SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9YID8

- POLG_HPE23

UniProt

Q9YID8 - POLG_HPE23

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Genome polyprotein

Gene
N/A
Organism
Human parechovirus 2 (strain CT86-6760) (HPeV-2) (Echovirus 23)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Protein 2A: Is not a protease By similarity.1 Publication
Protein 2B: Affects membrane integrity and cause an increase in membrane permeability By similarity.1 Publication
Protein 2C: Associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities By similarity.1 Publication
Protein 3A, via its hydrophobic domain, serves as membrane anchor By similarity.1 Publication
Protease 3C: cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind cooperatively to the protease By similarity.1 Publication
RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals By similarity.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein.
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
NTP + H2O = NDP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei784 – 7852Cleavage; by protease 2A Reviewed prediction
Active sitei1558 – 15581For protease 3C activity Reviewed prediction
Active sitei1589 – 15891For protease 3C activity Reviewed prediction
Active sitei1678 – 16781For protease 3C activity Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1193 – 12008ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. ion channel activity Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW
  5. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  6. RNA helicase activity Source: InterPro
  7. structural molecule activity Source: InterPro

GO - Biological processi

  1. induction by virus of host autophagy Source: UniProtKB
  2. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  3. protein oligomerization Source: UniProtKB-KW
  4. RNA-protein covalent cross-linking Source: UniProtKB-KW
  5. suppression by virus of host RIG-I activity by RIG-I proteolysis Source: UniProtKB
  6. suppression by virus of host translation initiation factor activity Source: UniProtKB
  7. transcription, DNA-templated Source: InterPro
  8. viral entry into host cell Source: UniProtKB-KW
  9. viral RNA genome replication Source: InterPro
  10. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase, Viral ion channel

Keywords - Biological processi

Host-virus interaction, Ion transport, Transport, Viral attachment to host cell, Viral RNA replication, Virus entry into host cell

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 10 chains:
Alternative name(s):
P1AB
Virion protein 0
Alternative name(s):
P1C
Virion protein 3
Alternative name(s):
P1D
Virion protein 1
Protein 2A
Short name:
P2A
Protein 2B
Short name:
P2B
Protein 2C (EC:3.6.1.15)
Short name:
P2C
Protein 3A
Short name:
P3A
Protein 3B
Short name:
P3B
Alternative name(s):
VPg
Protease 3C (EC:3.4.22.28)
Short name:
P3C
Alternative name(s):
Picornain 3C
OrganismiHuman parechovirus 2 (strain CT86-6760) (HPeV-2) (Echovirus 23)
Taxonomic identifieri122961 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeParechovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008274: Genome

Subcellular locationi

Chain Protein VP3 : Virion By similarity. Host cytoplasm Reviewed prediction
Chain Protein VP1 : Virion By similarity. Host cytoplasm Reviewed prediction
Chain Protein 2B : Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Reviewed prediction
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.
Chain Protein 2C : Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Reviewed prediction
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.
Chain Protein 3A : Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Reviewed prediction
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.
Chain Protein 3B : Virion Reviewed prediction
Chain Protease 3C : Host cytoplasm Reviewed prediction
Chain RNA-directed RNA polymerase 3D-POL : Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Reviewed prediction
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.

GO - Cellular componenti

  1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  2. integral to membrane of host cell Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. viral capsid Source: UniProtKB-KW
  5. viral genome Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Host cytoplasmic vesicle, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Protein VP0 Reviewed predictionPRO_0000039740Add
BLAST
Chaini291 – 549259Protein VP3 Reviewed predictionPRO_0000039741Add
BLAST
Chaini550 – 784235Protein VP1 Reviewed predictionPRO_0000039742Add
BLAST
Chaini785 – 931147Protein 2A Reviewed predictionPRO_0000039743Add
BLAST
Chaini932 – 1053122Protein 2B Reviewed predictionPRO_0000039744Add
BLAST
Chaini1054 – 1382329Protein 2C Reviewed predictionPRO_0000039745Add
BLAST
Chaini1383 – 1499117Protein 3A Reviewed predictionPRO_0000039747Add
BLAST
Chaini1500 – 151920Protein 3B By similarityPRO_0000311174Add
BLAST
Chaini1520 – 1719200Protease 3C Reviewed predictionPRO_0000039748Add
BLAST
Chaini1720 – 2188469RNA-directed RNA polymerase 3D-POL Reviewed predictionPRO_0000039749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1502 – 15021O-(5'-phospho-RNA)-tyrosine By similarity

Post-translational modificationi

VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.
Specific enzymatic cleavages yield mature proteins. All cleavages are catalyzed by P3C.

Keywords - PTMi

Covalent protein-RNA linkage, Lipoprotein, Myristate, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ9YID8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1165 – 1326162SF3 helicaseAdd
BLAST
Domaini1953 – 2067115RdRp catalyticAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi772 – 7743Cell attachment site Reviewed prediction

Sequence similaritiesi

Contains 2 peptidase C3 domains.

Family and domain databases

Gene3Di2.60.120.20. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
IPR009419. VPP_parechovir_P3A.
IPR009407. VPP_parechovir_P3B.
[Graphical view]
PfamiPF06344. Parecho_VpG. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF06363. Picorna_P3A. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 2 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YID8-1 [UniParc]FASTAAdd to Basket

« Hide

METIKSIADM ATGFTNTIDS TVNAVTEGVS KIGNDSGGEI LTKVADDASN     50
LLGPNCVAST SQPENKDVVQ ATTTVNTLTN LTQHPSAPTM PFTPDFSNVD 100
VFHSMAYDIT TGDKNPSKLI RLDTTTWQHT WPRQHLINDV ELPKAFWDKN 150
SKPAYGQSRY FAAVRCGFHF QVQINVNQGT AGCALVVYEP KPIVTHGGHL 200
EFGSYTNLPH VLMNLAETTQ ADLCIPYVSD TNYVKTDSSD LGRLRVYVWT 250
PLTIPSSATN DVDVTVLGSL LQLDFQNPRT YDTDVNIYDN SPLDTKTKYG 300
KLRFSKKILS MSTKYKWTRN KIDIAEGPGS MNMANVLSTT GAQSIALVGE 350
RAFYDPRTAG SKSRFGDMIH IAQLFSVMSD TTTPSTSSGI DDLGYLDWSA 400
TYVPQQVIHR NVVKLNQFSN LKPFVNAYTY FRGSLVLRMS VYASTFNRGR 450
LRMGFFPNFT TNTTSEMDNA IYTICDIGSD NSFEITIPYT FSTWMRKTNG 500
RPIGLFQVEV LNRLTYNSSC PNKVHCIVQG RLGNDARFYC PTGSLVEFQN 550
SWGSQMDLTD PLCVEDDEAE DCKQTISPDE LGLTSAQDDG PLGVEKPNYF 600
LNFRAINVDI FTVSHTKVDN IFGRAWLALE HTFADDGTWR ADLNFPTQGH 650
GTLTRLFTYY SGELNVHVLY LSDNGFLRVT HAYDHDNDRS NFLSSNGVIT 700
VPAGEQMTLS VPFYSSKPLR TIRETGALGK LICKPLLSGT HSGKIEVYLS 750
LRCPNLFFPS PAPKEKTSRA LRGDLANFID QSPYGQQQQT QMMKLAYLDR 800
GFYKHYGIIV GGYVYQLDSD DIFKTALTGK ARFTKTRLTP DWIVEEECEL 850
DYFRVKYLES SVNSEHIFSV DSNCETIAKD IFGTHTLSQH QAIGLVGAIL 900
LTAGLMSTIK TPVNATTIKE FFNHAIDGDE QGLSLLVQKC TTFFSSAATE 950
ILDNDLVKFI VKILVRILCY MVLYCHKPNI LTTACLSTLL IMDVTSSSVL 1000
SPSCKALMQC LMDGDVKKLA EVVAESMSNT DDDEIKEQIC DTVKYTKTIL 1050
SNQGPFKGFN EVSTAFRHVD WWIHTLLKIK DMVLSVFKPS IESKAIQWLE 1100
RNKEHVCSIL DYASDIIVES KDQTKMKTQE FYQRYSDCLA KFKPIMAICF 1150
RSCHNSISNT VYRLFQELAR IPNRISTQND LIRVEPIGVW IQGEPGQGKS 1200
FLTHTLSRQL QKSCKLNGVY TNPTASEFMD GYDNQDIHLI DDLGQTRKEK 1250
DIEMLCNCIS SVPFIVPMAH LEEKGKFYTS KLVIATTNKS DFSSTVLQDS 1300
