Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Reversion-inducing cysteine-rich protein with Kazal motifs

Gene

Reck

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • blood vessel maturation Source: MGI
  • embryo implantation Source: MGI
  • embryonic forelimb morphogenesis Source: MGI
  • extracellular matrix organization Source: MGI
  • negative regulation of cell migration Source: MGI
  • negative regulation of metalloendopeptidase activity Source: BHF-UCL
  • positive regulation of Notch signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Reversion-inducing cysteine-rich protein with Kazal motifs
Short name:
mRECK
Gene namesi
Name:Reck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1855698. Reck.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 942920Reversion-inducing cysteine-rich protein with Kazal motifsPRO_0000016585Add
BLAST
Propeptidei943 – 97129Removed in mature formSequence analysisPRO_0000016586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence analysis
Disulfide bondi633 ↔ 658PROSITE-ProRule annotation
Disulfide bondi635 ↔ 654PROSITE-ProRule annotation
Disulfide bondi643 ↔ 677PROSITE-ProRule annotation
Lipidationi942 – 9421GPI-anchor amidated serineSequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ9Z0J1.
PaxDbiQ9Z0J1.
PRIDEiQ9Z0J1.

PTM databases

iPTMnetiQ9Z0J1.
PhosphoSiteiQ9Z0J1.

Expressioni

Developmental stagei

In E10.5 embryos the RECK protein is widely expressed in mesenchymal tissues and is relatively abundant in the marginal zone of the neural tube and large blood vessels such as the aorta.

Gene expression databases

BgeeiENSMUSG00000028476.
GenevisibleiQ9Z0J1. MM.

Interactioni

Subunit structurei

Interacts with MMP-9.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030198.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0J1.
SMRiQ9Z0J1. Positions 630-657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 8448Knot 1Add
BLAST
Repeati104 – 14138Knot 2Add
BLAST
Repeati151 – 19747Knot 3Add
BLAST
Repeati216 – 26348Knot 4Add
BLAST
Repeati292 – 33847Knot 5Add
BLAST
Domaini627 – 67347Kazal-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini698 – 75255Kazal-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini704 – 75047Kazal-like 2; degeneratePROSITE-ProRule annotationAdd
BLAST
Domaini753 – 78937Kazal-like 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 3383025 X Knot repeatsAdd
BLAST

