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Q9YXL6

- Q9YXL6_9INFA

UniProt

Q9YXL6 - Q9YXL6_9INFA

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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus H3N2
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.SAAS annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotationSAAS annotation

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymeraseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virusSAAS annotation, Viral RNA replicationSAAS annotation, Viral transcriptionSAAS annotation

Keywords - Ligandi

Nucleotide-bindingSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunitUniRule annotationSAAS annotation (EC:2.7.7.48UniRule annotationSAAS annotation)
Gene namesi
Name:PB1Imported
OrganismiInfluenza A virus H3N2Imported
Taxonomic identifieri41857 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A

PTM / Processingi

Keywords - PTMi

PhosphoproteinSAAS annotation

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.SAAS annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F7PX-ray1.90C496-505[»]
4F7TX-ray1.70C/F498-505[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains RdRp catalytic domain.SAAS annotation

Family and domain databases

InterProiIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamiPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9YXL6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS
60 70 80 90 100
EKGKWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP
110 120 130 140 150
GIFENSCLET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF
160 170 180 190 200
RSNGLTASES GRLIDFLKDV MESMDKEEIE VTTHFQRKRR VRDNMTKKMV
210 220 230 240 250
TQRTIGKKKQ RVNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG
260 270 280 290 300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF
310 320 330 340 350
TITGDNTKWN ENQNPRMFLA MITYITKNQP EWFRNILSIA PIMFSNKMAR
360 370 380 390 400
LGKGYMFESK RMKLRTQIPA EMLASIDLKY FNESTRKKIE KIRPLLIDGT
410 420 430 440 450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKK YTKTTYWWDG LQSSDDFALI
460 470 480 490 500
VNAPNHEGIQ AGVDRFYRTC KLVGINMSKK KSYINKTGTF EFTSFFYRYG
510 520 530 540 550
FVANFSMELP SFGVSGVNES ADMSIGVTVI KNNMINNDLG PATAQMALQL
560 570 580 590 600
FIKDYRYTYR CHRGDTQIQT RRSFELKKLW DQTQSKAGLL VSDGGPNLYN
610 620 630 640 650
IRNLHIPEVC LKWELMDEDY RGRLCNPLNP FVSHKEIESV NNAVVMPAHG
660 670 680 690 700
PAKSMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF
710 720 730 740 750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF SEIMKICSTI

EELRRQK
Length:757
Mass (Da):86,340
Last modified:May 1, 1999 - v1
Checksum:i9AEE58B338AA8BC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037420 Genomic RNA. Translation: AAC63451.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037420 Genomic RNA. Translation: AAC63451.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4F7P X-ray 1.90 C 496-505 [» ]
4F7T X-ray 1.70 C/F 498-505 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view ]
Pfami PF00602. Flu_PB1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Phylogenetic analysis of the entire genome of influenza A (H3N2) viruses from Japan: evidence for genetic reassortment of the six internal genes."
    Lindstrom S.E., Hiromoto Y., Nerome R., Omoe K., Sugita S., Yamazaki Y., Takahashi T., Nerome K.
    J. Virol. 72:8021-8031(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: A/Niigata/137/96Imported.
  2. "Cross-allele cytotoxic T lymphocyte responses against 2009 pandemic H1N1 influenza A virus among HLA-A24 and HLA-A3 supertype-positive individuals."
    Liu J., Zhang S., Tan S., Yi Y., Wu B., Cao B., Zhu F., Wang C., Wang H., Qi J., Gao G.F.
    J. Virol. 86:13281-13294(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 498-505.

Entry informationi

Entry nameiQ9YXL6_9INFA
AccessioniPrimary (citable) accession number: Q9YXL6
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 1999
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3