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Protein

5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene

alas2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei158 – 1581SubstrateBy similarity
Binding sitei275 – 2751SubstrateBy similarity
Binding sitei327 – 3271Pyridoxal phosphateBy similarity
Binding sitei355 – 3551Pyridoxal phosphateBy similarity
Binding sitei383 – 3831Pyridoxal phosphateBy similarity
Active sitei386 – 3861By similarity
Binding sitei415 – 4151Pyridoxal phosphateBy similarity
Binding sitei416 – 4161Pyridoxal phosphateBy similarity
Binding sitei503 – 5031SubstrateBy similarity

GO - Molecular functioni

  1. 5-aminolevulinate synthase activity Source: UniProtKB
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. embryonic hemopoiesis Source: ZFIN
  2. erythrocyte differentiation Source: UniProtKB
  3. heme biosynthetic process Source: UniProtKB
  4. hemoglobin biosynthetic process Source: UniProtKB
  5. hemoglobin metabolic process Source: ZFIN
  6. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  7. response to hypoxia Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_280746. Heme biosynthesis.
UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-E
Alternative name(s):
5-aminolevulinic acid synthase 2
Delta-ALA synthase 2
Delta-aminolevulinate synthase 2
Gene namesi
Name:alas2
Synonyms:alas-e
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-001229-1. alas2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial matrix Source: UniProtKB-SubCell
  3. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 5835-aminolevulinate synthase, erythroid-specific, mitochondrialPRO_0000001227
Transit peptidei1 – ?MitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei386 – 3861N6-(pyridoxal phosphate)lysineBy similarity

Expressioni

Gene expression databases

BgeeiQ9YHT4.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9YHT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0156.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiQ9YHT4.
KOiK00643.
OMAiRAEKYIY.
OrthoDBiEOG7TQV0F.
PhylomeDBiQ9YHT4.
TreeFamiTF300724.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YHT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFLHHCPF LKSSPGPSAR KVATYLNLAD RCPIIVRQIS AKAAQSSEQN
60 70 80 90 100
GLLPHKEPKR QLATTATQVA VSMSQSCPFV SSKIGLVKAS PQVQEDVQPN
110 120 130 140 150
LENQDTSGLI SSLFSGLQSH QSTGPTHLLQ DNFNRPTFSY DEFFTQKIVE
160 170 180 190 200
KKKDHTYRIF KTVNRFAEVF PFAEDYSIAG RLGSQVSVWC SNDYLGMSRH
210 220 230 240 250
PRVVKAIGDA LKKHGAGAGG TRNISGTSNY HVALENELAR LHQKDGALVF
260 270 280 290 300
SSCFVANDST LFTLAKMLPG CEIYSDMGNH ASMIQGIRNS GAKRFIFRHN
310 320 330 340 350
DASHLEELLS RSDPLTPKIV AFETVHSMDG AICPLEELCD VAHKYGALTF
360 370 380 390 400
VDEVHAVGLY GAHGAGVGER DNVMHKIDIV SGTLGKAFGC VGGYIASTAA
410 420 430 440 450
LVDTVRSFAA GFIFTTSLPP MVLAGALESV RVLKSDEGQA LRRAHQRNVK
460 470 480 490 500
HMRQLLLDAG LPVVNCPSHI IPIRVGNAAK NSEVCDILLE KHNIYVQAIN
510 520 530 540 550
YPTVPRGEEL LRLAPSPFHN PIMMNYFAEK LLDVWQEVGL PLNGPAQASC
560 570 580
TFCDRPLHFD LMSEWEKSYF GNMEPRYITV AAQ
Length:583
Mass (Da):63,909
Last modified:April 30, 1999 - v1
Checksum:i9C542B4C75299ABD
GO

Sequence cautioni

The sequence AAH67149.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095747 mRNA. Translation: AAC72835.1.
BC056338 mRNA. Translation: AAH56338.1.
BC067149 mRNA. Translation: AAH67149.1. Different initiation.
RefSeqiNP_571757.1. NM_131682.2.
UniGeneiDr.8180.

Genome annotation databases

EnsembliENSDART00000056420; ENSDARP00000056419; ENSDARG00000038643.
GeneIDi64607.
KEGGidre:64607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095747 mRNA. Translation: AAC72835.1.
BC056338 mRNA. Translation: AAH56338.1.
BC067149 mRNA. Translation: AAH67149.1. Different initiation.
RefSeqiNP_571757.1. NM_131682.2.
UniGeneiDr.8180.

3D structure databases

ProteinModelPortaliQ9YHT4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000056420; ENSDARP00000056419; ENSDARG00000038643.
GeneIDi64607.
KEGGidre:64607.

Organism-specific databases

CTDi212.
ZFINiZDB-GENE-001229-1. alas2.

Phylogenomic databases

eggNOGiCOG0156.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiQ9YHT4.
KOiK00643.
OMAiRAEKYIY.
OrthoDBiEOG7TQV0F.
PhylomeDBiQ9YHT4.
TreeFamiTF300724.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.
ReactomeiREACT_280746. Heme biosynthesis.

Miscellaneous databases

NextBioi20901975.
PROiQ9YHT4.

Gene expression databases

BgeeiQ9YHT4.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemia."
    Brownlie A., Donovan A., Pratt S.J., Paw B.H., Oates A.C., Brugnara C., Witkowska H.E., Sassa S., Zon L.I.
    Nat. Genet. 20:244-250(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiHEM0_DANRE
AccessioniPrimary (citable) accession number: Q9YHT4
Secondary accession number(s): Q6NXC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 22, 2002
Last sequence update: April 30, 1999
Last modified: March 31, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.