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Q9YN02

- RPOA_PRRS1

UniProt

Q9YN02 - RPOA_PRRS1

Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Porcine reproductive and respiratory syndrome virus (strain 16244B) (PRRSV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 3 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
    Nsp1 is essential for viral subgenomic mRNA synthesis.By similarity
    Nsp1-alpha inhibits IFN-beta production. Counteracts the action of NF-kappaB by decreasing the phosphorylation of IkappaB-alpha, such that the degradation of IkappaB-alpha is suppressed. This leads to the blockage of NF-kappaB nuclear translocation and thus interference of NF-kappaB activation. Also seems to inhibit IRF3-dependent pathways By similarity.By similarity
    Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity. Deubiquitinates host NFKBIA, thereby interfering with NFKBIA degradation and impairing subsequent NF-kappa-B activation By similarity.By similarity
    The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.By similarity
    The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    ATP + H2O = ADP + phosphate.
    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei76 – 761For Nsp1-alpha papain-like cysteine proteinase activityPROSITE-ProRule annotation
    Active sitei146 – 1461For Nsp1-alpha papain-like cysteine proteinase activityPROSITE-ProRule annotation
    Sitei180 – 1812Cleavage; by autolysisBy similarity
    Active sitei270 – 2701For Nsp1-beta papain-like cysteine proteinase activityPROSITE-ProRule annotation
    Active sitei339 – 3391For Nsp1-beta papain-like cysteine proteinase activityPROSITE-ProRule annotation
    Sitei383 – 3842Cleavage; by autolysisBy similarity
    Active sitei437 – 4371For Nsp2 cysteine proteinase activityPROSITE-ProRule annotation
    Active sitei506 – 5061For Nsp2 cysteine proteinase activityPROSITE-ProRule annotation
    Sitei1579 – 15802Cleavage; by CP2Sequence Analysis
    Sitei1809 – 18102Cleavage; by 3CLSPBy similarity
    Active sitei1848 – 18481Charge relay system; for 3C-like serine proteinase activityPROSITE-ProRule annotation
    Active sitei1873 – 18731Charge relay system; for 3C-like serine proteinase activityPROSITE-ProRule annotation
    Active sitei1927 – 19271Charge relay system; for 3C-like serine proteinase activityPROSITE-ProRule annotation
    Sitei2013 – 20142Cleavage; by 3CLSPBy similarity
    Sitei2183 – 21842Cleavage; by 3CLSPBy similarity
    Sitei2199 – 22002Cleavage; by 3CLSPBy similarity
    Sitei2348 – 23492Cleavage; by 3CLSPBy similarity
    Sitei2458 – 24592Cleavage; by 3CLSPBy similarity
    Sitei3144 – 31452Cleavage; by 3CLSPBy similarity
    Sitei3195 – 31951Involved in mRNA transcription processBy similarity
    Sitei3585 – 35862Cleavage; by 3CLSPBy similarity
    Sitei3808 – 38092Cleavage; by 3CLSPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri8 – 2821C4-type; atypicalAdd
    BLAST
    Nucleotide bindingi3298 – 33058ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. helicase activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. RNA binding Source: InterPro
    6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    7. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    2. suppression by virus of host ISG15 activity Source: UniProtKB-KW
    3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
    4. suppression by virus of host STAT1 activity Source: UniProtKB-KW
    5. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    6. transcription, DNA-templated Source: InterPro
    7. viral protein processing Source: InterPro
    8. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Serine protease, Thiol protease, Transferase

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host STAT1 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1ab
    Alternative name(s):
    ORF1ab polyprotein
    Cleaved into the following 16 chains:
    Alternative name(s):
    PCP1-alpha
    Alternative name(s):
    PCP1-beta
    Alternative name(s):
    CP2
    Short name:
    CP
    Non-structural protein 3
    Short name:
    Nsp3
    3C-like serine proteinase (EC:3.4.21.-)
    Short name:
    3CLSP
    Alternative name(s):
    Nsp4
    Non-structural protein 5-6-7
    Short name:
    Nsp5-6-7
    Non-structural protein 5
    Short name:
    Nsp5
    Non-structural protein 6
    Short name:
    Nsp6
    Non-structural protein 7-alpha
    Short name:
    Nsp7-alpha
    Non-structural protein 7-beta
    Short name:
    Nsp7-beta
    Non-structural protein 8
    Short name:
    Nsp8
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Short name:
    Pol
    Short name:
    RdRp
    Alternative name(s):
    Nsp9
    Helicase (EC:3.6.4.12, EC:3.6.4.13)
    Short name:
    Hel
    Alternative name(s):
    Nsp10
    Non-structural protein 11
    Short name:
    Nsp11
    Non-structural protein 12
    Short name:
    Nsp12
    Gene namesi
    Name:rep
    ORF Names:1a-1b
    OrganismiPorcine reproductive and respiratory syndrome virus (strain 16244B) (PRRSV)
    Taxonomic identifieri300561 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesArteriviridaeArterivirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Chain Nsp1 : Host nucleus By similarity. Host cytoplasm By similarity

