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Q9Z0H8 (CLIP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CAP-Gly domain-containing linker protein 2
Alternative name(s):
Cytoplasmic linker protein 115
Short name=CLIP-115
Cytoplasmic linker protein 2
Gene names
Name:Clip2
Synonyms:Cyln2, Kiaa0291
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1047 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to link microtubules to dendritic lamellar body (DLB), a membranous organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. May operate in the control of brain-specific organelle translocations By similarity.

Subunit structure

Interacts with CLASP1 and CLASP2. Ref.4

Subcellular location

Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Note: Associated with the cytoskeleton By similarity.

Tissue specificity

Expressed in the brain, and very low levels in kidneys. Ref.5

Developmental stage

Expressed at E10.5, expression declines until birth after which it suddenly increases. Expression gradually decreases until postnatal day 10, (the day when DLBs start to occur), then again increases and reaches the levels present in adult brain. Ref.5

Sequence similarities

Contains 2 CAP-Gly domains.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasmic microtubule

Inferred from direct assay Ref.4. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Z0H8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Z0H8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     462-496: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10471047CAP-Gly domain-containing linker protein 2
PRO_0000083516

Regions

Domain100 – 14243CAP-Gly 1
Domain240 – 28243CAP-Gly 2
Coiled coil355 – 525171 Potential
Coiled coil564 – 63774 Potential
Coiled coil677 – 1017341 Potential
Compositional bias315 – 34632Ser-rich

Amino acid modifications

Modified residue501Phosphoserine Ref.6
Modified residue3531Phosphoserine Ref.7

Natural variations

Alternative sequence462 – 49635Missing in isoform 2.
VSP_015683

Experimental info

Sequence conflict251V → I in AAH39162. Ref.3
Sequence conflict591T → A in CAA13068. Ref.1
Sequence conflict591T → A in CAA13069. Ref.1
Sequence conflict791D → H in CAA13068. Ref.1
Sequence conflict791D → H in CAA13069. Ref.1
Sequence conflict1271G → A in CAA13068. Ref.1
Sequence conflict1271G → A in CAA13069. Ref.1
Sequence conflict334 – 3352RP → PA in CAA13068. Ref.1
Sequence conflict334 – 3352RP → PA in CAA13069. Ref.1
Sequence conflict3561T → I in CAA13068. Ref.1
Sequence conflict3561T → I in CAA13069. Ref.1
Sequence conflict5591R → D in CAA13068. Ref.1
Sequence conflict5591R → D in CAA13069. Ref.1
Sequence conflict713 – 7197SQHRLEL → AASAEA in CAA13068. Ref.1
Sequence conflict7331A → V in CAA13068. Ref.1
Sequence conflict7331A → V in CAA13069. Ref.1
Sequence conflict8561A → V in CAA13068. Ref.1
Sequence conflict8561A → V in CAA13069. Ref.1
Sequence conflict10031N → D in AAH39162. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 27, 2005. Version 2.
Checksum: 8F46948122F58872

FASTA1,047115,910
        10         20         30         40         50         60 
MQKPSGLKPP GRGGKHSSPV GRPSVGSASS SVVASTSGSK EGSPLHKQAS GPSSSGAATT 

        70         80         90        100        110        120 
VSEKPGPKAA EVGDDFLGDF VVGERVWVNG VKPGVVQYLG ETQFAPGQWA GVVLDDPVGK 

       130        140        150        160        170        180 
NDGAVGGVRY FECPALQGIF TRPSKLTRQP TAEGSGSDTH SVESLTAQNL SLHSGTATPP 

       190        200        210        220        230        240 
LTGRVIPLRE SVLNSSVKTG NESGSNLSDS GSVKRGDKDL HLGDRVLVGG TKTGVVRYVG 

       250        260        270        280        290        300 
ETDFAKGEWC GVELDEPLGK NDGAVAGTRY FQCPPKFGLF APIHKVIRIG FPSTSPAKAK 

       310        320        330        340        350        360 
KTKRMAMGVS ALTHSPSSSS ISSVSSVASS VGGRPSRSGL LTETSSRYAR KISGTTALQE 

       370        380        390        400        410        420 
ALKEKQQHIE QLLAERDLER AEVAKATSHI CEVEKEIALL KAQHEQYVAE AEEKLQRARL 

       430        440        450        460        470        480 
LVENVRKEKV DLSNQLEEER RKVEDLQFRV EEESITKGDL ETQTQLEHAR IGELEQSLLL 

       490        500        510        520        530        540 
EKAQAERLLR ELADNRLTTV AEKSRVLQLE EELSLRRGEI EELQHCLLQS GPPPADHPEA 

       550        560        570        580        590        600 
AETLRLRERL LSASKEHQRD STLLQDKYEH MLKTYQTEVD KLRAANEKYA QEVADLKAKV 

       610        620        630        640        650        660 
QQATTENMGL MDNWKSKLDS LASDHQKSLE DLKATLNSGP GAQQKEIGEL KALVEGIKME 

