Q9YKL3 (Q9YKL3_9FLAV) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Polyprotein EMBL AAD18036.1
Organism	Dengue virus 2 EMBL AAD18036.1
Taxonomic identifier	11060 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Flavivirus › Dengue virus group › Dengue virus

Protein attributes

Sequence length	3391 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes By similarity. SAAS SAAS011998
Catalytic activity	ATP + H2O = ADP + phosphate. SAAS SAAS007094 NTP + H2O = NDP + phosphate. SAAS SAAS007094 Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SAAS SAAS007094
Subcellular location	Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein By similarity SAAS SAAS011998.
Sequence similarities	Contains 1 RdRp catalytic domain. SAAS SAAS007094 Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SAAS SAAS007094

Ontologies

Keywords
Biological process	Clathrin-mediated endocytosis of virus by host SAAS SAAS011998 Fusion of virus membrane with host endosomal membrane SAAS SAAS011998 Fusion of virus membrane with host membrane Host-virus interaction Viral RNA replication SAAS SAAS007094 Viral attachment to host cell SAAS SAAS011998 Viral penetration into host cytoplasm Virus endocytosis by host Virus entry into host cell
Cellular component	Host endoplasmic reticulum SAAS SAAS011998 Host membrane SAAS SAAS011998 Membrane Viral envelope protein SAAS SAAS011998 Virion
Domain	Transmembrane Transmembrane helix SAAS SAAS011998
Ligand	ATP-binding Nucleotide-binding
Molecular function	Capsid protein SAAS SAAS011998 Helicase SAAS SAAS007094 Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase SAAS SAAS007094 Serine protease SAAS SAAS013754 Transferase
PTM	Disulfide bond SAAS SAAS011998
Technical term	3D-structure PDB 2M0S
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Inferred from electronic annotation. Source: GOC RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC proteolysis Inferred from electronic annotation. Source: UniProtKB-KW viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell via clathrin-mediated endocytosis Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral capsid Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro mRNA (nucleoside-2'-O-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q9YKL3 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 9116552CBE254A70	FASTA	3,391	379,439