    GO - Cellular componenti

    1. host cell membrane Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Host nucleus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 39613961Replicase polyprotein 1abPRO_0000036657Add
    BLAST
    Chaini1 – 382382Nsp1By similarityPRO_0000410826Add
    BLAST
    Chaini1 – 180180Nsp1-alpha papain-like cysteine proteinaseBy similarityPRO_0000036659Add
    BLAST
    Chaini181 – 383203Nsp1-beta papain-like cysteine proteinaseBy similarityPRO_0000036660Add
    BLAST
    Chaini384 – 15791196Nsp2 cysteine proteinaseSequence AnalysisPRO_0000036661Add
    BLAST
    Chaini1580 – 1809230Non-structural protein 3By similarityPRO_0000036662Add
    BLAST
    Chaini1810 – 20132043C-like serine proteinaseBy similarityPRO_0000036663Add
    BLAST
    Chaini2014 – 2458445Non-structural protein 5-6-7By similarityPRO_0000036664Add
    BLAST
    Chaini2014 – 2183170Non-structural protein 5By similarityPRO_0000423118Add
    BLAST
    Chaini2184 – 219916Non-structural protein 6By similarityPRO_0000423119Add
    BLAST
    Chaini2200 – 2348149Non-structural protein 7-alphaBy similarityPRO_0000423120Add
    BLAST
    Chaini2349 – 2458110Non-structural protein 7-betaBy similarityPRO_0000423121Add
    BLAST
    Chaini2459 – 3144686RNA-directed RNA polymeraseBy similarityPRO_0000036665Add
    BLAST
    Chaini2459 – 250345Non-structural protein 8By similarityPRO_0000036666Add
    BLAST
    Chaini3145 – 3585441HelicaseBy similarityPRO_0000036667Add
    BLAST
    Chaini3586 – 3808223Non-structural protein 11By similarityPRO_0000036668Add
    BLAST
    Chaini3809 – 3961153Non-structural protein 12By similarityPRO_0000036669Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. Nsp1 is autocleaved into two subunits, Nsp1-alpha and Nsp1-beta. There are two alternative pathways for processing. Either nsp4-5 is cleaved, which represents the major pathway or the nsp5-6 and nsp6-7 are processed, which represents the minor pathway. The major pathway occurs when nsp2 acts as cofactor for nsp4 By similarity.By similarity

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1266 – 128621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1296 – 131621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1345 – 136521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1368 – 138821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1583 – 160321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1650 – 167021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1685 – 170521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1719 – 173921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2012 – 203221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2060 – 208021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2092 – 211221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2137 – 215721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2164 – 218421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini69 – 180112Peptidase C31PROSITE-ProRule annotationAdd
    BLAST
    Domaini263 – 383121Peptidase C32PROSITE-ProRule annotationAdd
    BLAST
    Domaini428 – 535108Peptidase C33PROSITE-ProRule annotationAdd
    BLAST
    Domaini1810 – 2013204Peptidase S32PROSITE-ProRule annotationAdd
    BLAST
    Domaini2890 – 3024135RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini3145 – 320864AV MBDAdd
    BLAST
    Domaini3265 – 3417153(+)RNA virus helicase ATP-bindingAdd
    BLAST
    Domaini3418 – 3546129(+)RNA virus helicase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 182114PCP1-alphaAdd
    BLAST
    Regioni263 – 382120PCP1-betaAdd
    BLAST
    Regioni426 – 51388OTU-likeAdd
    BLAST
    Regioni1266 – 1388123HD1Add
    BLAST
    Regioni1583 – 1745163HD2Add
    BLAST
    Regioni2036 – 2157122HD3Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi808 – 930123Pro-richAdd
    BLAST
    Compositional biasi2329 – 234416Pro-richAdd
    BLAST

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity
    The OTU-like region is responsible for the deubiquitinating and deISGylation activities of Nsp2.By similarity

    Sequence similaritiesi

    Belongs to the arteriviridae polyprotein family.Curated
    Contains 1 peptidase C31 domain.PROSITE-ProRule annotation
    Contains 1 peptidase C32 domain.PROSITE-ProRule annotation
    Contains 1 peptidase C33 domain.PROSITE-ProRule annotation
    Contains 1 peptidase S32 domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri8 – 2821C4-type; atypicalAdd
    BLAST

