Q9Z0E2 (CHRD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chordin

Gene names

Name:

Chrd

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	948 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.
Subunit structure	Interacts with TWSG1 and/or BMP4. Ref.3
Subcellular location	Secreted By similarity.
Developmental stage	Detected at high levels of a in 7 dpc mouse embryos; its level decrease at later developmental stages and in adult tissues.
Post-translational modification	Cleaved by tolloid proteases; cleavage participates in dorsoventral patterning during early development By similarity.
Sequence similarities	Belongs to the chordin family. Contains 4 CHRD domains. Contains 4 VWFC domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway involved in spinal cord dorsal/ventral patterning Inferred from electronic annotation. Source: Compara dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 12810603. Source: MGI forebrain development Inferred from mutant phenotype PubMed 12397106. Source: MGI gastrulation with mouth forming second Inferred from mutant phenotype PubMed 12810603. Source: MGI mesoderm formation Inferred from mutant phenotype PubMed 12810603. Source: MGI negative regulation of BMP signaling pathway Inferred from direct assay PubMed 15780974PubMed 19850029. Source: MGI negative regulation of cell migration Inferred from electronic annotation. Source: Compara negative regulation of osteoblast differentiation Inferred from direct assay PubMed 15780974. Source: MGI osteoblast differentiation Inferred from direct assay PubMed 15381701. Source: MGI positive regulation of cell adhesion Inferred from electronic annotation. Source: Compara positive regulation of mesenchymal cell proliferation Inferred from electronic annotation. Source: Compara skeletal system development Inferred from mutant phenotype PubMed 11784076. Source: MGI
Cellular_component	extracellular space Inferred from direct assay PubMed 15780974. Source: MGI
Molecular_function	heparin binding Inferred from direct assay PubMed 15381701. Source: MGI syndecan binding Inferred from direct assay PubMed 15381701. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 948	922	Chordin		PRO_0000005365
Regions
	Domain	49 – 126	78	VWFC 1
	Domain	168 – 277	110	CHRD 1
	Domain	279 – 398	120	CHRD 2
	Domain	399 – 520	122	CHRD 3
	Domain	526 – 646	121	CHRD 4
	Domain	699 – 759	61	VWFC 2
	Domain	779 – 845	67	VWFC 3
	Domain	867 – 927	61	VWFC 4
Amino acid modifications
	Glycosylation	347	1	N-linked (GlcNAc...) Potential
	Glycosylation	430	1	N-linked (GlcNAc...) Potential
	Glycosylation	877	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Z0E2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 4DC2DA01D9BD2147	FASTA	948	101,513
	10 20 30 40 50 60 MPSLPAPPAP RLLLGLLLLG SRPASGTGPE PPALPIRSEK EPLPVRGAAG CSFGGKVYAL 70 80 90 100 110 120 DETWHPDLGE PFGVMRCVLC ACEAPQWARR GRGPGRVSCK NIKPQCPTLA CRQPRQLPGH 130 140 150 160 170 180 CCQTCPQERS NLDPQPAGLV FEYPRDPEHR SYSDRGEPGV GERTRADGHT DFVALLTGPR 190 200 210 220 230 240 SQAVARARVS LLRSSLRFSV SYQRLDRPSR VRFTDPTGNI LFEHPATPTQ DGLVCGVWRA 250 260 270 280 290 300 VPRLSVRLLR AEQLRVALVT STHPSGEVWG PLIWQGALAA ETFSAILTLE DPLQRGVGGI 310 320 330 340 350 360 ALLTLSDTED SLHFLLLFRG LLGGLAQAPL KLQILHQGQL LRELQANTSA QEPGFAEVLP 370 380 390 400 410 420 SLTDQEMDWL ELGELQMVLE KAGGPELRIS GYITTRQSCD VLQSVLCGAD ALIPVQTGAA 430 440 450 460 470 480 GSASFILLGN GSLIYQVQVV GTGSEVVAMT LETKPQRKNQ RTVLCHMAGL QPGGHMAVGM 490 500 510 520 530 540 CSGLGARGAH MLLQNELFLN VGTKDFPDGE LRGHVTALCY SGHSARYDRL PVPLAGALVL 550 560 570 580 590 600 PPVRSQAAGH AWLSLDTHCH LHYEVLLAGL GGSEQGTVTA HLLGPPGMPG PQRLLKGFYG 610 620 630 640 650 660 SEAQGVVKDL EPVLLRHLAQ GTASLLITTK SSPRGELRGQ VHIASQCEAG GLRLASEGVQ 670 680 690 700 710 720 MPLAPNGEAA TSPMLPAGPG PEAPVPAKHG SPGRPRDPNT CFFEGQQRPH GARWAPNYDP 730 740 750 760 770 780 LCSLCICQRR TVICDPVVCP PPSCPHPVQA LDQCCPVCPE KQRSRDLPSL PNLEPGEGCY 790 800 810 820 830 840 FDGDRSWRAA GTRWHPVVPP FGLIKCAVCT CKGATGEVHC EKVQCPRLAC AQPVRANPTD 850 860 870 880 890 900 CCKQCPVGSG TNAKLGDPMQ ADGPRGCRFA GQWFPENQSW HPSVPPFGEM SCITCRCGAG 910 920 930 940 VPHCERDDCS PPLSCGSGKE SRCCSHCTAQ RSSETRTLPE LEKEAEHS

