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Q9YHT2

- SDHB_CHICK

UniProt

Q9YHT2 - SDHB_CHICK

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Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gene

SDHB

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Iron-sulfur 1 (2Fe-2S)
Metal bindingi108 – 1081Iron-sulfur 1 (2Fe-2S)
Metal bindingi111 – 1111Iron-sulfur 1 (2Fe-2S)
Metal bindingi123 – 1231Iron-sulfur 1 (2Fe-2S)
Metal bindingi196 – 1961Iron-sulfur 2 (4Fe-4S)
Metal bindingi199 – 1991Iron-sulfur 2 (4Fe-4S)
Metal bindingi202 – 2021Iron-sulfur 2 (4Fe-4S)
Metal bindingi206 – 2061Iron-sulfur 3 (3Fe-4S)
Binding sitei211 – 2111Ubiquinone; shared with DHSD2 Publications
Metal bindingi253 – 2531Iron-sulfur 3 (3Fe-4S)
Metal bindingi259 – 2591Iron-sulfur 3 (3Fe-4S)
Metal bindingi263 – 2631Iron-sulfur 2 (4Fe-4S)

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
  2. 3 iron, 4 sulfur cluster binding Source: UniProtKB
  3. 4 iron, 4 sulfur cluster binding Source: UniProtKB
  4. electron carrier activity Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
  6. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  7. ubiquinone binding Source: UniProtKB

GO - Biological processi

  1. small molecule metabolic process Source: Reactome
  2. tricarboxylic acid cycle Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_115536. The tricarboxylic acid cycle.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Iron-sulfur subunit of complex II
Short name:
Ip
Gene namesi
Name:SDHB
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Mitochondrion inner membrane 3 Publications; Peripheral membrane protein 3 Publications; Matrix side 3 Publications

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial respiratory chain complex II Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838MitochondrionAdd
BLAST
Chaini39 – 290252Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrialPRO_0000343800Add
BLAST

Proteomic databases

PaxDbiQ9YHT2.
PRIDEiQ9YHT2.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD.3 Publications

Protein-protein interaction databases

STRINGi9031.ENSGALP00000000693.

Structurei

Secondary structure

1
290
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi49 – 568Combined sources
Beta strandi67 – 748Combined sources
Helixi75 – 773Combined sources
Helixi82 – 9211Combined sources
Beta strandi104 – 1085Combined sources
Beta strandi112 – 1154Combined sources
Beta strandi118 – 1214Combined sources
Helixi122 – 1243Combined sources
Beta strandi135 – 1384Combined sources
Beta strandi140 – 1423Combined sources
Beta strandi144 – 1474Combined sources
Helixi154 – 1629Combined sources
Turni173 – 1764Combined sources
Helixi184 – 1885Combined sources
Turni189 – 1957Combined sources
Helixi203 – 2053Combined sources
Helixi207 – 2126Combined sources
Turni213 – 2153Combined sources
Helixi218 – 22912Combined sources
Helixi237 – 2426Combined sources
Turni247 – 2526Combined sources
Helixi258 – 2625Combined sources
Helixi269 – 28214Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YQ3X-ray2.20B39-290[»]
1YQ4X-ray2.33B39-290[»]
2FBWX-ray2.10B/O39-290[»]
2H88X-ray1.74B/O39-290[»]
2H89X-ray2.40B39-290[»]
2WQYX-ray2.10B/O39-290[»]
ProteinModelPortaliQ9YHT2.
SMRiQ9YHT2. Positions 44-285.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9YHT2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 143942Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini186 – 216314Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
HOVERGENiHBG005483.
InParanoidiQ9YHT2.
PhylomeDBiQ9YHT2.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YHT2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAVVGVSL RRGVPARFLR AGLRPVRGLE AVHGICRGAQ TAAAATSRIK
60 70 80 90 100
KFSIYRWDPD KPGDKPRMQT YEVDLNKCGP MVLDALIKIK NELDSTLTFR
110 120 130 140 150
RSCREGICGS CAMNIAGGNT LACTKKIDPD LSKTTKIYPL PHMYVVKDLV
160 170 180 190 200
PDLSNFYAQY KSIEPYLKKK DESKQGKEQY LQSIEDRQKL DGLYECILCA
210 220 230 240 250
CCSTSCPSYW WNGDKYLGPA VLMQAYRWMI DSRDDYTEER LAQLQDPFSL
260 270 280 290
YRCHTIMNCT RTCPKGLNPG KAIAEIKKMM ATYKEKAAAA
Length:290
Mass (Da):32,597
Last modified:May 1, 1999 - v1
Checksum:i313E698866B3FDFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095937 mRNA. Translation: AAC72372.1.
UniGeneiGga.4743.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095937 mRNA. Translation: AAC72372.1 .
UniGenei Gga.4743.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1YQ3 X-ray 2.20 B 39-290 [» ]
1YQ4 X-ray 2.33 B 39-290 [» ]
2FBW X-ray 2.10 B/O 39-290 [» ]
2H88 X-ray 1.74 B/O 39-290 [» ]
2H89 X-ray 2.40 B 39-290 [» ]
2WQY X-ray 2.10 B/O 39-290 [» ]
ProteinModelPortali Q9YHT2.
SMRi Q9YHT2. Positions 44-285.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000000693.

Proteomic databases

PaxDbi Q9YHT2.
PRIDEi Q9YHT2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0479.
HOGENOMi HOG000160590.
HOVERGENi HBG005483.
InParanoidi Q9YHT2.
PhylomeDBi Q9YHT2.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01006 .
Reactomei REACT_115536. The tricarboxylic acid cycle.

Miscellaneous databases

EvolutionaryTracei Q9YHT2.
NextBioi 20920287.
PROi Q9YHT2.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view ]
Pfami PF13085. Fer2_3. 1 hit.
[Graphical view ]
SUPFAMi SSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR00384. dhsB. 1 hit.
PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "OXPHOS genes in mammals and the molecular clock."
    Weinreich D.M.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  2. "Crystallization of mitochondrial respiratory complex II from chicken heart: a membrane-protein complex diffracting to 2.0 A."
    Huang L.-S., Borders T.M., Shen J.T., Wang C.-J., Berry E.A.
    Acta Crystallogr. D 61:380-387(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF OF 39-290, SUBUNIT, SUBCELLULAR LOCATION.
  3. "Crystallographic studies of the binding of ligands to the dicarboxylate site of complex II, and the identity of the ligand in the 'oxaloacetate-inhibited' state."
    Huang L.-S., Shen J.T., Wang A.C., Berry E.A.
    Biochim. Biophys. Acta 1757:1073-1083(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) OF OF 39-290 IN COMPLEX WITH UBIQUINONE AND IRON-SULFUR CENTERS, SUBUNIT, SUBCELLULAR LOCATION.
  4. "3-nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme."
    Huang L.-S., Sun G., Cobessi D., Wang A.C., Shen J.T., Tung E.Y., Anderson V.E., Berry E.A.
    J. Biol. Chem. 281:5965-5972(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 39-290 IN COMPLEX WITH UBIQUINONE AND IRON-SULFUR CENTERS, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSDHB_CHICK
AccessioniPrimary (citable) accession number: Q9YHT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3