	10 20 30 40 50 60 MNNQRKKARS TPFNMLKRER NRVSTVQQLT KRFSLGMLQG RGPLKLFMAL VAFLRFLTIP 70 80 90 100 110 120 PTAGILKRWG TIKKSKAINV LRGFRKEIGR MLNILNRRRR TVGVIIMLIP TAMAFHLTTR 130 140 150 160 170 180 NGEPHMIVGR QEKGKSLLFK TEDGVNMCTL MAIDLGELCE DTITYKCPLL RQNEPEDIDC 190 200 210 220 230 240 WCNSTSTWVT YGTCTTTGEH RREKRSVALV PHVGMGLETR TETWMSSEGA WKHAQRIETW 250 260 270 280 290 300 ILRHPGFIIM AAILAYTIGT THFQRALIFI LLTAVAPSMT MRCIGVSNRD FVEGVSGGSW 310 320 330 340 350 360 VDIVLEHGSC VTTMAKNKPT LDFELIKTEA KQPVTLRKYC IKAKLTNTTT ESRCPTQGEP 370 380 390 400 410 420 SLNEEQDKRF VCKHSMVDRG WGNGCGLFGK GGIVTCATFT CKKNMEGKIV QPENLEYTIV 430 440 450 460 470 480 ITPHSGEEHA VGNDTGKHGK EIKITPQSSI TEAELTGYGT VTMECSPRTG LDFNEMVLLQ 490 500 510 520 530 540 MEDKAWLVHR QWFLDLPLPW LPGADTQGSN WIQKETLVTF KNPHAKKQDV VVLGSQEGAM 550 560 570 580 590 600 HTALTGATEI QMSSGNLLFT GHLKCRLRMD KLQLKGMSYS MCTGKFKIVK EIAETQHGTI 610 620 630 640 650 660 VIRVQYEGDG SPCKIPFEIM DLEKRHVLGR LITVNPIVTE KDSPVNIEAE PPFGDSYIII 670 680 690 700 710 720 GVEPGQLKLN WVKKGSSIGQ MFETTMRGAK RMAILGDTAW DFGSLGGVFT SIGKALHQVF 730 740 750 760 770 780 GAIYGAAFSG VSWTMKILIG VIITWIGMNS RSTSLSVSLV LVGVITLYLG AMVQADSGCV 790 800 810 820 830 840 VSWKNKELKC GSGIFITDNV HTWTEQYKFQ PESPSKLASA IQKAHEEGIC GIRSVTRLEN 850 860 870 880 890 900 LMWKQITPEL NHILSENEVK LTIMTGDIKG IMQAGKRSLR PQPTELKYSW KTWGKAKMLS 910 920 930 940 950 960 TESHNQTFLI DGPETAECPN TNRAWNSLEV EDYGFGVFTT NIWLKLREKQ DVFCDSKLMS 970 980 990 1000 1010 1020 AAIKDNRAVH ADMGYWIESA LNDTWKMEKA SFIEVKSCHW PKSHTLWSNG VLESEMIIPK 1030 1040 1050 1060 1070 1080 SFAGPVSQHN YRPGYYTQTA GPWHLGKLEM DFDFCEGTTV VVTEDCGNRG PSLRTTTASG 1090 1100 1110 1120 1130 1140 KLITEWCCRS CTLPPLRYRG EDGCWYGMEI RPLKEKEESL VDSLVTAGHG QIDNFSLGVL 1150 1160 1170 1180 1190 1200 GMALFLEEML RTRVGTKHAI LLVAVSFVTL ITGNMSLRDL GRVMVMVGAT MTDDIGMGVT 1210 1220 1230 1240 1250 1260 YLALLAAFKV RPTFAAGLLL RKLTSKELMM ATIGIALLSQ STIPETILEL TDALALGMMV 1270 1280 1290 1300 1310 1320 LKIVRNMEKY QLAVTIMAIS CVPNAVILQN AWKVSCTILA AVSVSPLLLT SSQQKADWIP 1330 1340 1350 1360 1370 1380 LALTIKGLNP TAIFLTTLAR TSKKRSWPLN EAIMAVGMVS ILASSLLKND IPMTGPLVAG 1390 1400 1410 1420 1430 1440 GLLTVCYVLT GRSADLELER ATDVKWEDQA EISGSSPILS ITISEDGSMS IKNEEEEQTL 1450 1460 1470 1480 1490 1500 TILIRTGLLV ISGLFPVSIP ITAAAWYLWE VKKQRAGVLW DVPSPPPVEK AELEDGAYRI 1510 1520 1530 1540 1550 1560 KQKGILGYSQ IGTGVYKEGT FHTMWHVTRG AVLMHRGKRI EPSWADVKKD LISYGGGWKL 1570 1580 1590 1600 1610 1620 EGEWKEGEEV QVLALEPGKN PRAVQTKPGL FKTNTGTIGA VSLDFSPGTS GSPIVDRKGK 1630 1640 1650 1660 1670 1680 VVGLYGNGVV TRSGAYVSAI AQTEKSIEDN PEIEDDIFRK KRLTIMDLHP GAGKTKRYLP 1690 1700 1710 1720 1730 1740 AIVREAIKRG LRTLILAPTR VVAAEMEEAL RGLPIRYQTP AIRAEHTGRE IVDLMCHATF 1750 1760 1770 1780 1790 1800 TMRLLSPVRV PNYNLIIMDE AHFTDPANIA ARGYISTRVE MGEAAGIFMT