    Keywords - Domaini

    Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
    IPR008743. Arterivirus_Nsp2_C33.
    IPR023338. Arterivirus_NSP4_peptidase.
    IPR027355. AV_MBD_dom.
    IPR008741. AV_PCPalpha.
    IPR025773. AV_PCPbeta.
    IPR008760. EAV_peptidase_S32.
    IPR027417. P-loop_NTPase.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF05410. Peptidase_C31. 1 hit.
    PF05411. Peptidase_C32. 1 hit.
    PF05412. Peptidase_C33. 1 hit.
    PF05579. Peptidase_S32. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 2 hits.
    PROSITEiPS51538. AV_CP. 1 hit.
    PS51652. AV_MBD. 1 hit.
    PS51493. AV_NSP4_PRO. 1 hit.
    PS51539. AV_PCP_ALPHA. 1 hit.
    PS51540. AV_PCP_BETA. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform Replicase polyprotein 1ab (identifier: Q9YN02-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1ab

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSGILDRCTC TPNARVFVAE GQVYCTRCLS ARSLLPLNLQ VSELGVLGLF     50
    YRPEEPLRWT LPRAFPTVEC SPAGACWLSA IFPIARMTSG NLNFQQRMVR 100
    VAAEIYRAGQ LTPAVLKALQ VYERGCRWYP IVGPVPGVAV FANSLHVSDK 150
    PFPGATHVLT NLPLPQRPKP EDFCPFECAM ATVYDIGHDA VMYVAEGKIS 200
    WAPRGGDEVK FEAVPGELKL IANRLRTSFP PHHAVDMSKF AFTAPGCGVS 250
    MRVERQHGCL PADTVPEGNC WWSLFDLLPL EVQDKEIRHA NQFGYQTKHG 300
    VSGKYLQRRL QVNGLRAVTD SNGPIVVQYF SVKESWIRHL KLAGEPSYSG 350
    FEDLLRIRVE PNTSPLANTE GKIFRFGSHK WYGAGKRARK ARSCATATVA 400
    GRALSVRETR QAKEHEVAGA DKAEHLKHYS PPAEGNCGWH CISAIANRMV 450
    NSIFETTLPE RVRPPDDWAT DDDLANAIQI LRLPAALDRN GACTSAKYVL 500
    KLEGEHWTVT VTPGMSPSLL PLECVQGCCE HKGGLGSPDA IEVSGFDPAC 550
    LDWLAEVMHL PSSAIPAALA EMSGDSDRSA SPVTTVWTVS QFFARHSGGN 600
    HPDQVRLGKI ISLCQVIEDC CCSQNKTNRV TPEEVAAKID LYLRGATNLE 650
    ECLARLEKAR PPRVIDTSFD WDVVLPGVEA ATQTNKLPQV NQCRALVPVV 700
    TQKSLDNNSV PLTAFSLANY YYRAQGDEVR HRERLTAVLS KLEEVVREEY 750
    GLMPTEPGPR PTLPRGLDEL KDQMEEDLLR LANAQATSDM MAWAVEQVDL 800
    KTWVKNYPRW TPPPPPPKVQ PRKTKPVKSL PERKPVPAPR RKVGPDCGSP 850
    VSLGGDVPNS WEDLAVSSPL DLPTPPEPAT LSSELVIVSS PQCIFRPATP 900
    LSEPAPIPAP RGTVSRPVTP LSEPIPVPAP RRKFQQVKRL SSAAAVPLHQ 950
    NEPLDLSASS QTEYEASPSA PPQSGGVLGV EGHEAEETLS EISDMSGNIK 1000
    PASVSSSSSL SSVEITRPKY SAQAIIDSGG PCSGHLQGVK ETCLSVMREA 1050
    CDATKLDDPA TQEWLSRMWD RVDMLTWRNT SVCQAIRTLD GRLKFLPKMI 1100
    LETPPPYPCE FVMMPHTPAP SVGAESDLTI GSVATEDVPR ILEKIENVGE 1150
    MANQEPSAFS EDKPVDDQLV NDPRISSRRP DESTAAPSAG TGGAGSFTDL 1200
    PSSDGADADG GGPFRTAKRK AERLFDQLSR QVFDLVSHLP VFFSRLFHPG 1250
    GGYSTGDWGF AAFTLLCLFL CYSYPAFGIA PLLGVFSGTS RRVRMGVFGC 1300
    WLAFAVGLFK PVSDPVGAAC EFDSPECRNI LLSFELLKPW DPVRSLVVGP 1350
    VGLGLAILGR LLGGARCIWH FLLRLGIVAD CILAGAYVLS QGRCKKCWGS 1400
    CIRTAPNEVA FNVFPFTRAT RSSLIDLCDR FCAPKGMDPI FLATGWRGCW 1450
    AGRSPIEQPS EKPIAFAQLD EKKITARTVV AQPYDPNQAV KCLRVLQAGG 1500
    AMVAEAVPKV VKVSAVPFRA PFFPTGVKVD PDCRVVVDPD TFTAALRSGY 1550
    STTNLVLGVG DFAQLNGLKI RQISKPSGGG PHLMAALHVA CSMALHMLTG 1600
    IYVTAVGSCG TGTNDPWCAN PFAVPGYGPG SLCTSRLCIS QHGLTLPLTA 1650