References

[1]	"BMP-binding domains in the chordin secreted protein." Lu B., Bachiller D., Agius E., Piccolo S., De Robertis E.M. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: B6SJL/F1.
[2]	"Coding sequence and expression patterns of mouse chordin and mapping of the cognate mouse chrd and human CHRD genes." Pappano W.N., Scott I.C., Clark T.G., Eddy R.L., Shows T.B., Greenspan D.S. Genomics 52:236-239(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Homologues of Twisted gastrulation are extracellular cofactors in antagonism of BMP signalling." Scott I.C., Blitz I.L., Pappano W.N., Maas S.A., Cho K.W.Y., Greenspan D.S. Nature 410:475-478(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TWSG1 AND BMP4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF096276 mRNA. Translation: AAD19895.1. AF069501 mRNA. Translation: AAC68867.1.
IPI	IPI00129023.
RefSeq	NP_034023.1. NM_009893.2.
UniGene	Mm.20457.
3D structure databases
ProteinModelPortal	Q9Z0E2.
SMR	Q9Z0E2. Positions 47-131, 696-762, 777-848, 863-934.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000007171.
PTM databases
PhosphoSite	Q9Z0E2.
Proteomic databases
PRIDE	Q9Z0E2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000007171; ENSMUSP00000007171; ENSMUSG00000006958.
GeneID	12667.
KEGG	mmu:12667.
Organism-specific databases
CTD	8646.
MGI	MGI:1313268. Chrd.
Phylogenomic databases
eggNOG	NOG85639.
GeneTree	ENSGT00700000104447.
HOVERGEN	HBG005330.
InParanoid	Q9Z0E2.
KO	K04657.
OMA	GARWAPN.
OrthoDB	EOG4T1HKS.
Gene expression databases
ArrayExpress	Q9Z0E2.
Bgee	Q9Z0E2.
CleanEx	MM_CHRD.
Genevestigator	Q9Z0E2.
GermOnline	ENSMUSG00000006958. Mus musculus.
Family and domain databases
InterPro	IPR016353. Chordin. IPR010895. CHRD. IPR001007. VWF_C. [Graphical view]
Pfam	PF07452. CHRD. 4 hits. PF00093. VWC. 3 hits. [Graphical view]
PIRSF	PIRSF002496. Chordin. 1 hit.
SMART	SM00754. CHRD. 4 hits. SM00214. VWC. 4 hits. [Graphical view]
PROSITE	PS50933. CHRD. 4 hits. PS01208. VWFC_1. 2 hits. PS50184. VWFC_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	281902.
PMAP-CutDB	Q9Z0E2.
SOURCE	Search...

Entry information

Entry name

CHRD_MOUSE

Accession

Primary (citable) accession number: Q9Z0E2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program