSYKVRFN SVSSYSDSRK SSGDGPDLRD 910 920 930 940 950 960 NFESTGPLAN VRFSVFGLGS RAYPHFCAFG HAVDTLLEEL GGERILKMRE GDELCGQEEA 970 980 990 1000 1010 1020 FRTWAKKVFK AACDVFCVGD DVNIEKANNS LISNDRSWKR NKFRLTYVAE APELTQGLSN 1030 1040 1050 1060 1070 1080 VHKKRVSAAR LLSRQNLQSP KSSRSTIFVR LHTNGNQELQ YQPGDHLGVF PGNHEDLVNA 1090 1100 1110 1120 1130 1140 LIERLEDAPP ANHVVKVEML EERNTALGVI SNWKDESRLP PCTIFQAFKY YLDITTPPTP 1150 1160 1170 1180 1190 1200 LQLQQFASLA TNEKEKQRLL VLSKGLQEYE EWKWGKNPTM VEVLEEFPSI QMPATLLLTQ 1210 1220 1230 1240 1250 1260 LSLLQPRYYS ISSSPDMYPD EVHLTVAIVS YHTRDGEGPV HHGVCSSWLN RIQADDVVPC 1270 1280 1290 1300 1310 1320 FVRGAPSFHL PRNPQVPCIL VGPGTGIAPF RSFWQQRQFD IQHKGMNPCP MVLVFGCRQS 1330 1340 1350 1360 1370 1380 KIDHIYREET LQAKNKGVFR ELYTAYSREP DRPKKYVQDV LQEQLAESVY RALKEQGGHI 1390 1400 1410 1420 1430 1440 YVCGDVTMAA DVLKAIQRIM TQQGKLSEED AGVFISRLRD DNRYHEDIFG VTLRTYEVTN 1450 1460 RLRSESIAFI EESKKDTDEV FSS

References

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[1]	"Structural diversity of neuronal oxide synthase mRNA in the nervous system." Ogura T., Yokoyama T., Fujisawa H., Kurashima Y., Esumi H. Biochem. Biophys. Res. Commun. 193:1014-1022(1993) [PubMed: 7686743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS N-NOS-1 AND N-NOS-2). Strain: BALB/c. Tissue: Brain.
[2]	"Neuronal nitric-oxide synthase-mu, an alternatively spliced isoform expressed in differentiated skeletal muscle." Silvagno F., Xia H., Bredt D.S. J. Biol. Chem. 271:11204-11208(1996) [PubMed: 8626668] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NNOS MU). Tissue: Skeletal muscle.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1320-1429. Strain: C57BL/6J. Tissue: Spinal ganglion.
[4]	"Regulation of neuronal nitric oxide synthase through alternative transcripts." Brenman J.E., Xia H., Chao D.S., Black S.M., Bredt D.S. Dev. Neurosci. 19:224-231(1997) [PubMed: 9208206] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS NNOS BETA; NNOS GAMMA AND NNOS MU).
[5]	"Solution structure and backbone dynamics of the second PDZ domain of postsynaptic density-95." Tochio H., Hung F., Li M., Bredt D.S., Zhang M. J. Mol. Biol. 295:225-237(2000) [PubMed: 10623522] [Abstract] Cited for: INTERACTION WITH DLG4.
[6]	"Dexras1: a G protein specifically coupled to neuronal nitric oxide synthase via CAPON." Fang M., Jaffrey S.R., Sawa A., Ye K., Luo X., Snyder S.H. Neuron 28:183-193(2000) [PubMed: 11086993] [Abstract] Cited for: INTERACTION WITH RASD1 AND CAPON.
[7]	"New sorting nexin (SNX27) and NHERF specifically interact with the 5-HT4a receptor splice variant: roles in receptor targeting." Joubert L., Hanson B., Barthet G., Sebben M., Claeysen S., Hong W., Marin P., Dumuis A., Bockaert J. J. Cell Sci. 117:5367-5379(2004) [PubMed: 15466885] [Abstract] Cited for: INTERACTION WITH HTR4.
[8]	"Nitric oxide S-nitrosylates serine racemase, mediating feedback inhibition of D-serine formation." Mustafa A.K., Kumar M., Selvakumar B., Ho G.P., Ehmsen J.T., Barrow R.K., Amzel L.M., Snyder S.H. Proc. Natl. Acad. Sci. U.S.A. 104:2950-2955(2007) [PubMed: 17293453] [Abstract] Cited for: FUNCTION AS NITROSYLASE.
[9]	"Physical interaction between the serotonin transporter and neuronal nitric oxide synthase underlies reciprocal modulation of their activity." Chanrion B., Mannoury la Cour C., Bertaso F., Lerner-Natoli M., Freissmuth M., Millan M.J., Bockaert J., Marin P. Proc. Natl. Acad. Sci. U.S.A. 104:8119-8124(2007) [PubMed: 17452640] [Abstract] Cited for: INTERACTION WITH SLC6A4.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D14552 mRNA. Translation: BAA03415.1.
S81982 mRNA. Translation: AAB36469.1.
AK141904 mRNA. Translation: BAE24878.1.

IPI

IPI00129191.
IPI00229026.
IPI00229027.
IPI00229030.
IPI00309243.

PIR

JN0609.

RefSeq

NP_032738.1. NM_008712.2.

UniGene

Mm.442195.
Mm.44249.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O60	X-ray	1.55	B	725-747	[»]

ProteinModelPortal

Q9Z0J4.

SMR

Q9Z0J4. Positions 12-126, 298-716, 750-1413.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31556N.

IntAct

Q9Z0J4. 3 interactions.

STRING

Q9Z0J4.

PTM databases

PhosphoSite

Q9Z0J4.

Proteomic databases

PRIDE

Q9Z0J4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000111935; ENSMUSP00000107566; ENSMUSG00000029361.
ENSMUST00000142742; ENSMUSP00000120421; ENSMUSG00000029361.
ENSMUST00000171055; ENSMUSP00000127432; ENSMUSG00000029361.

GeneID

18125.

KEGG

mmu:18125.

UCSC

uc008zfy.1. mouse.

Organism-specific databases

CTD

4842.

MGI

MGI:97360. Nos1.

Phylogenomic databases

GeneTree

ENSGT00600000084325.

HOVERGEN

HBG000159.

PhylomeDB

Q9Z0J4.

Gene expression databases

ArrayExpress

Q9Z0J4.

Bgee

Q9Z0J4.

CleanEx

MM_NOS1.

Genevestigator

Q9Z0J4.

GermOnline

ENSMUSG00000029361. Mus musculus.

Family and domain databases

InterPro

IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. Nitric-oxide_synthase.
IPR004030. NO_synthase_oxygenase_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR001478. PDZ/DHR/GLGF.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]

Gene3D

G3DSA:1.20.990.10. NADPH_Cyt_P450_Rdtase_dom3. 1 hit.
G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit.

KO

K13240.

PANTHER

PTHR19384:SF5. PTHR19384:SF5. 1 hit.

Pfam

PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]

PIRSF

PIRSF000333. NOS. 1 hit.

PRINTS

PR00369. FLAVODOXIN.
PR00371. FPNCR.

SMART

SM00228. PDZ. 1 hit.
[Graphical view]

SUPFAM

SSF56512. NO_synthase_oxygenase_reg. 1 hit.
SSF50156. PDZ. 1 hit.
SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.

PROSITE

PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

293344.

SOURCE

Search...

Entry information

Entry name

NOS1_MOUSE

Accession

Primary (citable) accession number: Q9Z0J4
Secondary accession number(s): Q3UR10, Q64208

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 16, 2011
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program