    LVAGFGIQEI ALVVLIFVSI GGMAHRLSCK ADMLCILLAI ASYVWVPLTW 1700
    LLCVFPCWLR CFSLHPLTIL WLVFFLISVN MPSGILAMVL LVSLWLLGRY 1750
    TNVAGLVTPY DIHHYTSGPR GVAALATAPD GTYLAAVRRA ALTGRTMLFT 1800
    PSQLGSLLEG AFRTRKPSLN TVNVIGSSMG SGGVFTIDGK VKCVTAAHVL 1850
    TGNSARVSGV GFNQMLDFDV KGDFAIADCP NWQGAAPKAQ FCADGWTGRA 1900
    YWLTSSGVEP GVIGKGFAFC FTACGDSGSP VITEAGELVG VHTGSNKQGG 1950
    GIVTRPSGQF CNVAPIKLSE LSEFFAGPKV PLGDVKVGSH IIKDISEVPS 2000
    DLCALLAAKP ELEGGLSTVQ LLCVFFLLWR MMGHAWTPLV AVSFFILNEV 2050
    LPAVLVRSVF SFGMFVLSWL TPWSAQILMI RLLTAALNRN RWSLAFFSLG 2100
    AVTGFVADLA ATQGHPLQAV MNLSTYAFLP RMMVVTSPVP VITCGVVHLL 2150
    AIILYLFKYR GLHQILVGDG VFSAAFFLRY FAEGKLREGV SQSCGMNHES 2200
    LTGALAMRLN DEDLDFLMKW TDFKCFVSAS NMRNAAGQFI EAAYAKALRV 2250
    ELAQLVQVDK VRGVLAKLEA FADTVAPQLS PGDIVVALGH TPVGSIFDLK 2300
    VGSTKHTLQA IETRVLAGSK MTVARVVDPT PTPPPAPVPI PLPPKVLENG 2350
    PNAWGDEDRL NKKKRRRMEA LGIYVMGGKK YQKFWDKNSG DVFYEEVHNN 2400
    TDEWECLRVG DPADFDPEKG TLCGHVTIEN KAYHVYISPS GKKFLVPVNP 2450
    ENGRVQWEAA KLSMEQALGM MNVDGELTAK ELEKLKRIID KLQGLTKEQC 2500
    LNCLLAASGL TRCGRGGLVV TETAVKIVKF HNRTFTLGPV NLKVASEVEL 2550
    KDAVEHNQHP VARPIDGGVV LLRSAVPSLI DVLISGADAS PKLLAHHGPG 2600
    NTGIDGTLWD FESEATKEEV ALSAQIIQAC DIRRGDAPKI GLPYKLYPVR 2650
    GNPERVKGVL QNTRFGDIPY KTPSDTGSPV HAAACLTPNA TPVTDGRSVL 2700
    ATTMPPGFEL YVPTIPASVL DYLDSRPDCP KQLTEHGCED AALKDLSKYD 2750
    LSTQGFVLPG VLRLVRKYLF AHVGKCPPVH RPSTYPAKNS MAGINGNRFP 2800
    TKDIQSVPEI DVLCAQAVRE NWQTVTPCTL KKQYCGKKKT RTILGTNNFI 2850
    ALAHRAALSG VTQGFMKKAF NSPIALGKNK FKELQTSVLG RCLEADLASC 2900
    DRSTPAIVRW FAANLLYELA CAEEHLPSYV LNCCHDLLVT QSGAVTKRGG 2950
    LSSGDPITSV SNTIYSLVIY AQHMVLSYFK SGHPHGLLFL QDQLKFEDML 3000
    KVQPLIVYSD DLVLYAESPT MPNYHWWVEH LNLMLGFQTD PKKTAITDSP 3050
    SFLGCRIING RQLVPNRDRI LAALAYHMKA SNVSEYYASA AAILMDSCAC 3100
    LEYDPEWFEE LVVGIAQCAR KDGYSFPGTP FFMSMWEKLR SNYEGKKSRV 3150
    CGYCGAPAPY ATACGLDVCI YHTHFHQHCP VTIWCGHPAG SGSCSECKSP 3200
    VGKGTSPLDE VLEQVPYKPP RTVIMHVEQG LTPLDPGRYQ TRRGLVSVRR 3250
    GIRGNEVELP DGDYASTALL PTCKEINMVA VASNVLRSRF IIGPPGAGKT 3300
    YWLLQQVQDG DVIYTPTHQT MLDMIRALGT CRFNVPAGTT LQFPVPSRTG 3350
    PWVRILAGGW CPGKNSFLDE AAYCNHLDVL RLLSKTTLTC LGDFKQLHPV 3400
    GFDSHCYVFD IMPQTQLKTI WRFGQNICDA IQPDYRDKLM SMVNTTRVTY 3450
    VEKPVRYGQV LTPYHRDRED DAITIDSSQG ATFDVVTLHL PTKDSLNRQR 3500
    ALVAITRARH AIFVYDPHRQ LQGLFDLPAK GTPVNLAVHR DGQLIVLDRN 3550
    NKECTVAQAL GNGDKFRATD KRVVDSLRAI CADLEGSSSP LPKVAHNLGF 3600
    YFSPDLTQFA KLPVELAPHW PVVTTQNNEK WPDRLVASLR PIHKYSRACI 3650
    GAGYMVGPSV FLGTPGVVSY YLTKFVKGEA QLLPETVFST GRIEVDCREY 3700
    LDDREREVAA SLPHAFIGDV KGTTVGGCHH VTSRYLPRVL PKESVAVVGV 3750
    SSPGKAAKAL CTLTDVYLPD LEAYLHPETQ SKCWKMMLDF KEVRLMVWRD 3800
    KTAYFQLEGR YFTWYQLASY ASYIRVPVNS TVYLDPCMGP ALCNRRVVGS 3850
    THWGADLAVT PYDYGAKIIL SSAYHGEMPP GYKILACAEF SLDDPVRYKH 3900
    TWGFESDTAY LYEFTGNGED WEDYNDAFRA RQEGKIYKAT ATSLKFHFPP 3950
    GPVIEPTLGL N 3961

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:3,961
    Mass (Da):432,802
    Last modified:August 10, 2010 - v3
    Checksum:i111B73F5926C9DB0
    GO
    Isoform Replicase polyprotein 1a (identifier: Q9YN02-2) [UniParc]FASTAAdd to Basket

    Also known as: pp1a, ORF1a polyprotein

    The sequence of this isoform differs from the canonical sequence as follows:
         2504-3961: Missing.

    Note: Produced by conventional translation.

    Show »
    Length:2,503
    Mass (Da):271,602
    Checksum:i91175307A4EAB65C
    GO

    Sequence cautioni

    The sequence AAC64692.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2504 – 39611458Missing in isoform Replicase polyprotein 1a. CuratedVSP_032890Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF046869 Genomic RNA. Translation: AAC64691.1.
    AF046869 Genomic RNA. Translation: AAC64692.1. Different initiation.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF046869 Genomic RNA. Translation: AAC64691.1 .
    AF046869 Genomic RNA. Translation: AAC64692.1 . Different initiation.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
    IPR008743. Arterivirus_Nsp2_C33.
    IPR023338. Arterivirus_NSP4_peptidase.
    IPR027355. AV_MBD_dom.
    IPR008741. AV_PCPalpha.
    IPR025773. AV_PCPbeta.
    IPR008760. EAV_peptidase_S32.
    IPR027417. P-loop_NTPase.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF05410. Peptidase_C31. 1 hit.
    PF05411. Peptidase_C32. 1 hit.
    PF05412. Peptidase_C33. 1 hit.
    PF05579. Peptidase_S32. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 2 hits.
    PROSITEi PS51538. AV_CP. 1 hit.
    PS51652. AV_MBD. 1 hit.
    PS51493. AV_NSP4_PRO. 1 hit.
    PS51539. AV_PCP_ALPHA. 1 hit.
    PS51540. AV_PCP_BETA. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions."
      Allende R., Lewis T.L., Lu Z., Rock D.L., Kutish G.F., Ali A., Doster A.R., Osorio F.A.
      J. Gen. Virol. 80:307-315(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiRPOA_PRRS1
    AccessioniPrimary (citable) accession number: Q9YN02
    Secondary accession number(s): Q9YN01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 98 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3