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Q9YHT4

- HEM0_DANRE

UniProt

Q9YHT4 - HEM0_DANRE

Protein

5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene

alas2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei158 – 1581SubstrateBy similarity
    Binding sitei275 – 2751SubstrateBy similarity
    Binding sitei327 – 3271Pyridoxal phosphateBy similarity
    Binding sitei355 – 3551Pyridoxal phosphateBy similarity
    Binding sitei383 – 3831Pyridoxal phosphateBy similarity
    Active sitei386 – 3861By similarity
    Binding sitei415 – 4151Pyridoxal phosphateBy similarity
    Binding sitei416 – 4161Pyridoxal phosphateBy similarity
    Binding sitei503 – 5031SubstrateBy similarity

    GO - Molecular functioni

    1. 5-aminolevulinate synthase activity Source: UniProtKB
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. embryonic hemopoiesis Source: ZFIN
    2. erythrocyte differentiation Source: UniProtKB
    3. heme biosynthetic process Source: UniProtKB
    4. hemoglobin biosynthetic process Source: UniProtKB
    5. hemoglobin metabolic process Source: ZFIN
    6. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
    7. response to hypoxia Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Heme biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00375.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
    Short name:
    ALAS-E
    Alternative name(s):
    5-aminolevulinic acid synthase 2
    Delta-ALA synthase 2
    Delta-aminolevulinate synthase 2
    Gene namesi
    Name:alas2
    Synonyms:alas-e
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 8

    Organism-specific databases

    ZFINiZDB-GENE-001229-1. alas2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB
    2. mitochondrial matrix Source: UniProtKB-SubCell
    3. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 5835-aminolevulinate synthase, erythroid-specific, mitochondrialPRO_0000001227
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei386 – 3861N6-(pyridoxal phosphate)lysineBy similarity

    Expressioni

    Gene expression databases

    BgeeiQ9YHT4.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9YHT4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0156.
    GeneTreeiENSGT00530000063111.
    HOGENOMiHOG000221020.
    HOVERGENiHBG005954.
    InParanoidiQ9YHT4.
    KOiK00643.
    OMAiHYLQSIN.
    OrthoDBiEOG7TQV0F.
    PhylomeDBiQ9YHT4.
    TreeFamiTF300724.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR015118. 5aminolev_synth_preseq.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    PF09029. Preseq_ALAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9YHT4-1 [UniParc]FASTAAdd to Basket

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    MSAFLHHCPF LKSSPGPSAR KVATYLNLAD RCPIIVRQIS AKAAQSSEQN    50
    GLLPHKEPKR QLATTATQVA VSMSQSCPFV SSKIGLVKAS PQVQEDVQPN 100
    LENQDTSGLI SSLFSGLQSH QSTGPTHLLQ DNFNRPTFSY DEFFTQKIVE 150
    KKKDHTYRIF KTVNRFAEVF PFAEDYSIAG RLGSQVSVWC SNDYLGMSRH 200
    PRVVKAIGDA LKKHGAGAGG TRNISGTSNY HVALENELAR LHQKDGALVF 250
    SSCFVANDST LFTLAKMLPG CEIYSDMGNH ASMIQGIRNS GAKRFIFRHN 300
    DASHLEELLS RSDPLTPKIV AFETVHSMDG AICPLEELCD VAHKYGALTF 350
    VDEVHAVGLY GAHGAGVGER DNVMHKIDIV SGTLGKAFGC VGGYIASTAA 400
    LVDTVRSFAA GFIFTTSLPP MVLAGALESV RVLKSDEGQA LRRAHQRNVK 450
    HMRQLLLDAG LPVVNCPSHI IPIRVGNAAK NSEVCDILLE KHNIYVQAIN 500
    YPTVPRGEEL LRLAPSPFHN PIMMNYFAEK LLDVWQEVGL PLNGPAQASC 550
    TFCDRPLHFD LMSEWEKSYF GNMEPRYITV AAQ 583
    Length:583
    Mass (Da):63,909
    Last modified:May 1, 1999 - v1
    Checksum:i9C542B4C75299ABD
    GO

    Sequence cautioni

    The sequence AAH67149.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095747 mRNA. Translation: AAC72835.1.
    BC056338 mRNA. Translation: AAH56338.1.
    BC067149 mRNA. Translation: AAH67149.1. Different initiation.
    RefSeqiNP_571757.1. NM_131682.2.
    UniGeneiDr.8180.

    Genome annotation databases

    EnsembliENSDART00000056420; ENSDARP00000056419; ENSDARG00000038643.
    GeneIDi64607.
    KEGGidre:64607.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095747 mRNA. Translation: AAC72835.1 .
    BC056338 mRNA. Translation: AAH56338.1 .
    BC067149 mRNA. Translation: AAH67149.1 . Different initiation.
    RefSeqi NP_571757.1. NM_131682.2.
    UniGenei Dr.8180.

    3D structure databases

    ProteinModelPortali Q9YHT4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000056420 ; ENSDARP00000056419 ; ENSDARG00000038643 .
    GeneIDi 64607.
    KEGGi dre:64607.

    Organism-specific databases

    CTDi 212.
    ZFINi ZDB-GENE-001229-1. alas2.

    Phylogenomic databases

    eggNOGi COG0156.
    GeneTreei ENSGT00530000063111.
    HOGENOMi HOG000221020.
    HOVERGENi HBG005954.
    InParanoidi Q9YHT4.
    KOi K00643.
    OMAi HYLQSIN.
    OrthoDBi EOG7TQV0F.
    PhylomeDBi Q9YHT4.
    TreeFami TF300724.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00375 .

    Miscellaneous databases

    NextBioi 20901975.
    PROi Q9YHT4.

    Gene expression databases

    Bgeei Q9YHT4.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR015118. 5aminolev_synth_preseq.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    PF09029. Preseq_ALAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemia."
      Brownlie A., Donovan A., Pratt S.J., Paw B.H., Oates A.C., Brugnara C., Witkowska H.E., Sassa S., Zon L.I.
      Nat. Genet. 20:244-250(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiHEM0_DANRE
    AccessioniPrimary (citable) accession number: Q9YHT4
    Secondary accession number(s): Q6NXC5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3