Sequence similaritiesi

Contains 3 Kazal-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00390000018540.
HOGENOMiHOG000007167.
HOVERGENiHBG036100.
InParanoidiQ9Z0J1.
KOiK17461.
OMAiVNRKGCP.
OrthoDBiEOG091G017H.
TreeFamiTF324424.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 3 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 3 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVRASPRS ALLLLLAAAG VAEVTGGLAP GSAGAVCCNH SKDNQMCRDV
60 70 80 90 100
CEQIFSSKSE SRLKHLLQRA PDYCPETMVE IWSCMNSSLP GVFKKSDGWV
110 120 130 140 150
GLGCCELAIG LECRQACKQA SSKNDISKVC RKEYENALFS CISRNEMGSV
160 170 180 190 200
CCSYAGHHTN CREFCQAIFR TDSSPGPSQI KAVENYCASI SPQLIHCVNN
210 220 230 240 250
YTQSYPMRNP TDSLYCCDRA EDHACQNACK RILMSKKTEM EIVDGLIEGC
260 270 280 290 300
KTQPLPQDPL WQCFLESSQS VHPGVTVHPP PSTGLDGAKL HCCSKANTST
310 320 330 340 350
CRELCTKLYS MSWGNTQSWQ EFDRICEYNP VEVSMLTCLA DVREPCQLGC
360 370 380 390 400
TNLTYCTNFN NRPTELFRSC TAQSDQGAMS DMKLWEKGSI KMPFISIPVL
410 420 430 440 450
DIKTCQPEMW KAVACSLQIK PCHSKSRGSI ICKSDCVEIL KKCGDQNKFP
460 470 480 490 500
EEHTAESICE FLSPADDLES CIPLDTYLRP SALGNIIEEV THPCNPNPCP
510 520 530 540 550
ANELCEVNRK GCPSADPCLP YSCVQGCKLG EASDFIVRQG TLIQVPSSAG
560 570 580 590 600
EVGCYKICSC GQSGLLENCM EMHCIDLQKS CIVGGKRKSH GTSFTIDCNV
610 620 630 640 650
CSCFAGNLVC STRLCLSEHS SDDDRRTFTG LPCNCADQFV PVCAQNGRTY
660 670 680 690 700
PSACIARCVG LQDHQFEFGP CISKNPCNPN LCPKSQRCVP KPQVCLTTFD
710 720 730 740 750
KFGCSQYECV PRQLTCDQAR DPVCDTDHME HSNLCTLYQR GKSLSYRGPC
760 770 780 790 800
QPFCRAKEPV CGHNGETYSS VCAAYSDRVA VDYYGPCQAV GVLSEYSAVA
810 820 830 840 850
ECAAVKCPSL SAIGCKPIIP PGACCPLCAG MLRVLFDKEK LDTIAKVTSK
860 870 880 890 900
KPITVVEILQ KVRMHVSVPQ CDVFGYLSIE SEIVILIIPV DHYPKALQIE
910 920 930 940 950
ACNKEAEKIE SLINSDSPTL ASHVPLSALI ISQVQVSSSL PSSAVVGRPL
960 970
FHSLLLLLSL GLTVHLLWTR P
Length:971
Mass (Da):106,082
Last modified:July 27, 2011 - v2
Checksum:iCD5C462EA08A6866
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti447 – 4493NKF → PKG in BAA34061 (PubMed:9789069).Curated
Sequence conflicti539 – 5391Q → P in BAA34061 (PubMed:9789069).Curated
Sequence conflicti662 – 6632QD → HH in BAA34061 (PubMed:9789069).Curated
Sequence conflicti757 – 7571K → T in BAA34061 (PubMed:9789069).Curated
Sequence conflicti804 – 8041A → S in BAA34061 (PubMed:9789069).Curated
Sequence conflicti921 – 9211A → E in BAA34061 (PubMed:9789069).Curated
Sequence conflicti925 – 9251P → H in BAA34061 (PubMed:9789069).Curated
Sequence conflicti960 – 9601L → W in BAA34061 (PubMed:9789069).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006960 mRNA. Translation: BAA34061.1.
AL772204, AL773539 Genomic DNA. Translation: CAM22508.1.
AL773539, AL772204 Genomic DNA. Translation: CAM27572.1.
CCDSiCCDS18116.1.
PIRiPC7035.
RefSeqiNP_057887.2. NM_016678.2.
UniGeneiMm.331573.

Genome annotation databases

EnsembliENSMUST00000030198; ENSMUSP00000030198; ENSMUSG00000028476.
GeneIDi53614.
KEGGimmu:53614.
UCSCiuc012ddg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006960 mRNA. Translation: BAA34061.1.
AL772204, AL773539 Genomic DNA. Translation: CAM22508.1.
AL773539, AL772204 Genomic DNA. Translation: CAM27572.1.
CCDSiCCDS18116.1.
PIRiPC7035.
RefSeqiNP_057887.2. NM_016678.2.
UniGeneiMm.331573.

3D structure databases

ProteinModelPortaliQ9Z0J1.
SMRiQ9Z0J1. Positions 630-657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030198.

PTM databases

iPTMnetiQ9Z0J1.
PhosphoSiteiQ9Z0J1.

Proteomic databases

MaxQBiQ9Z0J1.
PaxDbiQ9Z0J1.
PRIDEiQ9Z0J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030198; ENSMUSP00000030198; ENSMUSG00000028476.
GeneIDi53614.
KEGGimmu:53614.
UCSCiuc012ddg.1. mouse.

Organism-specific databases

CTDi8434.
MGIiMGI:1855698. Reck.

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00390000018540.
HOGENOMiHOG000007167.
HOVERGENiHBG036100.
InParanoidiQ9Z0J1.
KOiK17461.
OMAiVNRKGCP.
OrthoDBiEOG091G017H.
TreeFamiTF324424.

Miscellaneous databases

PROiQ9Z0J1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028476.
GenevisibleiQ9Z0J1. MM.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 3 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 3 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECK_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0J1
Secondary accession number(s): B1AWM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.