Q9YW39 (NTP1_MSEPV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside triphosphatase I
EC=3.6.1.15

Alternative name(s):
NPH-I
Nucleoside triphosphate phosphohydrolase I
Short name=NPH I

Gene names

Name:	NPH1
Ordered Locus Names:	MSV053

Organism

Melanoplus sanguinipes entomopoxvirus (MsEPV)

Taxonomic identifier

83191 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Entomopoxvirinae › unclassified Entomopoxvirinae

Virus host

Melanoplus sanguinipes (Migratory grasshopper) [TaxID: 65742]

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity.
Subcellular location	Virion By similarity. Note: Virion core enzyme By similarity.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	Nucleoside triphosphatase I		PRO_0000099103
Regions
	Domain	48 – 213	166	Helicase ATP-binding
	Domain	377 – 540	164	Helicase C-terminal
	Nucleotide binding	61 – 68	8	ATP By similarity
	Region	466 – 532	67	Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity
	Motif	150 – 153	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	Q9YW39 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: E78C7F768235D51D	FASTA	647	75,135
	10 20 30 40 50 60 MHSIEHIVAR HFNYALEKTK DLPKSINNEI TNEIIILKDY QYLVSRIFIG LSELNSLLLF 70 80 90 100 110 120 WDTGYGKTLT SVYIMKHLKL VFPQWFFVIF IKKSLYVDPW LNTLSKLGMK GQNIKFVLYD 130 140 150 160 170 180 SSSSLKQFNV LYRTIISSIN TKNRILIIID EVHQVISRTI EKSSSTQRNF LSIFNKIVKL 190 200 210 220 230 240 ANSENNKLLC MSATPITNNV LEFKYLINLL RPKIIEFKED FIVNNTLKNH EQLKNGLISI 250 260 270 280 290 300 TSYQKISEAD SFTNTSYTEG FASKNIFYHN VTMTPEQSNI FNIADKHDKK SALGGLKTMR 310 320 330 340 350 360 RLVSSFAFYD IKIKGSMSQI EYNKMVSEKL NEFKSIIGNF KFSNEFIDIF RNNDSFSNAK 370 380 390 400 410 420 SSEIEIFDKI KQYSCKYIEA CKIILNSNGK VLLYEPLVSF EGISTLKIYF NIFNISYVEY 430 440 450 460 470 480 SSKTESTRDY NIDIFNKYDN LYGNKIKVCI FSAAGSEGIS FSSINDIIIL DLPWKESDIK 490 500 510 520 530 540 QIIGRSIRLN SHEELPIEKR YVNVHFIIAS TIDGKSVDKE IFDLIKSKQD KINVLNSFMK 550 560 570 580 590 600 VISIEQIHSK YKYAEPVENE YIFNNIRHTK IDDVNENNVI TKIIVSPIYY CSEDNLNIIY 610 620 630 640 NGYLDKKTGI IYSNNIPIAK LILDENNIYK FFIKDDKLVY ITKSIYE

References

[1]	"The genome of Melanoplus sanguinipes entomopoxvirus." Afonso C.L., Tulman E.R., Lu Z., Oma E., Kutish G.F., Rock D.L. J. Virol. 73:533-552(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate Tucson.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF063866 Genomic DNA. Translation: AAC97824.1.
PIR	T28214.
RefSeq	NP_048124.1. NC_001993.1.
3D structure databases
ProteinModelPortal	Q9YW39.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1449819.
Phylogenomic databases
ProtClustDB	CLSP2509799.
Family and domain databases
InterPro	IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_MSEPV

Accession

Primary (citable) accession number: Q9YW39

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program