Q9YI92 (SLB_GLOBL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Snaclec mamushigin subunit beta
Organism	Gloydius blomhoffii (Mamushi) (Agkistrodon halys blomhoffi)
Taxonomic identifier	242054 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Crotalinae › Gloydius

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to platelet glycoprotein Ibalpha (GP1BA) and enhances platelet aggregation at low-shear stress. At high-shear stress, blocks platelet aggregation in a dose-dependent manner.
Subunit structure	Heterodimer of subunits alpha and beta; disulfide-linked. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the snaclec family. Contains 1 C-type lectin domain.
Mass spectrometry	Molecular mass is 15413±6 Da from positions 24 - 146. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hemostasis impairing toxin Platelet aggregation activating toxin Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	blood coagulation Inferred from direct assay PubMed 98319530. Source: UniProtKB
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 146	123	Snaclec mamushigin subunit beta		PRO_0000017527
Regions
	Domain	32 – 143	112	C-type lectin
Amino acid modifications
	Disulfide bond	25 ↔ 36		By similarity UniProtKB P22029
	Disulfide bond	53 ↔ 142		By similarity UniProtKB P22029
	Disulfide bond	98		Interchain (with C-103 in subunit alpha) By similarity UniProtKB P22029
	Disulfide bond	119 ↔ 134		By similarity UniProtKB P22029

Sequences

	Sequence		Length	Mass (Da)
	Q9YI92 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 9EDA84BDCC24E76D	FASTA	146	17,064
	10 20 30 40 50 60 MGRFIFLSFG LLVVFVSLSG TGADCPSDWS SYEGHCYRVF QKEMTWEDAE KFCTQQRKES 70 80 90 100 110 120 HLVSFHSSEE VDFVVSMTWP ILKYDFVWIG LNNIWNECMV EWTDGTRLSH NAWITESECI 130 140 AAKTTDNQWL SRPCSRTYNV VCKFQE

References

[1]

"The cDNA cloning and molecular characterization of a snake venom platelet glycoprotein Ib-binding protein, mamushigin, from Agkistrodon halys blomhoffii venom." Sakurai Y., Fujimura Y., Kokubo T., Imamura K., Kawasaki T., Handa M., Suzuki M., Matsui T., Titani K., Yoshioka A. Thromb. Haemost. 79:1199-1207(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-56, SUBUNIT, MASS SPECTROMETRY. Tissue: Venom and Venom gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB019616 mRNA. Translation: BAA34425.1.
3D structure databases
ProteinModelPortal	Q9YI92.
SMR	Q9YI92. Positions 25-145.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG004151.
Family and domain databases
Gene3D	3.10.100.10. 1 hit.
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR016187. C-type_lectin_fold. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SLB_GLOBL

Accession

Primary (citable) accession number: Q9YI92

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	May 1, 1999
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Annotation program

Animal Toxin Annotation Program