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Protein

5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene

alas2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase (alas1), 5-aminolevulinate synthase, erythroid-specific, mitochondrial (alas2), 5-aminolevulinate synthase (alas1), 5-aminolevulinate synthase (alas2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei158SubstrateBy similarity1
Binding sitei275SubstrateBy similarity1
Binding sitei327Pyridoxal phosphateBy similarity1
Binding sitei355Pyridoxal phosphateBy similarity1
Binding sitei383Pyridoxal phosphateBy similarity1
Active sitei386By similarity1
Binding sitei415Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei416Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei503SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • embryonic hemopoiesis Source: ZFIN
  • erythrocyte development Source: ZFIN
  • erythrocyte differentiation Source: UniProtKB
  • heme biosynthetic process Source: ZFIN
  • hemoglobin biosynthetic process Source: ZFIN
  • hemoglobin metabolic process Source: ZFIN
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  • response to hypoxia Source: UniProtKB

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processHeme biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-DRE-189451 Heme biosynthesis
UniPathwayiUPA00251; UER00375

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-E
Alternative name(s):
5-aminolevulinic acid synthase 2
Delta-ALA synthase 2
Delta-aminolevulinate synthase 2
Gene namesi
Name:alas2
Synonyms:alas-e
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 8

Organism-specific databases

ZFINiZDB-GENE-001229-1 alas2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000001227? – 5835-aminolevulinate synthase, erythroid-specific, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei386N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9YHT4
PRIDEiQ9YHT4

Expressioni

Gene expression databases

BgeeiENSDARG00000038643
ExpressionAtlasiQ9YHT4 baseline

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000056419

Structurei

3D structure databases

ProteinModelPortaliQ9YHT4
SMRiQ9YHT4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1360 Eukaryota
COG0156 LUCA
GeneTreeiENSGT00530000063111
HOGENOMiHOG000221020
HOVERGENiHBG005954
InParanoidiQ9YHT4
KOiK00643
OMAiKQDHTYR
PhylomeDBiQ9YHT4
TreeFamiTF300724

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR010961 4pyrrol_synth_NH2levulA_synth
IPR015118 5aminolev_synth_preseq
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PF09029 Preseq_ALAS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01821 5aminolev_synth, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YHT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFLHHCPF LKSSPGPSAR KVATYLNLAD RCPIIVRQIS AKAAQSSEQN
60 70 80 90 100
GLLPHKEPKR QLATTATQVA VSMSQSCPFV SSKIGLVKAS PQVQEDVQPN
110 120 130 140 150
LENQDTSGLI SSLFSGLQSH QSTGPTHLLQ DNFNRPTFSY DEFFTQKIVE
160 170 180 190 200
KKKDHTYRIF KTVNRFAEVF PFAEDYSIAG RLGSQVSVWC SNDYLGMSRH
210 220 230 240 250
PRVVKAIGDA LKKHGAGAGG TRNISGTSNY HVALENELAR LHQKDGALVF
260 270 280 290 300
SSCFVANDST LFTLAKMLPG CEIYSDMGNH ASMIQGIRNS GAKRFIFRHN
310 320 330 340 350
DASHLEELLS RSDPLTPKIV AFETVHSMDG AICPLEELCD VAHKYGALTF
360 370 380 390 400
VDEVHAVGLY GAHGAGVGER DNVMHKIDIV SGTLGKAFGC VGGYIASTAA
410 420 430 440 450
LVDTVRSFAA GFIFTTSLPP MVLAGALESV RVLKSDEGQA LRRAHQRNVK
460 470 480 490 500
HMRQLLLDAG LPVVNCPSHI IPIRVGNAAK NSEVCDILLE KHNIYVQAIN
510 520 530 540 550
YPTVPRGEEL LRLAPSPFHN PIMMNYFAEK LLDVWQEVGL PLNGPAQASC
560 570 580
TFCDRPLHFD LMSEWEKSYF GNMEPRYITV AAQ
Length:583
Mass (Da):63,909
Last modified:May 1, 1999 - v1
Checksum:i9C542B4C75299ABD
GO

Sequence cautioni

The sequence AAH67149 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095747 mRNA Translation: AAC72835.1
BC056338 mRNA Translation: AAH56338.1
BC067149 mRNA Translation: AAH67149.1 Different initiation.
RefSeqiNP_571757.1, NM_131682.2
UniGeneiDr.8180

Genome annotation databases

EnsembliENSDART00000056420; ENSDARP00000056419; ENSDARG00000038643
GeneIDi64607
KEGGidre:64607

Similar proteinsi

Entry informationi

Entry nameiHEM0_DANRE
AccessioniPrimary (citable) accession number: Q9YHT4
Secondary accession number(s): Q6NXC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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