       670        680        690        700        710        720 
HQLELGNLQA KHDLETAMHG KEKEGLRQKL QEVQEELAGL QQHWREQLEE QASQHRLELQ 

       730        740        750        760        770        780 
EAQDQCRDAQ LRAQELEGLD VEYRGQAQAI EFLKEQISLA EKKMLDYEML QRAEAQSRQE 

       790        800        810        820        830        840 
AERLREKLLV AENRLQAAES LCSAQHSHVI ESSDLSEETI RMKETVEGLQ DKLNKRDKEV 

       850        860        870        880        890        900 
TALTSQMDML RAQVSALENK CKSGEKKIDS LLKEKRRLEA ELEAVSRKTH DASGQLVHIS 

       910        920        930        940        950        960 
QELLRKERSL NELRVLLLEA NRHSPGPERD LSREVHKAEW RIKEQKLKDD IRGLREKLTG 

       970        980        990       1000       1010       1020 
LDKEKSLSEQ RRYSLIDPAS PPELLKLQHQ LVSTEDALRD ALNQAQQVER LVEALRGCSD 

      1030       1040 
RTQTISNSGS ANGIHQPDKA HKQEDKH

« Hide

Isoform 2 [UniParc].

Checksum: 5CD0F481CFE7CFB0
Show »

FASTA1,012111,855

References

« Hide 'large scale' references
[1]"The murine CYLN2 gene: genomic organization, chromosome localization, and comparison to the human gene that is located within the 7q11.23 Williams syndrome critical region."
Hoogenraad C.C., Eussen B.H.J., Langeveld A., van Haperen R., Winterberg S., Wouters C.H., Grosveld F., de Zeeuw C.I., Galjart N.
Genomics 53:348-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[2]Green E.D.
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary tumor.
[4]"Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts."
Akhmanova A., Hoogenraad C.C., Drabek K., Stepanova T., Dortland B., Verkerk T., Vermeulen W., Burgering B.M., de Zeeuw C.I., Grosveld F., Galjart N.
Cell 104:923-935(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CLASP1 AND CLASP2.
[5]"CLIP-115, a novel brain specific cytoplasmic linker protein, mediates the localisation of dendritic lamellar bodies."
de Zeeuw C.I., Hoogenraad C.C., Goedknegt E., Hertzberg E., Neubauer A., Grosveld F.G., Galjart N.J.
Neuron 19:1187-1199(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, MASS SPECTROMETRY.
Tissue: Brain cortex.
[7]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, MASS SPECTROMETRY.
Tissue: Melanoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ228863 Genomic DNA. Translation: CAA13068.1.
AJ228865 expand/collapse EMBL AC list , AJ228868, AJ228869, AJ228870, AJ228872, AJ228876, AJ228880, AJ228867, AJ228875, AJ228871, AJ228873, AJ228877, AJ228866, AJ228879, AJ228878, AJ228874 Genomic DNA. Translation: CAA13069.1.
AF289667 Genomic DNA. Translation: AAF99340.1.
AF289664 Genomic DNA. Translation: AAF99333.1.
BC039162 mRNA. Translation: AAH39162.1.
BC053048 mRNA. Translation: AAH53048.1.
IPIIPI00408543.
IPI00467854.
PIRT42720.
RefSeqNP_001034251.1. NM_001039162.1.
NP_034120.2. NM_009990.2.
UniGeneMm.255138.

3D structure databases

ProteinModelPortalQ9Z0H8.
SMRQ9Z0H8. Positions 30-156, 174-345.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9Z0H8. 4 interactions.

PTM databases

PhosphoSiteQ9Z0H8.

Proteomic databases

PaxDbQ9Z0H8.
PRIDEQ9Z0H8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000036999; ENSMUSP00000037431; ENSMUSG00000063146.
ENSMUST00000100647; ENSMUSP00000098212; ENSMUSG00000063146.
GeneID269713.
KEGGmmu:269713.
UCSCuc008zwi.1. mouse.
uc008zwj.1. mouse.

Organism-specific databases

CTD7461.
MGIMGI:1313136. Clip2.
RougeSearch...

Phylogenomic databases

eggNOGCOG5244.
GeneTreeENSGT00700000104055.
HOGENOMHOG000092755.
HOVERGENHBG007123.
InParanoidQ9Z0H8.
KOK10422.
OMAHWRAQLE.
OrthoDBEOG4X3H0M.

Gene expression databases

BgeeQ9Z0H8.
CleanExMM_CLIP2.
GenevestigatorQ9Z0H8.
GermOnlineENSMUSG00000063146. Mus musculus.

Family and domain databases

Gene3D2.30.30.190. 2 hits.
InterProIPR000938. CAP-Gly_domain.
[Graphical view]
PfamPF01302. CAP_GLY. 2 hits.
[Graphical view]
SMARTSM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMSSF74924. CAP-Gly_domain. 2 hits.
PROSITEPS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio393005.
SOURCESearch...

Entry information

Entry nameCLIP2_MOUSE
AccessionPrimary (citable) accession number: Q9Z0H8
Secondary accession number(s): Q7TSI9, Q8CHU1, Q9EP81
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents