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Protein

CAP-Gly domain-containing linker protein 2

Gene

Clip2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Seems to link microtubules to dendritic lamellar body (DLB), a membranous organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. May operate in the control of brain-specific organelle translocations (By similarity).By similarity

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • microtubule plus-end binding Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
CAP-Gly domain-containing linker protein 2
Alternative name(s):
Cytoplasmic linker protein 115
Short name:
CLIP-115
Cytoplasmic linker protein 2
Gene namesi
Name:Clip2
Synonyms:Cyln2, Kiaa0291
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1313136. Clip2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • microtubule plus-end Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835161 – 1047CAP-Gly domain-containing linker protein 2Add BLAST1047

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineCombined sources1
Modified residuei203PhosphoserineBy similarity1
Modified residuei208PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei315PhosphoserineCombined sources1
Modified residuei924PhosphoserineBy similarity1
Modified residuei974PhosphoserineCombined sources1
Modified residuei980PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z0H8.
PeptideAtlasiQ9Z0H8.
PRIDEiQ9Z0H8.

PTM databases

iPTMnetiQ9Z0H8.
PhosphoSitePlusiQ9Z0H8.

Expressioni

Tissue specificityi

Expressed in the brain, and very low levels in kidneys.1 Publication

Developmental stagei

Expressed at E10.5, expression declines until birth after which it suddenly increases. Expression gradually decreases until postnatal day 10, (the day when DLBs start to occur), then again increases and reaches the levels present in adult brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000063146.
CleanExiMM_CLIP2.
GenevisibleiQ9Z0H8. MM.

Interactioni

Subunit structurei

Interacts with CLASP1 and CLASP2 (PubMed:11290329). Binds preferentially to tyrosinated microtubules, and only marginally to detyrosinated microtubules (PubMed:16954346).2 Publications

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • microtubule plus-end binding Source: MGI

Protein-protein interaction databases

BioGridi234703. 2 interactors.
IntActiQ9Z0H8. 6 interactors.
MINTiMINT-4128805.
STRINGi10090.ENSMUSP00000098212.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0H8.
SMRiQ9Z0H8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 142CAP-Gly 1PROSITE-ProRule annotationAdd BLAST43
Domaini240 – 282CAP-Gly 2PROSITE-ProRule annotationAdd BLAST43

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili355 – 525Sequence analysisAdd BLAST171
Coiled coili564 – 637Sequence analysisAdd BLAST74
Coiled coili677 – 1017Sequence analysisAdd BLAST341

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi315 – 346Ser-richAdd BLAST32

Sequence similaritiesi

Contains 2 CAP-Gly domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG4568. Eukaryota.
COG5244. LUCA.
GeneTreeiENSGT00760000119173.
HOGENOMiHOG000092755.
HOVERGENiHBG007123.
InParanoidiQ9Z0H8.
KOiK10422.
OMAiHMIESSD.
OrthoDBiEOG091G0J1Y.
PhylomeDBiQ9Z0H8.
TreeFamiTF326096.

Family and domain databases

Gene3Di2.30.30.190. 2 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR028394. CLIP2.
[Graphical view]
PANTHERiPTHR18916:SF10. PTHR18916:SF10. 1 hit.
PfamiPF01302. CAP_GLY. 2 hits.
[Graphical view]
SMARTiSM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 2 hits.
PROSITEiPS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z0H8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQKPSGLKPP GRGGKHSSPV GRPSVGSASS SVVASTSGSK EGSPLHKQAS
60 70 80 90 100
GPSSSGAATT VSEKPGPKAA EVGDDFLGDF VVGERVWVNG VKPGVVQYLG
110 120 130 140 150
ETQFAPGQWA GVVLDDPVGK NDGAVGGVRY FECPALQGIF TRPSKLTRQP
160 170 180 190 200
TAEGSGSDTH SVESLTAQNL SLHSGTATPP LTGRVIPLRE SVLNSSVKTG
210 220 230 240 250
NESGSNLSDS GSVKRGDKDL HLGDRVLVGG TKTGVVRYVG ETDFAKGEWC
260 270 280 290 300
GVELDEPLGK NDGAVAGTRY FQCPPKFGLF APIHKVIRIG FPSTSPAKAK
310 320 330 340 350
KTKRMAMGVS ALTHSPSSSS ISSVSSVASS VGGRPSRSGL LTETSSRYAR
360 370 380 390 400
KISGTTALQE ALKEKQQHIE QLLAERDLER AEVAKATSHI CEVEKEIALL
410 420 430 440 450
KAQHEQYVAE AEEKLQRARL LVENVRKEKV DLSNQLEEER RKVEDLQFRV
460 470 480 490 500
EEESITKGDL ETQTQLEHAR IGELEQSLLL EKAQAERLLR ELADNRLTTV
510 520 530 540 550
AEKSRVLQLE EELSLRRGEI EELQHCLLQS GPPPADHPEA AETLRLRERL
560 570 580 590 600
LSASKEHQRD STLLQDKYEH MLKTYQTEVD KLRAANEKYA QEVADLKAKV
610 620 630 640 650
QQATTENMGL MDNWKSKLDS LASDHQKSLE DLKATLNSGP GAQQKEIGEL
660 670 680 690 700
KALVEGIKME HQLELGNLQA KHDLETAMHG KEKEGLRQKL QEVQEELAGL
710 720 730 740 750
QQHWREQLEE QASQHRLELQ EAQDQCRDAQ LRAQELEGLD VEYRGQAQAI
760 770 780 790 800
EFLKEQISLA EKKMLDYEML QRAEAQSRQE AERLREKLLV AENRLQAAES
810 820 830 840 850
LCSAQHSHVI ESSDLSEETI RMKETVEGLQ DKLNKRDKEV TALTSQMDML
860 870 880 890 900
RAQVSALENK CKSGEKKIDS LLKEKRRLEA ELEAVSRKTH DASGQLVHIS
910 920 930 940 950
QELLRKERSL NELRVLLLEA NRHSPGPERD LSREVHKAEW RIKEQKLKDD
960 970 980 990 1000
IRGLREKLTG LDKEKSLSEQ RRYSLIDPAS PPELLKLQHQ LVSTEDALRD
1010 1020 1030 1040
ALNQAQQVER LVEALRGCSD RTQTISNSGS ANGIHQPDKA HKQEDKH
Length:1,047
Mass (Da):115,910
Last modified:September 27, 2005 - v2
Checksum:i8F46948122F58872
GO
Isoform 2 (identifier: Q9Z0H8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     462-496: Missing.

Show »
Length:1,012
Mass (Da):111,855
Checksum:i5CD0F481CFE7CFB0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25V → I in AAH39162 (PubMed:15489334).Curated1
Sequence conflicti59T → A in CAA13068 (PubMed:9799601).Curated1
Sequence conflicti59T → A in CAA13069 (PubMed:9799601).Curated1
Sequence conflicti79D → H in CAA13068 (PubMed:9799601).Curated1
Sequence conflicti79D → H in CAA13069 (PubMed:9799601).Curated1
Sequence conflicti127G → A in CAA13068 (PubMed:9799601).Curated1
Sequence conflicti127G → A in CAA13069 (PubMed:9799601).Curated1
Sequence conflicti334 – 335RP → PA in CAA13068 (PubMed:9799601).Curated2
Sequence conflicti334 – 335RP → PA in CAA13069 (PubMed:9799601).Curated2
Sequence conflicti356T → I in CAA13068 (PubMed:9799601).Curated1
Sequence conflicti356T → I in CAA13069 (PubMed:9799601).Curated1
Sequence conflicti559R → D in CAA13068 (PubMed:9799601).Curated1
Sequence conflicti559R → D in CAA13069 (PubMed:9799601).Curated1
Sequence conflicti713 – 719SQHRLEL → AASAEA in CAA13068 (PubMed:9799601).Curated7
Sequence conflicti733A → V in CAA13068 (PubMed:9799601).Curated1
Sequence conflicti733A → V in CAA13069 (PubMed:9799601).Curated1
Sequence conflicti856A → V in CAA13068 (PubMed:9799601).Curated1
Sequence conflicti856A → V in CAA13069 (PubMed:9799601).Curated1
Sequence conflicti1003N → D in AAH39162 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015683462 – 496Missing in isoform 2. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ228863 Genomic DNA. Translation: CAA13068.1.
AJ228865
, AJ228868, AJ228869, AJ228870, AJ228872, AJ228876, AJ228880, AJ228867, AJ228875, AJ228871, AJ228873, AJ228877, AJ228866, AJ228879, AJ228878, AJ228874 Genomic DNA. Translation: CAA13069.1.
AF289667 Genomic DNA. Translation: AAF99340.1.
AF289664 Genomic DNA. Translation: AAF99333.1.
BC039162 mRNA. Translation: AAH39162.1.
BC053048 mRNA. Translation: AAH53048.1.
CCDSiCCDS39309.1. [Q9Z0H8-2]
CCDS39310.1. [Q9Z0H8-1]
PIRiT42720.
RefSeqiNP_001034251.1. NM_001039162.2. [Q9Z0H8-2]
NP_034120.2. NM_009990.3. [Q9Z0H8-1]
UniGeneiMm.255138.