GALKRRFPYI MHIRAAKAYS KSGKLNVSQA MSTMSTGECW EVSKNGRDWE 1350
TLKLKDLVQK ITEDYQERQK NYNAWKQQLE NQTLDDLDDA VSYIKHNFPD 1400
AIPYIDEYLN IEMSTLIEQM EAFIEPRPSV FKCFAVKLPH KPGKQPRKLW 1450
AGSAGKIKSM LSFIERNKAW LTVVSAVTSA ISILLLVTKI FKKEESKDER 1500
AYNPTLPITK PKGTFPVSQR EFKNEAPYDG QLEHIISQMA YITGSTTGHL 1550
THCAGYQHDE IILHGHSIKY LEQEEDLTLH YKNKVFPIEN PSVTQVTLGG 1600
KPMDLAILKC KLPFRFKKNS KYYTNKIGTE SMLIWMTEQG IITKEVQRVH 1650
HSGGIKTREG TESTKTISYT VKSCKGMCGG LLISKVEGNF KILGMHIAGN 1700
GEMGVAIPFN FLKNDMSDQG IITEVTPIQP MYINTKSQIH KSPVYGAVEV 1750
KMGPAVLSKS DTRLEEPVDC LIKKSASKYR VNKFQVNNEL WQGVKACVKS 1800
KFREIFGVNG IVDMKTAILG TSHVNSMDLS TSAGYSLVKS GYKKKDLICL 1850
EPFSVSPMLE KLVQDKFHNL LKGNQITTIF NTCLKDELRK LDKIAAGKTR 1900
CIEACEVDYC IVYRMIMMEI YDKIYQTPCY YSGLAVGINP YKDWHFMINA 1950
LNDYNYEMDY SQYDGSLSSM LLWEAVEVLA YCHDSPDLVM QLHKPVIDSD 2000
HVVFNERWLI HGGMPSGSPC TTVLNSLCNL MMCIYTTNLI SPGVDCLPIV 2050
YGDDVILSLD REIEPERLQS IMADSFGAEV TGSRKDEPPS LKPRMEVEFL 2100
KRKPGYFPES TFIVGKLDTE NMIQHLMWMK NFSTFKQQLQ SYLMELCLHG 2150
KDIYQRYIKI LDPYLKEWNI VVDDYDVVIA KLMPMVFD 2188
Length:2,188
Mass (Da):246,604
Last modified:May 1, 1999 - v1
Checksum:i02CC77D0A5ED3D93
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF055846 Genomic RNA. Translation: AAC79756.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF055846 Genomic RNA. Translation: AAC79756.1 .

3D structure databases

ProteinModelPortali Q9YID8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.20. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
IPR009419. VPP_parechovir_P3A.
IPR009407. VPP_parechovir_P3B.
[Graphical view ]
Pfami PF06344. Parecho_VpG. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF06363. Picorna_P3A. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 2 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00918. CALICVIRUSNS.
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of echovirus 23 and its relationship to echovirus 22 and other human enteroviruses."
    Oberste M.S., Maher K., Pallansch M.A.
    Virus Res. 56:217-223(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Molecular analysis of human parechovirus type 2 (formerly echovirus 23)."
    Ghazi F., Hughes P.J., Hyypiae T., Stanway G.
    J. Gen. Virol. 79:2641-2650(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PROTEIN 2A.

Entry informationi

Entry nameiPOLG_HPE23
AccessioniPrimary (citable) accession number: Q9YID8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi