Q9Z0D9 (CX3C1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CX3C chemokine receptor 1
Short name=C-X3-C CKR-1
Short name=CX3CR1

Alternative name(s):
Fractalkine receptor

Gene names

Name:

Cx3cr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	This protein is not N-glycosylated which is unusual for G-protein-coupled receptors.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara cellular response to transforming growth factor beta stimulus Inferred from electronic annotation. Source: Compara cerebral cortex cell migration Inferred from mutant phenotype PubMed 16732273. Source: MGI chemotaxis Inferred from electronic annotation. Source: InterPro cytokine-mediated signaling pathway Inferred from mutant phenotype PubMed 18971423. Source: MGI elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: InterPro macrophage chemotaxis Inferred from mutant phenotype PubMed 18322241. Source: MGI microglial cell activation involved in immune response Inferred from mutant phenotype PubMed 16732273. Source: MGI negative regulation of angiogenesis Inferred from mutant phenotype PubMed 18322241. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype PubMed 18971423. Source: MGI negative regulation of cell migration Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from electronic annotation. Source: Compara neuronal cell body membrane Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	C-X3-C chemokine receptor activity Inferred from electronic annotation. Source: Compara cytokine receptor activity Inferred from mutant phenotype PubMed 18971423. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	CX3C chemokine receptor 1		PRO_0000069327
Regions
	Topological domain	1 – 32	32	Extracellular Potential
	Transmembrane	33 – 60	28	Helical; Name=1; Potential
	Topological domain	61 – 70	10	Cytoplasmic Potential
	Transmembrane	71 – 91	21	Helical; Name=2; Potential
	Topological domain	92 – 104	13	Extracellular Potential
	Transmembrane	105 – 126	22	Helical; Name=3; Potential
	Topological domain	127 – 143	17	Cytoplasmic Potential
	Transmembrane	144 – 168	25	Helical; Name=4; Potential
	Topological domain	169 – 196	28	Extracellular Potential
	Transmembrane	197 – 216	20	Helical; Name=5; Potential
	Topological domain	217 – 232	16	Cytoplasmic Potential
	Transmembrane	233 – 257	25	Helical; Name=6; Potential
	Topological domain	258 – 274	17	Extracellular Potential
	Transmembrane	275 – 298	24	Helical; Name=7; Potential
	Topological domain	299 – 354	56	Cytoplasmic Potential
Amino acid modifications
	Disulfide bond	103 ↔ 176		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z0D9 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 12C745E8E3755CA9	FASTA	354	40,266
	10 20 30 40 50 60 MSTSFPELDL ENFEYDDSAE ACYLGDIVAF GTIFLSVFYA LVFTFGLVGN LLVVLALTNS 70 80 90 100 110 120 RKPKSITDIY LLNLALSDLL FVATLPFWTH YLISHEGLHN AMCKLTTAFF FIGFFGGIFF 130 140 150 160 170 180 ITVISIDRYL AIVLAANSMN NRTVQHGVTI SLGVWAAAIL VASPQFMFTK RKDNECLGDY 190 200 210 220 230 240 PEVLQEMWPV LRNSEVNILG FALPLLIMSF CYFRIIQTLF SCKNRKKARA VRLILLVVFA 250 260 270 280 290 300 FFLFWTPYNI MIFLETLKFY NFFPSCDMKR DLRLALSVTE TVAFSHCCLN PFIYAFAGEK 310 320 330 340 350 FRRYLGHLYR KCLAVLCGHP VHTGFSPESQ RSRQDSILSS FTHYTSEGDG SLLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine." Combadiere C., Gao J.-L., Tiffany H.L., Murphy P.M. Biochem. Biophys. Res. Commun. 253:728-732(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[2]	"Cloning and characterization of murine CX3CR1, a receptor for murine fractalkine/neurotactin." Coultas L., McColl S.R. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/SvJ.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF074912 Genomic DNA. Translation: AAD08665.1. AF102269 mRNA. Translation: AAC72408.1. BC012653 mRNA. Translation: AAH12653.1.
IPI	IPI00128996.
RefSeq	NP_034117.3. NM_009987.4.
UniGene	Mm.44065.
3D structure databases
ProteinModelPortal	Q9Z0D9.
SMR	Q9Z0D9. Positions 37-334.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9Z0D9.
Proteomic databases
PaxDb	Q9Z0D9.
PRIDE	Q9Z0D9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000064165; ENSMUSP00000063986; ENSMUSG00000052336. ENSMUST00000177637; ENSMUSP00000136413; ENSMUSG00000052336.
GeneID	13051.
KEGG	mmu:13051.
Organism-specific databases
CTD	1524.
MGI	MGI:1333815. Cx3cr1.
Phylogenomic databases
eggNOG	NOG151350.
GeneTree	ENSGT00700000104251.
HOGENOM	HOG000234122.
HOVERGEN	HBG106917.
InParanoid	Q9Z0D9.
KO	K04192.
OMA	HRRESTF.
OrthoDB	EOG4WM4VB.
Gene expression databases
ArrayExpress	Q9Z0D9.
Bgee	Q9Z0D9.
CleanEx	MM_CX3CR1.
Genevestigator	Q9Z0D9.
Family and domain databases
InterPro	IPR005387. Chemokine_CX3CR1. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01562. FRACTALKINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	282966.
SOURCE	Search...

Entry information

Entry name

CX3C1_MOUSE

Accession

Primary (citable) accession number: Q9Z0D9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program