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Q9YHT4

- HEM0_DANRE

UniProt

Q9YHT4 - HEM0_DANRE

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Protein
5-aminolevulinate synthase, erythroid-specific, mitochondrial
Gene
alas2, alas-e
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pyridoxal phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei158 – 1581Substrate By similarity
Binding sitei275 – 2751Substrate By similarity
Binding sitei327 – 3271Pyridoxal phosphate By similarity
Binding sitei355 – 3551Pyridoxal phosphate By similarity
Binding sitei383 – 3831Pyridoxal phosphate By similarity
Active sitei386 – 3861 By similarity
Binding sitei415 – 4151Pyridoxal phosphate By similarity
Binding sitei416 – 4161Pyridoxal phosphate By similarity
Binding sitei503 – 5031Substrate By similarity

GO - Molecular functioni

  1. 5-aminolevulinate synthase activity Source: UniProtKB
  2. pyridoxal phosphate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. embryonic hemopoiesis Source: ZFIN
  2. erythrocyte differentiation Source: UniProtKB
  3. heme biosynthetic process Source: UniProtKB
  4. hemoglobin biosynthetic process Source: UniProtKB
  5. hemoglobin metabolic process Source: ZFIN
  6. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  7. response to hypoxia Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-E
Alternative name(s):
5-aminolevulinic acid synthase 2
Delta-ALA synthase 2
Delta-aminolevulinate synthase 2
Gene namesi
Name:alas2
Synonyms:alas-e
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-001229-1. alas2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial matrix Source: UniProtKB-SubCell
  3. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 5835-aminolevulinate synthase, erythroid-specific, mitochondrialPRO_0000001227
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei386 – 3861N6-(pyridoxal phosphate)lysine By similarity

Expressioni

Gene expression databases

BgeeiQ9YHT4.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9YHT4.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0156.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiQ9YHT4.
KOiK00643.
OMAiHYLQSIN.
OrthoDBiEOG7TQV0F.
PhylomeDBiQ9YHT4.
TreeFamiTF300724.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YHT4-1 [UniParc]FASTAAdd to Basket

« Hide

MSAFLHHCPF LKSSPGPSAR KVATYLNLAD RCPIIVRQIS AKAAQSSEQN    50
GLLPHKEPKR QLATTATQVA VSMSQSCPFV SSKIGLVKAS PQVQEDVQPN 100
LENQDTSGLI SSLFSGLQSH QSTGPTHLLQ DNFNRPTFSY DEFFTQKIVE 150
KKKDHTYRIF KTVNRFAEVF PFAEDYSIAG RLGSQVSVWC SNDYLGMSRH 200
PRVVKAIGDA LKKHGAGAGG TRNISGTSNY HVALENELAR LHQKDGALVF 250
SSCFVANDST LFTLAKMLPG CEIYSDMGNH ASMIQGIRNS GAKRFIFRHN 300
DASHLEELLS RSDPLTPKIV AFETVHSMDG AICPLEELCD VAHKYGALTF 350
VDEVHAVGLY GAHGAGVGER DNVMHKIDIV SGTLGKAFGC VGGYIASTAA 400
LVDTVRSFAA GFIFTTSLPP MVLAGALESV RVLKSDEGQA LRRAHQRNVK 450
HMRQLLLDAG LPVVNCPSHI IPIRVGNAAK NSEVCDILLE KHNIYVQAIN 500
YPTVPRGEEL LRLAPSPFHN PIMMNYFAEK LLDVWQEVGL PLNGPAQASC 550
TFCDRPLHFD LMSEWEKSYF GNMEPRYITV AAQ 583
Length:583
Mass (Da):63,909
Last modified:May 1, 1999 - v1
Checksum:i9C542B4C75299ABD
GO

Sequence cautioni

The sequence AAH67149.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095747 mRNA. Translation: AAC72835.1.
BC056338 mRNA. Translation: AAH56338.1.
BC067149 mRNA. Translation: AAH67149.1. Different initiation.
RefSeqiNP_571757.1. NM_131682.2.
UniGeneiDr.8180.

Genome annotation databases

EnsembliENSDART00000056420; ENSDARP00000056419; ENSDARG00000038643.
GeneIDi64607.
KEGGidre:64607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095747 mRNA. Translation: AAC72835.1 .
BC056338 mRNA. Translation: AAH56338.1 .
BC067149 mRNA. Translation: AAH67149.1 . Different initiation.
RefSeqi NP_571757.1. NM_131682.2.
UniGenei Dr.8180.

3D structure databases

ProteinModelPortali Q9YHT4.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000056420 ; ENSDARP00000056419 ; ENSDARG00000038643 .
GeneIDi 64607.
KEGGi dre:64607.

Organism-specific databases

CTDi 212.
ZFINi ZDB-GENE-001229-1. alas2.

Phylogenomic databases

eggNOGi COG0156.
GeneTreei ENSGT00530000063111.
HOGENOMi HOG000221020.
HOVERGENi HBG005954.
InParanoidi Q9YHT4.
KOi K00643.
OMAi HYLQSIN.
OrthoDBi EOG7TQV0F.
PhylomeDBi Q9YHT4.
TreeFami TF300724.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00375 .

Miscellaneous databases

NextBioi 20901975.
PROi Q9YHT4.

Gene expression databases

Bgeei Q9YHT4.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemia."
    Brownlie A., Donovan A., Pratt S.J., Paw B.H., Oates A.C., Brugnara C., Witkowska H.E., Sassa S., Zon L.I.
    Nat. Genet. 20:244-250(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiHEM0_DANRE
AccessioniPrimary (citable) accession number: Q9YHT4
Secondary accession number(s): Q6NXC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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