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Protein

Polymerase acidic protein

Gene

PA

Organism
Influenza A virus H3N2
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi80Manganese 2UniRule annotation1
Metal bindingi108Manganese 1UniRule annotation1
Metal bindingi108Manganese 2UniRule annotation1
Metal bindingi119Manganese 1UniRule annotation1
Metal bindingi120Manganese 1; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonucleaseUniRule annotationSAAS annotation, Hydrolase, Nuclease
Biological processCap snatchingUniRule annotationSAAS annotation, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virusUniRule annotationSAAS annotation
LigandManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase acidic proteinUniRule annotationSAAS annotation (EC:3.1.-.-UniRule annotation)
Alternative name(s):
RNA-directed RNA polymerase subunit P2UniRule annotation
Gene namesi
Name:PAUniRule annotationImported
OrganismiInfluenza A virus H3N2Imported
Taxonomic identifieri41857 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeAlphainfluenzavirus

Subcellular locationi

  • Host nucleus SAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasmUniRule annotationSAAS annotation, Host nucleusUniRule annotationSAAS annotation

PTM / Processingi

Post-translational modificationi

Phosphorylated on serines and threonines by host kinases, including human casein kinase II.UniRule annotation

Keywords - PTMi

PhosphoproteinUniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F7MX-ray2.40C/F649-658[»]
SMRiQ9YXL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili14 – 34Sequence analysisAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi184 – 247Nuclear localization signal 2 (NLS2)UniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the influenza viruses PA family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

Gene3Di3.40.91.901 hit
HAMAPiMF_04063 INFV_PA, 1 hit
InterProiView protein in InterPro
IPR037534 INFV_PA
IPR001009 PA/PA-X
IPR038372 PA/PA-X_sf
PfamiView protein in Pfam
PF00603 Flu_PA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9YXL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD
60 70 80 90 100
FHFINEQGES IVVELDDPNA LLKHRFEIIE GRDRTMAWTV VNSICNTTGA
110 120 130 140 150
EKPKFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS ENTHIHIFSF
160 170 180 190 200
TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMANRGLWDS FRQSERGEET
210 220 230 240 250
IEEKFEISGT MRRLADQSLP PNFSCLENFR AYVDGFEPNG CIEGKLSQMS
260 270 280 290 300
KEVNAKIEPF LKTTPRPIKL PNGPPCYQRS KFLLMDALKL SIEDPSHEGE
310 320 330 340 350
GIPLYDAIKC IRTFFGWKEP YIVKPHEKGI NSNYLLSWKQ VLAELQDIET
360 370 380 390 400
EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDNCRDISDL KQYDSDEPEL
410 420 430 440 450
RSLSSWIQNE FNKACELTDS IWIELDEIGE DIAPIEHIAS MRRNYFTAEV
460 470 480 490 500
SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL
510 520 530 540 550
YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLL
560 570 580 590 600
RSAIGQMSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES
610 620 630 640 650
SVKEKDMTKE FFENKSEAWP IGESPKGVEE GSIGKVCRTL LAKSVFNSLY
660 670 680 690 700
ASPQLEGFSA ESRKLLLVVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV
710
LLNASWFNSF LKHALK
Length:716
Mass (Da):82,765
Last modified:May 1, 1999 - v1
Checksum:i1F2D03926A52ACCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037425 Genomic RNA Translation: AAC63456.1

Keywords - Coding sequence diversityi

Ribosomal frameshiftingSAAS annotation

Similar proteinsi

Entry informationi

Entry nameiQ9YXL3_9INFA
AccessioniPrimary (citable) accession number: Q9YXL3
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 1999
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources