Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9YS17

- NSP4_ROTPC

UniProt

Q9YS17 - NSP4_ROTPC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Non-structural glycoprotein 4

Gene
N/A
Organism
Rotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in virus morphogenesis. Functions as a receptor for the immature double-layered inner capsid particle (ICP) which transiently buds into the lumen of the rough endoplasmic reticulum during viral maturation (By similarity).By similarity
Enterotoxin that causes a phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea. To perform this enterotoxigenic role in vivo, NSP4 is probably released from infected enterocytes in a soluble form capable of diffusing within the intestinal lumen and interacting with the plasma membrane receptors on neighboring epithelial cells (By similarity).By similarity

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Enterotoxin, Toxin

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural glycoprotein 4
Short name:
NSP4
OrganismiRotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Taxonomic identifieri10916 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008175: Genome

Subcellular locationi

Chain Non-structural glycoprotein 4 : Host rough endoplasmic reticulum membrane By similarity; Single-pass type III membrane protein By similarity. Host membranehost caveola; Single-pass type III membrane protein. Secreted By similarity
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles. NSP4 also localizes in vesicular structures, which contain an autophagosomal marker and associate with viroplasms in virus-infected cells (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121LumenalBy similarityAdd
BLAST
Transmembranei22 – 4625HelicalSequence AnalysisAdd
BLAST
Topological domaini47 – 150104CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Non-structural glycoprotein 4PRO_0000369891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili68 – 9023Sequence AnalysisAdd
BLAST

Domaini

The coiled coil region mediates oligomerization.By similarity

Sequence similaritiesi

Belongs to the rotavirus NSP4 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Sequencei

Sequence statusi: Complete.

Q9YS17-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFINQTFFS DYSEGKIDTI PYALGIVLAL TNGSRILKFI NLLISLLRKF
60 70 80 90 100
IITSKTVIGK FKIENNTSHQ NDDIHKEYEE VMKQMREMRV HVTALFDSIH
110 120 130 140 150
KDNMEWRMSE SIRREKKREM KASTAENEVK IHTNDVNICD TSGLETEVCL
Length:150
Mass (Da):17,489
Last modified:May 1, 1999 - v1
Checksum:i5093C34FEFBE8FC2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501F → L in strain: Cowden-virulent.
Natural varianti97 – 971D → N in strain: Cowden-virulent.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093202 mRNA. Translation: AAC83711.1.
AF093203 mRNA. Translation: AAC83712.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093202 mRNA. Translation: AAC83711.1 .
AF093203 mRNA. Translation: AAC83712.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Comparisons of nucleotide and deduced amino acid sequences of NSP4 genes of virulent and attenuated pairs of group A and C rotaviruses."
    Chang K.O., Kim Y.J., Saif L.J.
    Virus Genes 18:229-233(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Cowden-attenuated and Cowden-virulent.

Entry informationi

Entry nameiNSP4_ROTPC
AccessioniPrimary (citable) accession number: Q9YS17
Secondary accession number(s): Q9YS18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3