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Q9Z0E2 (CHRD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chordin
Gene names
Name:Chrd
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length948 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.

Subunit structure

Interacts with TWSG1 and/or BMP4. Ref.3

Subcellular location

Secreted By similarity.

Developmental stage

Detected at high levels of a in 7 dpc mouse embryos; its level decrease at later developmental stages and in adult tissues.

Post-translational modification

Cleaved by tolloid proteases; cleavage participates in dorsoventral patterning during early development By similarity.

Sequence similarities

Belongs to the chordin family.

Contains 4 CHRD domains.

Contains 4 VWFC domains.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
Signal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway involved in spinal cord dorsal/ventral patterning

Inferred from electronic annotation. Source: Ensembl

central nervous system development

Inferred from mutant phenotype PubMed 10688202. Source: MGI

dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 12810603. Source: MGI

forebrain development

Inferred from mutant phenotype PubMed 12397106. Source: MGI

gastrulation with mouth forming second

Inferred from mutant phenotype PubMed 12810603. Source: MGI

mesoderm formation

Inferred from mutant phenotype PubMed 12810603. Source: MGI

negative regulation of BMP signaling pathway

Inferred from direct assay PubMed 15780974PubMed 19850029. Source: MGI

negative regulation of cell migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of osteoblast differentiation

Inferred from direct assay PubMed 15780974. Source: MGI

osteoblast differentiation

Inferred from direct assay PubMed 15381701. Source: MGI

pattern specification process

Inferred from mutant phenotype PubMed 12397106. Source: MGI

positive regulation of cell adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of mesenchymal cell proliferation

Inferred from electronic annotation. Source: Ensembl

skeletal system development

Inferred from mutant phenotype PubMed 11784076. Source: MGI

   Cellular_componentextracellular space

Inferred from direct assay PubMed 15780974. Source: MGI

   Molecular_functionheparin binding

Inferred from direct assay PubMed 15381701. Source: MGI

syndecan binding

Inferred from direct assay PubMed 15381701. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 948922Chordin
PRO_0000005365

Regions

Domain49 – 12678VWFC 1
Domain168 – 277110CHRD 1
Domain279 – 398120CHRD 2
Domain399 – 520122CHRD 3
Domain526 – 646121CHRD 4
Domain699 – 75961VWFC 2
Domain779 – 84567VWFC 3
Domain867 – 92761VWFC 4

Amino acid modifications

Glycosylation3471N-linked (GlcNAc...) Potential
Glycosylation4301N-linked (GlcNAc...) Potential
Glycosylation8771N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9Z0E2 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 4DC2DA01D9BD2147

FASTA948101,513
        10         20         30         40         50         60 
MPSLPAPPAP RLLLGLLLLG SRPASGTGPE PPALPIRSEK EPLPVRGAAG CSFGGKVYAL 

        70         80         90        100        110        120 
DETWHPDLGE PFGVMRCVLC ACEAPQWARR GRGPGRVSCK NIKPQCPTLA CRQPRQLPGH 

       130        140        150        160        170        180 
CCQTCPQERS NLDPQPAGLV FEYPRDPEHR SYSDRGEPGV GERTRADGHT DFVALLTGPR 

       190        200        210        220        230        240 
SQAVARARVS LLRSSLRFSV SYQRLDRPSR VRFTDPTGNI LFEHPATPTQ DGLVCGVWRA 

       250        260        270        280        290        300 
VPRLSVRLLR AEQLRVALVT STHPSGEVWG PLIWQGALAA ETFSAILTLE DPLQRGVGGI 

       310        320        330        340        350        360 
ALLTLSDTED SLHFLLLFRG LLGGLAQAPL KLQILHQGQL LRELQANTSA QEPGFAEVLP 

       370        380        390        400        410        420 
SLTDQEMDWL ELGELQMVLE KAGGPELRIS GYITTRQSCD VLQSVLCGAD ALIPVQTGAA 

       430        440        450        460        470        480 
GSASFILLGN GSLIYQVQVV GTGSEVVAMT LETKPQRKNQ RTVLCHMAGL QPGGHMAVGM 

       490        500        510        520        530        540 
CSGLGARGAH MLLQNELFLN VGTKDFPDGE LRGHVTALCY SGHSARYDRL PVPLAGALVL 

       550        560        570        580        590        600 
PPVRSQAAGH AWLSLDTHCH LHYEVLLAGL GGSEQGTVTA HLLGPPGMPG PQRLLKGFYG 

       610        620        630        640        650        660 
SEAQGVVKDL EPVLLRHLAQ GTASLLITTK SSPRGELRGQ VHIASQCEAG GLRLASEGVQ 

       670        680        690        700        710        720 
MPLAPNGEAA TSPMLPAGPG PEAPVPAKHG SPGRPRDPNT CFFEGQQRPH GARWAPNYDP 

       730        740        750        760        770        780 
LCSLCICQRR TVICDPVVCP PPSCPHPVQA LDQCCPVCPE KQRSRDLPSL PNLEPGEGCY 

       790        800        810        820        830        840 
FDGDRSWRAA GTRWHPVVPP FGLIKCAVCT CKGATGEVHC EKVQCPRLAC AQPVRANPTD 

       850        860        870        880        890        900 
CCKQCPVGSG TNAKLGDPMQ ADGPRGCRFA GQWFPENQSW HPSVPPFGEM SCITCRCGAG 

       910        920        930        940 
VPHCERDDCS PPLSCGSGKE SRCCSHCTAQ RSSETRTLPE LEKEAEHS 

« Hide

References

[1]"BMP-binding domains in the chordin secreted protein."
Lu B., Bachiller D., Agius E., Piccolo S., De Robertis E.M.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: B6SJL/F1.
[2]"Coding sequence and expression patterns of mouse chordin and mapping of the cognate mouse chrd and human CHRD genes."
Pappano W.N., Scott I.C., Clark T.G., Eddy R.L., Shows T.B., Greenspan D.S.
Genomics 52:236-239(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Homologues of Twisted gastrulation are extracellular cofactors in antagonism of BMP signalling."
Scott I.C., Blitz I.L., Pappano W.N., Maas S.A., Cho K.W.Y., Greenspan D.S.
Nature 410:475-478(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TWSG1 AND BMP4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF096276 mRNA. Translation: AAD19895.1.
AF069501 mRNA. Translation: AAC68867.1.
RefSeqNP_001264970.1. NM_001278041.1.
NP_034023.1. NM_009893.2.
UniGeneMm.20457.

3D structure databases

ProteinModelPortalQ9Z0E2.
SMRQ9Z0E2. Positions 46-131, 696-763, 774-851, 862-933.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000007171.

PTM databases

PhosphoSiteQ9Z0E2.

Proteomic databases

PRIDEQ9Z0E2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000007171; ENSMUSP00000007171; ENSMUSG00000006958.
GeneID12667.
KEGGmmu:12667.
UCSCuc007yre.2. mouse.

Organism-specific databases

CTD8646.
MGIMGI:1313268. Chrd.

Phylogenomic databases

eggNOGNOG85639.
GeneTreeENSGT00730000110792.
HOVERGENHBG005330.
InParanoidQ9Z0E2.
KOK04657.
OMARDPGEGC.
OrthoDBEOG7QNVK7.
TreeFamTF106451.

Gene expression databases

BgeeQ9Z0E2.
CleanExMM_CHRD.
GenevestigatorQ9Z0E2.

Family and domain databases

InterProIPR016353. Chordin.
IPR010895. CHRD.
IPR001007. VWF_C.
[Graphical view]
PfamPF07452. CHRD. 4 hits.
PF00093. VWC. 3 hits.
[Graphical view]
PIRSFPIRSF002496. Chordin. 1 hit.
SMARTSM00754. CHRD. 4 hits.
SM00214. VWC. 4 hits.
[Graphical view]
PROSITEPS50933. CHRD. 4 hits.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio281902.
PMAP-CutDBQ9Z0E2.
PROQ9Z0E2.
SOURCESearch...

Entry information

Entry nameCHRD_MOUSE
AccessionPrimary (citable) accession number: Q9Z0E2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot