Q9Z0G2 (SRPK3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SRSF protein kinase 3
EC=2.7.11.1

Alternative name(s):
Muscle-specific serine kinase 1
Short name=MSSK-1
Serine/arginine-rich protein-specific kinase 3
Short name=SR-protein-specific kinase 3
Serine/threonine-protein kinase 23

Gene names

Name:	Srpk3
Synonyms:	Mssk1, Stk23

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains. Phosphorylates the SR splicing factor SRSF1 and the lamin-B receptor (LBR) in vitro. Required for normal muscle development. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Exclusively expressed in skeletal and heart muscle. Ref.3
Developmental stage	Expressed from embryogenesis to adulthood. Ref.3
Disruption phenotype	Mice are viable to adulthood but display defects in skeletal muscle growth including reduced muscle mass, marked increase in centrally placed nuclei and disorganized intermyofibrillar network. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Differentiation Myogenesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW skeletal muscle tissue development Inferred from mutant phenotype Ref.3. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay Ref.3. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	SRSF protein kinase 3		PRO_0000086710
Regions
	Domain	78 – 563	486	Protein kinase
	Nucleotide binding	84 – 92	9	ATP By similarity
	Compositional bias	16 – 20	5	Poly-Ser
Sites
	Active site	211	1	Proton acceptor By similarity
	Binding site	107	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z0G2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 34976D08141C5DC8	FASTA	565	62,365
	10 20 30 40 50 60 MSANAGGSGS VDCGGSSSSS QTSCGPESSG SELTPATPAP RLLQGLLGSD DEEQEDPKDY 70 80 90 100 110 120 CKGGYYPVKI GDLFNGRYHV VRKLGWGHFS TVWLCWDIQR KRFVALKVVK SAGHYTETAV 130 140 150 160 170 180 DEIKLLKCVR DSDPSDPKRE TIVQLIDDFR ISGVNGVHVC MVLEVLGHQL LKWIIKSNYQ 190 200 210 220 230 240 GLPVPCVKSI VRQVLHGLDY LHTKCKIIHT DIKPENILLC VGDAYIRRLA AEATEWQQSG 250 260 270 280 290 300 AQPPSRSTVS TAPQEVLIGK LSKNKRKKMR RKRKQQKRLL EERLRDLQRL EAMEAAVQAE 310 320 330 340 350 360 DSSSRLERGS GSTSSSGCHP EGTRAGPSPA SSSPVPGGER SLSPSSQTSG FSGSLFSTAS 370 380 390 400 410 420 CSILSGSSNQ RETGGLLSPS TPFGASNLLV NPLEPQNADK IKIKIADLGN ACWVHKHFTE 430 440 450 460 470 480 DIQTRQYRAV EVLIGAEYGP PADIWSTACM AFELATGDYL FEPHSGEDYS RDEDHIAHIV 490 500 510 520 530 540 ELLGDIPPAF ALSGRYSREF FNRRGELRHI PNLKHWGLYE VLMEKYEWPL EQATQFSAFL 550 560 LPMMEYIPEK RASAADCLQH PWLNP

References

[1]	"Cloning and sequencing of a new muscle-specific serine kinase gene in human and mouse." Brenner V., Rosenthal A. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Muscle.
[2]	"Comparative sequence analysis of the mouse L1cam locus and the corresponding region of human Xq28." Platzer M., Brenner V., Reichwald K., Wiehe T., Oksche A., Rosenthal A. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Centronuclear myopathy in mice lacking a novel muscle-specific protein kinase transcriptionally regulated by MEF2." Nakagawa O., Arnold M., Nakagawa M., Hamada H., Shelton J.M., Kusano H., Harris T.M., Childs G., Campbell K.P., Richardson J.A., Nishino I., Olson E.N. Genes Dev. 19:2066-2077(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043289 Genomic DNA. Translation: AAD02248.1. AF043288 mRNA. Translation: AAD02247.1. AF133093 Genomic DNA. No translation available.
IPI	IPI00129112.
RefSeq	NP_062658.1. NM_019684.1.
UniGene	Mm.111904.
3D structure databases
ProteinModelPortal	Q9Z0G2.
SMR	Q9Z0G2. Positions 67-232, 348-564.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z0G2.
Proteomic databases
PaxDb	Q9Z0G2.
PRIDE	Q9Z0G2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000002081; ENSMUSP00000002081; ENSMUSG00000002007.
GeneID	56504.
KEGG	mmu:56504.
Organism-specific databases
CTD	26576.
MGI	MGI:1891338. Srpk3.
Phylogenomic databases
eggNOG	NOG266081.
GeneTree	ENSGT00530000063566.
HOGENOM	HOG000171558.
HOVERGEN	HBG108512.
InParanoid	Q9Z0G2.
KO	K08832.
OrthoDB	EOG4SXNCR.
Gene expression databases
ArrayExpress	Q9Z0G2.
Bgee	Q9Z0G2.
CleanEx	MM_SRPK3.
Genevestigator	Q9Z0G2.
GermOnline	ENSMUSG00000002007. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 2 hits. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	312802.
SOURCE	Search...

Entry information

Entry name

SRPK3_MOUSE

Accession

Primary (citable) accession number: Q9Z0G2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program