Q9YW06 (NPH2_MSEPV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA helicase NPH-II
EC=3.6.4.13

Alternative name(s):
Nucleoside triphosphatase II
Short name=NTPase II
Nucleoside triphosphate phosphohydrolase II
Short name=NPH II

Gene names

Name:	NPH2
Ordered Locus Names:	MSV086

Organism

Melanoplus sanguinipes entomopoxvirus (MsEPV)

Taxonomic identifier

83191 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Entomopoxvirinae

Virus host

Melanoplus sanguinipes (Migratory grasshopper) [TaxID: 65742]

Protein attributes

Sequence length	717 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NTP-dependent helicase that catalyzes unidirectional unwinding of 3'tailed duplex RNAs and plays an important role during transcription of early mRNAs, presumably by preventing R-loop formation behind the elongating RNA polymerase. Might also play a role in the export of newly synthesized mRNA chains out of the core into the cytoplasm. Required for replication and propagation of viral particles By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Monomer.
Subcellular location	Virion. Note: Localizes to the virion core By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 717	717	RNA helicase NPH-II		PRO_0000055189
Regions
	Domain	193 – 384	192	Helicase ATP-binding
	Domain	406 – 566	161	Helicase C-terminal
	Nucleotide binding	206 – 213	8	ATP By similarity
	Motif	331 – 334	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	Q9YW06 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: E8FB04661F1EA863	FASTA	717	84,726
	10 20 30 40 50 60 MDMQNITDLY KIDKKTTLYP NIINKYNYMA YLLFPNNATI FNSYITKKEV FEYPMQFAIA 70 80 90 100 110 120 LYPVYKLYWH NINICLNNRF IYLSNEFKNN ISINTVYNLL YNNELKFEDD NIIINGKNLK 130 140 150 160 170 180 ISYSAYSYVT IISQITINIS SLNKYQIYGI IESANYLGIL SSYKQNKYFD KNLFSFTKSE 190 200 210 220 230 240 LRSTMIDVQL KIFEIFISKK NCIISGGTGI GKTTVIPKLF WWFNLLFDGY EFWNTSNENK 250 260 270 280 290 300 NINDFIFKPN FEKNKTILSL PRKALIRQMG INYIKSLGFD NISGSPIILK YKDVKKEKEY 310 320 330 340 350 360 YNNNPILYPF VLSVNRITIN NIKHSNSVII DEIHEHDKFG DIAIAIARTK KKKYNIRNIV 370 380 390 400 410 420 LISATIESDI DNIRIYFKNI VEIYIPGVSL FPVKEIECED KDVISILKNY MPSVGKSVII 430 440 450 460 470 480 FYETIKKINE YKEILESILI DKIYKIYTIH SKITNINAII NKLQNDKKHI HIILSTNYLE 490 500 510 520 530 540 SSITITNATL VIDNGKMYQK KFLTGSTMYI TESMYIQRKG RVGRISKGTY IRTYSKDLLQ 550 560 570 580 590 600 TTFKHINYQY LWEYILVFKY NNMDYYNDLF IKPDDPSRIE NTLNYLKNIN IDIDKYISLL 610 620 630 640 650 660 YSKFNKYEIN MVEYLSIYIN NSTSDIILLN EFIDNIRNSD KYIFPYRLTE IFHKLNVRCR 670 680 690 700 710 CINITETEEG NINCSFVILN NYDGDPFFKL SFEKSNLICR YNKIYYIVSM SPLYLID

References

[1]	"The genome of Melanoplus sanguinipes entomopoxvirus." Afonso C.L., Tulman E.R., Lu Z., Oma E., Kutish G.F., Rock D.L. J. Virol. 73:533-552(1999) [PubMed: 9847359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate Tucson.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF063866 Genomic DNA. Translation: AAC97810.1.
PIR	T28247.
RefSeq	NP_048157.1. NC_001993.1.
3D structure databases
ProteinModelPortal	Q9YW06.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1449805.
Phylogenomic databases
ProtClustDB	PHA2653.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. IPR021892. NPH-II. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF12011. DUF3503. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. False negative. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NPH2_MSEPV

Accession

Primary (citable) accession number: Q9YW06

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program