Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Reversion-inducing cysteine-rich protein with Kazal motifs

Gene

Reck

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • blood vessel maturation Source: MGI
  • embryo implantation Source: MGI
  • embryonic forelimb morphogenesis Source: MGI
  • extracellular matrix organization Source: MGI
  • negative regulation of cell migration Source: MGI
  • negative regulation of metalloendopeptidase activity Source: BHF-UCL
  • positive regulation of Notch signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Reversion-inducing cysteine-rich protein with Kazal motifs
Short name:
mRECK
Gene namesi
Name:Reck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1855698. Reck.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001658523 – 942Reversion-inducing cysteine-rich protein with Kazal motifsAdd BLAST920
PropeptideiPRO_0000016586943 – 971Removed in mature formSequence analysisAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...)Sequence analysis1
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi352N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi633 ↔ 658PROSITE-ProRule annotation
Disulfide bondi635 ↔ 654PROSITE-ProRule annotation
Disulfide bondi643 ↔ 677PROSITE-ProRule annotation
Lipidationi942GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ9Z0J1.
PaxDbiQ9Z0J1.
PRIDEiQ9Z0J1.

PTM databases

iPTMnetiQ9Z0J1.
PhosphoSitePlusiQ9Z0J1.

Expressioni

Developmental stagei

In E10.5 embryos the RECK protein is widely expressed in mesenchymal tissues and is relatively abundant in the marginal zone of the neural tube and large blood vessels such as the aorta.

Gene expression databases

BgeeiENSMUSG00000028476.
GenevisibleiQ9Z0J1. MM.

Interactioni

Subunit structurei

Interacts with MMP-9.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030198.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0J1.
SMRiQ9Z0J1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 84Knot 1Add BLAST48
Repeati104 – 141Knot 2Add BLAST38
Repeati151 – 197Knot 3Add BLAST47
Repeati216 – 263Knot 4Add BLAST48
Repeati292 – 338Knot 5Add BLAST47
Domaini627 – 673Kazal-like 1PROSITE-ProRule annotationAdd BLAST47
Domaini698 – 752Kazal-like 2PROSITE-ProRule annotationAdd BLAST55
Domaini704 – 750Kazal-like 2; degeneratePROSITE-ProRule annotationAdd BLAST47
Domaini753 – 789Kazal-like 3PROSITE-ProRule annotationAdd BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 3385 X Knot repeatsAdd BLAST302

Sequence similaritiesi

Contains 3 Kazal-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00390000018540.
HOGENOMiHOG000007167.
HOVERGENiHBG036100.
InParanoidiQ9Z0J1.
KOiK17461.
OMAiVNRKGCP.
OrthoDBiEOG091G017H.
TreeFamiTF324424.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 3 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 3 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVRASPRS ALLLLLAAAG VAEVTGGLAP GSAGAVCCNH SKDNQMCRDV
60 70 80 90 100
CEQIFSSKSE SRLKHLLQRA PDYCPETMVE IWSCMNSSLP GVFKKSDGWV
110 120 130 140 150
GLGCCELAIG LECRQACKQA SSKNDISKVC RKEYENALFS CISRNEMGSV
160 170 180 190 200
CCSYAGHHTN CREFCQAIFR TDSSPGPSQI KAVENYCASI SPQLIHCVNN
210 220 230 240 250
YTQSYPMRNP TDSLYCCDRA EDHACQNACK RILMSKKTEM EIVDGLIEGC
260 270 280 290 300
KTQPLPQDPL WQCFLESSQS VHPGVTVHPP PSTGLDGAKL HCCSKANTST
310 320 330 340 350
CRELCTKLYS MSWGNTQSWQ EFDRICEYNP VEVSMLTCLA DVREPCQLGC
360 370 380 390 400
TNLTYCTNFN NRPTELFRSC TAQSDQGAMS DMKLWEKGSI KMPFISIPVL
410 420 430 440 450
DIKTCQPEMW KAVACSLQIK PCHSKSRGSI ICKSDCVEIL KKCGDQNKFP
460 470 480 490 500
EEHTAESICE FLSPADDLES CIPLDTYLRP SALGNIIEEV THPCNPNPCP
510 520 530 540 550
ANELCEVNRK GCPSADPCLP YSCVQGCKLG EASDFIVRQG TLIQVPSSAG
560 570 580 590 600
EVGCYKICSC GQSGLLENCM EMHCIDLQKS CIVGGKRKSH GTSFTIDCNV
610 620 630 640 650
CSCFAGNLVC STRLCLSEHS SDDDRRTFTG LPCNCADQFV PVCAQNGRTY
660 670 680 690 700
PSACIARCVG LQDHQFEFGP CISKNPCNPN LCPKSQRCVP KPQVCLTTFD
710 720 730 740 750
KFGCSQYECV PRQLTCDQAR DPVCDTDHME HSNLCTLYQR GKSLSYRGPC
760 770 780 790 800
QPFCRAKEPV CGHNGETYSS VCAAYSDRVA VDYYGPCQAV GVLSEYSAVA
810 820 830 840 850
ECAAVKCPSL SAIGCKPIIP PGACCPLCAG MLRVLFDKEK LDTIAKVTSK
860 870 880 890 900
KPITVVEILQ KVRMHVSVPQ CDVFGYLSIE SEIVILIIPV DHYPKALQIE
910 920 930 940 950
ACNKEAEKIE SLINSDSPTL ASHVPLSALI ISQVQVSSSL PSSAVVGRPL
960 970
FHSLLLLLSL GLTVHLLWTR P
Length:971
Mass (Da):106,082
Last modified:July 27, 2011 - v2
Checksum:iCD5C462EA08A6866
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti447 – 449NKF → PKG in BAA34061 (PubMed:9789069).Curated3
Sequence conflicti539Q → P in BAA34061 (PubMed:9789069).Curated1
Sequence conflicti662 – 663QD → HH in BAA34061 (PubMed:9789069).Curated2
Sequence conflicti757K → T in BAA34061 (PubMed:9789069).Curated1
Sequence conflicti804A → S in BAA34061 (PubMed:9789069).Curated1
Sequence conflicti921A → E in BAA34061 (PubMed:9789069).Curated1
Sequence conflicti925P → H in BAA34061 (PubMed:9789069).Curated1
Sequence conflicti960L → W in BAA34061 (PubMed:9789069).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006960 mRNA. Translation: BAA34061.1.
AL772204, AL773539 Genomic DNA. Translation: CAM22508.1.
AL773539, AL772204 Genomic DNA. Translation: CAM27572.1.
CCDSiCCDS18116.1.
PIRiPC7035.
RefSeqiNP_057887.2. NM_016678.2.
UniGeneiMm.331573.

Genome annotation databases

EnsembliENSMUST00000030198; ENSMUSP00000030198; ENSMUSG00000028476.
GeneIDi53614.
KEGGimmu:53614.
UCSCiuc012ddg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006960 mRNA. Translation: BAA34061.1.
AL772204, AL773539 Genomic DNA. Translation: CAM22508.1.
AL773539, AL772204 Genomic DNA. Translation: CAM27572.1.
CCDSiCCDS18116.1.
PIRiPC7035.
RefSeqiNP_057887.2. NM_016678.2.
UniGeneiMm.331573.

3D structure databases

ProteinModelPortaliQ9Z0J1.
SMRiQ9Z0J1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030198.

PTM databases

iPTMnetiQ9Z0J1.
PhosphoSitePlusiQ9Z0J1.

Proteomic databases

MaxQBiQ9Z0J1.
PaxDbiQ9Z0J1.
PRIDEiQ9Z0J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030198; ENSMUSP00000030198; ENSMUSG00000028476.
GeneIDi53614.
KEGGimmu:53614.
UCSCiuc012ddg.1. mouse.

Organism-specific databases

CTDi8434.
MGIiMGI:1855698. Reck.

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00390000018540.
HOGENOMiHOG000007167.
HOVERGENiHBG036100.
InParanoidiQ9Z0J1.
KOiK17461.
OMAiVNRKGCP.
OrthoDBiEOG091G017H.
TreeFamiTF324424.

Miscellaneous databases

PROiQ9Z0J1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028476.
GenevisibleiQ9Z0J1. MM.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 3 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 3 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECK_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0J1
Secondary accession number(s): B1AWM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.