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Protein

Cholesterol 25-hydroxylase

Gene

Ch25h

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed:9852097). In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation (PubMed:9852097).2 Publications

Catalytic activityi

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.1 Publication

Cofactori

Fe cation1 Publication

GO - Molecular functioni

  • C-4 methylsterol oxidase activity Source: GO_Central
  • cholesterol 25-hydroxylase activity Source: UniProtKB
  • iron ion binding Source: InterPro
  • steroid hydroxylase activity Source: MGI

GO - Biological processi

  • B cell chemotaxis Source: UniProtKB
  • cholesterol metabolic process Source: MGI
  • fatty acid biosynthetic process Source: InterPro
  • sterol biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.99.38. 3474.
ReactomeiR-MMU-192105. Synthesis of bile acids and bile salts.

Chemistry databases

SwissLipidsiSLP:000001484.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 25-hydroxylase (EC:1.14.99.38)
Alternative name(s):
Cholesterol 25-monooxygenase
Short name:
m25OH
Gene namesi
Name:Ch25h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1333869. Ch25h.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice do not display any apparent alteration in bile acid synthesis and cholesterol metabolism (PubMed:12543708).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi242 – 243HH → EE: Loss of function. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002268021 – 298Cholesterol 25-hydroxylaseAdd BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi5N-linked (GlcNAc...)Sequence analysis1
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9Z0F5.
PRIDEiQ9Z0F5.

Expressioni

Tissue specificityi

Widely expressed at low level and at higher level in the lung. Weakly expressed in the heart, lung and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000050370.
CleanExiMM_CH25H.
GenevisibleiQ9Z0F5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049683.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0F5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 146Histidine box-15
Motifi157 – 161Histidine box-25
Motifi238 – 244Histidine box-37

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000015767.
HOVERGENiHBG080944.
InParanoidiQ9Z0F5.
KOiK10223.
OMAiYFLWHLL.
OrthoDBiEOG091G0CY4.
PhylomeDBiQ9Z0F5.
TreeFamiTF353265.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z0F5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCYNGSELQ DLGCSSQLLL QPLWDTIRTR EAFTRSPIFP VTFSIITYVG
60 70 80 90 100
FCLPFVVLDV LYPWVPILRR YKIHPDFSPS VKQLLPCLGL TLYQHLVFVF
110 120 130 140 150
PVTLLHWVRS PALLPQEAPE LVQLLSHVLI CLLLFDTEIF AWHLLHHKVP
160 170 180 190 200
WLYRTFHKVH HQNSSSFALA TQYMSFWELL SLTFFDVLNV AVLRCHPLTI
210 220 230 240 250
FTFHVINIWL SVEDHSGYDF PWSTHRLVPF GWYGGVAHHD MHHSQFNCNF
260 270 280 290
APYFTHWDKM LGTLRSAPLP ESLCACGERC VNSRERCAVH LIQKKKQT
Length:298
Mass (Da):34,672
Last modified:May 1, 1999 - v1
Checksum:iBBCE4A97A20284C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122V → A in AAH39919 (PubMed:15489334).Curated1
Sequence conflicti199T → S in BAE35945 (PubMed:16141072).Curated1
Sequence conflicti282N → D in AAH39919 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059211 Genomic DNA. Translation: AAC97480.1.
AF059213 mRNA. Translation: AAC97482.1.
AK140360 mRNA. Translation: BAE24352.1.
AK152770 mRNA. Translation: BAE31482.1.
AK160657 mRNA. Translation: BAE35945.1.
BC039919 mRNA. Translation: AAH39919.1.
CCDSiCCDS29759.1.
RefSeqiNP_034020.1. NM_009890.1.
UniGeneiMm.30824.

Genome annotation databases

EnsembliENSMUST00000050562; ENSMUSP00000049683; ENSMUSG00000050370.
GeneIDi12642.
KEGGimmu:12642.
UCSCiuc008hgj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059211 Genomic DNA. Translation: AAC97480.1.
AF059213 mRNA. Translation: AAC97482.1.
AK140360 mRNA. Translation: BAE24352.1.
AK152770 mRNA. Translation: BAE31482.1.
AK160657 mRNA. Translation: BAE35945.1.
BC039919 mRNA. Translation: AAH39919.1.
CCDSiCCDS29759.1.
RefSeqiNP_034020.1. NM_009890.1.
UniGeneiMm.30824.

3D structure databases

ProteinModelPortaliQ9Z0F5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049683.

Chemistry databases

SwissLipidsiSLP:000001484.

Proteomic databases

PaxDbiQ9Z0F5.
PRIDEiQ9Z0F5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050562; ENSMUSP00000049683; ENSMUSG00000050370.
GeneIDi12642.
KEGGimmu:12642.
UCSCiuc008hgj.1. mouse.

Organism-specific databases

CTDi9023.
MGIiMGI:1333869. Ch25h.

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000015767.
HOVERGENiHBG080944.
InParanoidiQ9Z0F5.
KOiK10223.
OMAiYFLWHLL.
OrthoDBiEOG091G0CY4.
PhylomeDBiQ9Z0F5.
TreeFamiTF353265.

Enzyme and pathway databases

BRENDAi1.14.99.38. 3474.
ReactomeiR-MMU-192105. Synthesis of bile acids and bile salts.

Miscellaneous databases

PROiQ9Z0F5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050370.
CleanExiMM_CH25H.
GenevisibleiQ9Z0F5. MM.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCH25H_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0F5
Secondary accession number(s): Q3TUM6, Q8CHQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.