ATPPGSRDPF 1810 1820 1830 1840 1850 1860 PQSNAPIMDE EREIPERSWN SGHEWVTDFK GKTVWFVPSI KAGNDIAACL RKNGKKVIQL 1870 1880 1890 1900 1910 1920 SRKTFDSEYV KTRTNDWDFV VTTDISEMGA NFKAERVIDP RRCMKPVILT DGEERVILAG 1930 1940 1950 1960 1970 1980 PMPVTHSSAA QRRGRIGRNP KNENDQYIYM GEPLENDEDC AHWKEAKMLL DNINTPEGII 1990 2000 2010 2020 2030 2040 PSMFEPEREK VDAIDGEYRL RGEARKTFVD LMRRGDLPVW LAYRVAAEGI NYADRRWCFD 2050 2060 2070 2080 2090 2100 GVKNNQILEE NVEVEIWTKE GERKKLKPRW LDARIYSDPL ALKEFKEFAA GRKSLTLNLI 2110 2120 2130 2140 2150 2160 TEMGRLPTFM TQKARDALDN LAVLHTAEAG GRAYNHALSE LPETLETLLL LTLLATVTGG 2170 2180 2190 2200 2210 2220 IFLFLMSGKG VGKMTLGMCC IITASILLWY AQIQPHWIAA SIILEFFLMV LLIPEPEKQR 2230 2240 2250 2260 2270 2280 TPQDNQLTYV VIAILTVVAA TMANEMGFLE KTKKDLGLGS IATQEPESNI LDIDLRPASA 2290 2300 2310 2320 2330 2340 WTLYAVATTF VTPMLRHSIE NSSVNVSLTA IANQATVLMG LGKGWPLSKM DIGVPLLAIG 2350 2360 2370 2380 2390 2400 CYSQVNPITL TAALLLLVAH YAIIGPGLQA KATREAQKRA AAGIMKNPTV DGITVIDLDP 2410 2420 2430 2440 2450 2460 IPYDPKFEKQ LGQVMLLILC VTQVLMMRTT WALCEALTLA TGPISTLWEG NPGRFWNTTI 2470 2480 2490 2500 2510 2520 AVSMANIFRG SYLAGAGLLF FIMKNTTNTR RGTGNIGETL GEKWKSRLNA LGKSEFQIYK 2530 2540 2550 2560 2570 2580 KSGIQEVDRT LAKEGIKRGE TDHHAVSRGS AKLRWFVERN MVTPEGKVVD LGCGRGGWSY 2590 2600 2610 2620 2630 2640 YCGGLKNVRE VKGLTKGGPG HEEPIPMSTY GWNLVRLQSG VDVFFTPPEK CDTLLCDIGE 2650 2660 2670 2680 2690 2700 SSPNPTIEAG RTLRVLSLVE NWLNNDTQFC IKVLNPYMPS VIEKMETLQR KYGGALVRNP 2710 2720 2730 2740 2750 2760 LSRNSTHEMY WVSNATGNIV SSVNMISRML INRFTMKHKK ATYEPDVDLG SGTRNIGIES 2770 2780 2790 2800 2810 2820 EIPNLDIIGK RIEKIKQEHE TSWHYDQDHP YKTWAYHGSY ETKQTGSASS MVNGVVRLLT 2830 2840 2850 2860 2870 2880 KPWDVVPMVT QMAMTDTTPF GQQRVFKEKV DTRTQEPKEG TKKLMKITAE WLWKELGKKK 2890 2900 2910 2920 2930 2940 TPRMCTREEF TRKVRSNAAL GAIFTDENKW KSAREAVEDN RFWELVDKER NLHLEGKCET 2950 2960 2970 2980 2990 3000 CVYNMMGKRE KKLGEFGKAK GSRAIWYMWL GARFLEFEAL GFLNEDHWFS RENSLSGVEG 3010 3020 3030 3040 3050 3060 EGLHKLGYIL RDVSKKEGGA MYADDTAGWD TRITLEDLKN EEMVTNHMEG EHKKLAEAIF 3070 3080 3090 3100 3110 3120 KLTYQNKAVR VQRPTPRGTV MDIISRRDQR GSGQVGTYGL NTFTNMEAQL IRQMEGEGIF 3130 3140 3150 3160 3170 3180 KSIQHLTVTE EIAVQNWLAR VGRERLSRMA ISGDDCVVKP LDDRFASALT ALNDMGKVRK 3190 3200 3210 3220 3230 3240 DIQQWEPSRG WNDWTQVPFC SHHFHELIMK DGRVLVVPCR NQDELIGRAR ISQGAGWSLK 3250 3260 3270 3280 3290 3300 ETACLGKSYA QMWSLMYFHR RDLRLAANAI CSAVPSHWVP TSRTTWSIHA KHEWMTTEDM 3310 3320 3330 3340 3350 3360 LTVWNRVWIQ ENPWMEDKTP VESWEEVPYL GKREDQWCGS LIGLTSRATW AKNIQTAINQ 3370 3380 3390 VRSLIGNEEY TDYMPSMKRF RREEEEAGVL W

References

[1]	"Cloning and sequencing of the complete genome of dengue type 2 virus (04) isolated from a Chinese patient with dengue hemorrhagic fever." Yang J., Yang P.Y., Hu Z.J., Qin E.D., Yu M., Ou W., Zhou Y.S. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: China 04 EMBL AAD18036.1.
[2]	"Membrane topology and function of dengue virus NS2A protein." Xie X., Gayen S., Kang C., Yuan Z., Shi P.Y. J. Virol. 0:0-0(2013) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1194-1221.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF119661 Genomic RNA. Translation: AAD18036.1.

PIR

B25817.
JS0219.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2M0S	NMR	-	A	1194-1221	[»]

HSSP

HSSP built from PDB template 1OKE based on UniProtKB Q88653.

ProteinModelPortal

Q9YKL3.

SMR

Q9YKL3. Positions 21-100, 115-195, 281-674, 1394-1440, 1495-2093, 2498-2759, 2765-3374.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

Gene3D

2.60.40.350. 1 hit.
2.60.98.10. 2 hits.
3.30.387.10. 1 hit.

InterPro

IPR011492. DEAD_Flavivir.
IPR000069. Env_glycoprot_M_flavivir.
IPR013755. Flav_gly_cen_dom_subdom1.
IPR001122. Flavi_capsidC.
IPR026470. Flavi_E_Stem/Anchor_dom.
IPR001157. Flavi_NS1.
IPR000752. Flavi_NS2A.
IPR000487. Flavi_NS2B.
IPR000404. Flavi_NS4A.
IPR001528. Flavi_NS4B.
IPR002535. Flavi_propep.
IPR000336. Flavivir/Alphavir_Ig-like.
IPR001850. Flavivirus_NS3_S7.
IPR027287. Flavovir_Ig-like.
IPR014412. Gen_Poly_FLV.
IPR011998. Glycoprot_cen/dimer.
IPR013754. GlyE_dim.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR026490. mRNA_cap_0/1_MeTrfase.
IPR000208. RNA-dir_pol_flavivirus.
IPR007094. RNA-dir_pol_PSvirus.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]

Pfam

PF01003. Flavi_capsid. 1 hit.
PF07652. Flavi_DEAD. 1 hit.
PF02832. Flavi_glycop_C. 1 hit.
PF00869. Flavi_glycoprot. 1 hit.
PF01004. Flavi_M. 1 hit.
PF00948. Flavi_NS1. 1 hit.
PF01005. Flavi_NS2A. 1 hit.
PF01002. Flavi_NS2B. 1 hit.
PF01350. Flavi_NS4A. 1 hit.
PF01349. Flavi_NS4B. 1 hit.
PF00972. Flavi_NS5. 1 hit.
PF01570. Flavi_propep. 1 hit.
PF01728. FtsJ. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00949. Peptidase_S7. 1 hit.
[Graphical view]

PIRSF

PIRSF003817. Gen_Poly_FLV. 1 hit.

SMART

SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]

SUPFAM

SSF56983. Flavi_glycoprotE. 1 hit.
SSF81296. Ig_E-set. 1 hit.
SSF50494. Pept_Ser_Cys. 1 hit.

TIGRFAMs

TIGR04240. flavi_E_stem. 1 hit.

PROSITE

PS51527. FLAVIVIRUS_NS2B. 1 hit.
PS51528. FLAVIVIRUS_NS3PRO. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51591. RNA_CAP01_NS5_MT. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

Q9YKL3_9FLAV

Accession

Primary (citable) accession number: Q9YKL3

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1999
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)