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Q9Z0D5 (REP1_MDV1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Para-Rep C1

Short name=Rep1
EC=2.7.7.-
EC=3.1.21.-
EC=3.6.1.3
Alternative name(s):
Replication-associated protein of non-essential DNA C1
Gene names
Name:C1
OrganismMilk vetch dwarf virus (isolate N) (MDV) [Complete proteome]
Taxonomic identifier291605 [NCBI]
Taxonomic lineageVirusesssDNA virusesNanoviridaeNanovirus
Virus hostAstragalus sinicus (Chinese milk vetch) [TaxID: 47065]
Glycine max (Soybean) (Glycine hispida) [TaxID: 3847]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Pisum sativum (Garden pea) [TaxID: 3888]
Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese By similarity.

Subunit structure

Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Miscellaneous

The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.

Sequence similarities

Belongs to the nanoviridea/circoviridae replication-associated protein family.

Caution

This protein is encoded by a subviral DNA that is not present in all isolates of the virus.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 281281Para-Rep C1
PRO_0000378519

Regions

Nucleotide binding173 – 1819ATP By similarity
Motif11 – 144RCR-1 By similarity
Motif42 – 476RCR-2 By similarity
Motif51 – 7121Nuclear localization signal Potential
Motif80 – 834RCR-3 By similarity
Motif97 – 1037Nuclear localization signal Potential

Sites

Active site801For DNA cleavage activity By similarity
Metal binding361Divalent metal cation Potential
Metal binding421Divalent metal cation Potential
Metal binding851Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Q9Z0D5 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 3D3AAE74F3A38A06

FASTA28132,870
        10         20         30         40         50         60 
MPTLQGTFWC FTLNFSGDAP SLSFNERVQY ACWQHERVSH DHLQGYIQMK KRSTLKMMKE 

        70         80         90        100        110        120 
LLPGAHLEVS KGTPEEASDY AMKEETRVAG PWTYGELLKK GSNKRKLLDR YKENPEDMEL 

       130        140        150        160        170        180 
EDPAKARRCR AKIDKEKFIA EFKVEDDEQE WKKILEKEIE KIASPRSILW VYGPQGGEGK 

       190        200        210        220        230        240 
TSKAKELITR GWFYTRGGKK DDVAYSYVED PTRHVVFDIP RDMQEYCNYS LIEMLKDRII 

       250        260        270        280 
ISNKYEPITN CQVYNIHVIV MANFLPDVTK ISEDRIKIIY C 

« Hide

References

[1]"Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome."
Sano Y., Wada M., Hashimoto Y., Matsumoto T., Kojima M.
J. Gen. Virol. 79:3111-3118(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB000920 Genomic DNA. Translation: BAA33980.1.

3D structure databases

ProteinModelPortalQ9Z0D5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameREP1_MDV1
AccessionPrimary (citable) accession number: Q9Z0D5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families