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Protein

5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene

alas2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase (alas1), 5-aminolevulinate synthase, erythroid-specific, mitochondrial (alas2), 5-aminolevulinate synthase (alas1), 5-aminolevulinate synthase (alas2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei158SubstrateBy similarity1
Binding sitei275SubstrateBy similarity1
Binding sitei327Pyridoxal phosphateBy similarity1
Binding sitei355Pyridoxal phosphateBy similarity1
Binding sitei383Pyridoxal phosphateBy similarity1
Active sitei386By similarity1
Binding sitei415Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei416Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei503SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • embryonic hemopoiesis Source: ZFIN
  • erythrocyte development Source: ZFIN
  • erythrocyte differentiation Source: UniProtKB
  • heme biosynthetic process Source: UniProtKB
  • hemoglobin biosynthetic process Source: UniProtKB
  • hemoglobin metabolic process Source: ZFIN
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  • response to hypoxia Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-DRE-189451. Heme biosynthesis.
UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-E
Alternative name(s):
5-aminolevulinic acid synthase 2
Delta-ALA synthase 2
Delta-aminolevulinate synthase 2
Gene namesi
Name:alas2
Synonyms:alas-e
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-001229-1. alas2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000001227? – 5835-aminolevulinate synthase, erythroid-specific, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei386N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9YHT4.

Expressioni

Gene expression databases

BgeeiENSDARG00000038643.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000056419.

Structurei

3D structure databases

ProteinModelPortaliQ9YHT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1360. Eukaryota.
COG0156. LUCA.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiQ9YHT4.
KOiK00643.
PhylomeDBiQ9YHT4.
TreeFamiTF300724.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YHT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFLHHCPF LKSSPGPSAR KVATYLNLAD RCPIIVRQIS AKAAQSSEQN
60 70 80 90 100
GLLPHKEPKR QLATTATQVA VSMSQSCPFV SSKIGLVKAS PQVQEDVQPN
110 120 130 140 150
LENQDTSGLI SSLFSGLQSH QSTGPTHLLQ DNFNRPTFSY DEFFTQKIVE
160 170 180 190 200
KKKDHTYRIF KTVNRFAEVF PFAEDYSIAG RLGSQVSVWC SNDYLGMSRH
210 220 230 240 250
PRVVKAIGDA LKKHGAGAGG TRNISGTSNY HVALENELAR LHQKDGALVF
260 270 280 290 300
SSCFVANDST LFTLAKMLPG CEIYSDMGNH ASMIQGIRNS GAKRFIFRHN
310 320 330 340 350
DASHLEELLS RSDPLTPKIV AFETVHSMDG AICPLEELCD VAHKYGALTF
360 370 380 390 400
VDEVHAVGLY GAHGAGVGER DNVMHKIDIV SGTLGKAFGC VGGYIASTAA
410 420 430 440 450
LVDTVRSFAA GFIFTTSLPP MVLAGALESV RVLKSDEGQA LRRAHQRNVK
460 470 480 490 500
HMRQLLLDAG LPVVNCPSHI IPIRVGNAAK NSEVCDILLE KHNIYVQAIN
510 520 530 540 550
YPTVPRGEEL LRLAPSPFHN PIMMNYFAEK LLDVWQEVGL PLNGPAQASC
560 570 580
TFCDRPLHFD LMSEWEKSYF GNMEPRYITV AAQ
Length:583
Mass (Da):63,909
Last modified:May 1, 1999 - v1
Checksum:i9C542B4C75299ABD
GO

Sequence cautioni

The sequence AAH67149 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095747 mRNA. Translation: AAC72835.1.
BC056338 mRNA. Translation: AAH56338.1.
BC067149 mRNA. Translation: AAH67149.1. Different initiation.
RefSeqiNP_571757.1. NM_131682.2.
UniGeneiDr.8180.

Genome annotation databases

EnsembliENSDART00000056420; ENSDARP00000056419; ENSDARG00000038643.
GeneIDi64607.
KEGGidre:64607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095747 mRNA. Translation: AAC72835.1.
BC056338 mRNA. Translation: AAH56338.1.
BC067149 mRNA. Translation: AAH67149.1. Different initiation.
RefSeqiNP_571757.1. NM_131682.2.
UniGeneiDr.8180.

3D structure databases

ProteinModelPortaliQ9YHT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000056419.

Proteomic databases

PaxDbiQ9YHT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000056420; ENSDARP00000056419; ENSDARG00000038643.
GeneIDi64607.
KEGGidre:64607.

Organism-specific databases

CTDi212.
ZFINiZDB-GENE-001229-1. alas2.

Phylogenomic databases

eggNOGiKOG1360. Eukaryota.
COG0156. LUCA.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiQ9YHT4.
KOiK00643.
PhylomeDBiQ9YHT4.
TreeFamiTF300724.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.
ReactomeiR-DRE-189451. Heme biosynthesis.

Miscellaneous databases

PROiQ9YHT4.

Gene expression databases

BgeeiENSDARG00000038643.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM0_DANRE
AccessioniPrimary (citable) accession number: Q9YHT4
Secondary accession number(s): Q6NXC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.