Genome annotation databases

EnsembliENSMUST00000036999; ENSMUSP00000037431; ENSMUSG00000063146. [Q9Z0H8-2]
ENSMUST00000100647; ENSMUSP00000098212; ENSMUSG00000063146. [Q9Z0H8-1]
GeneIDi269713.
KEGGimmu:269713.
UCSCiuc008zwi.2. mouse. [Q9Z0H8-1]
uc008zwj.2. mouse. [Q9Z0H8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ228863 Genomic DNA. Translation: CAA13068.1.
AJ228865
, AJ228868, AJ228869, AJ228870, AJ228872, AJ228876, AJ228880, AJ228867, AJ228875, AJ228871, AJ228873, AJ228877, AJ228866, AJ228879, AJ228878, AJ228874 Genomic DNA. Translation: CAA13069.1.
AF289667 Genomic DNA. Translation: AAF99340.1.
AF289664 Genomic DNA. Translation: AAF99333.1.
BC039162 mRNA. Translation: AAH39162.1.
BC053048 mRNA. Translation: AAH53048.1.
CCDSiCCDS39309.1. [Q9Z0H8-2]
CCDS39310.1. [Q9Z0H8-1]
PIRiT42720.
RefSeqiNP_001034251.1. NM_001039162.2. [Q9Z0H8-2]
NP_034120.2. NM_009990.3. [Q9Z0H8-1]
UniGeneiMm.255138.

3D structure databases

ProteinModelPortaliQ9Z0H8.
SMRiQ9Z0H8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234703. 2 interactors.
IntActiQ9Z0H8. 6 interactors.
MINTiMINT-4128805.
STRINGi10090.ENSMUSP00000098212.

PTM databases

iPTMnetiQ9Z0H8.
PhosphoSitePlusiQ9Z0H8.

Proteomic databases

PaxDbiQ9Z0H8.
PeptideAtlasiQ9Z0H8.
PRIDEiQ9Z0H8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036999; ENSMUSP00000037431; ENSMUSG00000063146. [Q9Z0H8-2]
ENSMUST00000100647; ENSMUSP00000098212; ENSMUSG00000063146. [Q9Z0H8-1]
GeneIDi269713.
KEGGimmu:269713.
UCSCiuc008zwi.2. mouse. [Q9Z0H8-1]
uc008zwj.2. mouse. [Q9Z0H8-2]

Organism-specific databases

CTDi7461.
MGIiMGI:1313136. Clip2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4568. Eukaryota.
COG5244. LUCA.
GeneTreeiENSGT00760000119173.
HOGENOMiHOG000092755.
HOVERGENiHBG007123.
InParanoidiQ9Z0H8.
KOiK10422.
OMAiHMIESSD.
OrthoDBiEOG091G0J1Y.
PhylomeDBiQ9Z0H8.
TreeFamiTF326096.

Miscellaneous databases

PROiQ9Z0H8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063146.
CleanExiMM_CLIP2.
GenevisibleiQ9Z0H8. MM.

Family and domain databases

Gene3Di2.30.30.190. 2 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR028394. CLIP2.
[Graphical view]
PANTHERiPTHR18916:SF10. PTHR18916:SF10. 1 hit.
PfamiPF01302. CAP_GLY. 2 hits.
[Graphical view]
SMARTiSM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 2 hits.
PROSITEiPS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLIP2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0H8
Secondary accession number(s): Q7TSI9, Q8CHU1, Q9EP81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.