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Q9Z0D4

- REP2_MDV1

UniProt

Q9Z0D4 - REP2_MDV1

Protein

Para-Rep C2

Gene

C2

Organism
Milk vetch dwarf virus (isolate N) (MDV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Divalent metal cations, possibly magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi38 – 381Divalent metal cationSequence Analysis
    Metal bindingi47 – 471Divalent metal cationSequence Analysis
    Active sitei86 – 861For DNA cleavage activityBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi174 – 1829ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity, uncoupled Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. DNA binding Source: UniProtKB-KW
    4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. metal ion binding Source: UniProtKB-KW
    6. nucleotidyltransferase activity Source: UniProtKB-KW
    7. RNA binding Source: InterPro
    8. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein-DNA covalent cross-linking Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Para-Rep C2 (EC:2.7.7.-, EC:3.1.21.-, EC:3.6.1.3)
    Short name:
    Rep2
    Alternative name(s):
    Replication-associated protein of non-essential DNA C2
    Gene namesi
    Name:C2
    OrganismiMilk vetch dwarf virus (isolate N) (MDV)
    Taxonomic identifieri291605 [NCBI]
    Taxonomic lineageiVirusesssDNA virusesNanoviridaeNanovirus
    Virus hostiAstragalus sinicus (Chinese milk vetch) [TaxID: 47065]
    Glycine max (Soybean) (Glycine hispida) [TaxID: 3847]
    Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
    Pisum sativum (Garden pea) [TaxID: 3888]
    Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]
    ProteomesiUP000008236: Genome

    Subcellular locationi

    Host nucleus Curated

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 282282Para-Rep C2PRO_0000378522Add
    BLAST

    Keywords - PTMi

    Covalent protein-DNA linkage

    Interactioni

    Subunit structurei

    Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity.By similarityCurated

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z0D4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi8 – 114RCR-1By similarity
    Motifi47 – 526RCR-2By similarity
    Motifi56 – 7722Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi86 – 894RCR-3By similarity
    Motifi99 – 1057Nuclear localization signalSequence Analysis

    Domaini

    There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR003365. Viral_rep_N.
    [Graphical view]
    PfamiPF00910. RNA_helicase. 1 hit.
    PF02407. Viral_Rep. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z0D4-1 [UniParc]FASTAAdd to Basket

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    MASKRWCFTL NYKTALERET FISLFSRDEL NYFVCGDEIA PTTGQKHLQG    50
    YVSMKKLIRL GGLKKKFGSI AHWEIAKGDD FQNRDYCTKE TLIAEIGAPV 100
    KKGSNRRKIM EIYEEDPEEM KLRDPDTALR CKAKKLREEY CSEVSVFSLR 150
    PWQIELHRAL MEVPDDRTII WAYGPDGGEG KSTFAKELIK YGWFYTAGGK 200
    TQDILYMYAQ DPERNIAFDV PRCSSEMMNY QAMEMMKNRV FASTKYRPVD 250
    LCIRKKVHLI VFANVAPDPT KLSEDRIVII NC 282
    Length:282
    Mass (Da):32,682
    Last modified:May 1, 1999 - v1
    Checksum:i6E8CE00C0BB7462E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB000921 Genomic DNA. Translation: BAA33981.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB000921 Genomic DNA. Translation: BAA33981.1 .

    3D structure databases

    ProteinModelPortali Q9Z0D4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR003365. Viral_rep_N.
    [Graphical view ]
    Pfami PF00910. RNA_helicase. 1 hit.
    PF02407. Viral_Rep. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome."
      Sano Y., Wada M., Hashimoto Y., Matsumoto T., Kojima M.
      J. Gen. Virol. 79:3111-3118(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiREP2_MDV1
    AccessioniPrimary (citable) accession number: Q9Z0D4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.

    Caution

    This protein is encoded by a subviral DNA that is not present in all isolates of the virus.Curated

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3