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Q9YH85

- TECTA_CHICK

UniProt

Q9YH85 - TECTA_CHICK

Protein

Alpha-tectorin

Gene

TECTA

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    One of the major non-collagenous components of the tectorial membrane. The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals.1 Publication

    GO - Biological processi

    1. cell-matrix adhesion Source: InterPro
    2. sensory perception of sound Source: UniProtKB-KW

    Keywords - Biological processi

    Hearing

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16938.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-tectorin
    Gene namesi
    Name:TECTA
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Cell membrane Curated; Lipid-anchorGPI-anchor Curated; Extracellular side Curated. Secretedextracellular spaceextracellular matrix
    Note: Found in the non-collagenous matrix of the tectorial membrane.By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    3. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Extracellular matrix, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 20582034Alpha-tectorinPRO_0000041739Add
    BLAST
    Propeptidei2059 – 212062Removed in mature formSequence AnalysisPRO_0000041740Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi258 – 2581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi496 – 4961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi666 – 6661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi792 – 7921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi822 – 8221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi834 – 8341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi877 – 8771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi899 – 8991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi907 – 9071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi928 – 9281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1025 – 10251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1041 – 10411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1207 – 12071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1337 – 13371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1511 – 15111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1537 – 15371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1723 – 17231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1739 – 17391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1761 – 17611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1818 – 18181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1831 – 18311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1847 – 18471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1887 – 18871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1906 – 19061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1947 ↔ 2007PROSITE-ProRule annotation
    Lipidationi2058 – 20581GPI-anchor amidated asparagineSequence Analysis

    Post-translational modificationi

    At least 3 products of tectorin seem to exist: HMM, MMM and LMM. They may be generated by active processing or the result of proteolysis occurring between intrachain disulfide bonds.
    The presence of a hydrophobic C-terminus preceded by a potential cleavage site strongly suggests that tectorins are synthesized as glycosylphosphatidylinositol-linked, membrane-bound precursors. Tectorins are targeted to the apical surface of the inner ear epithelia by the lipid and proteolytically released into the extracellular compartment.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ9YH85.

    Expressioni

    Tissue specificityi

    Expressed in the inner ear.1 Publication

    Developmental stagei

    Apically located within the epithelium of the developing basilar papilla at days E5.5 to E8. As development proceeds, expression becomes restricted to the basal layer. In the utricle, alpha-tectorin is first expressed at E4.5.1 Publication

    Interactioni

    Subunit structurei

    May form homomeric filament after self-association or heteromeric filament after association with beta-tectorin.

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000010663.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9YH85.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini98 – 252155NIDOPROSITE-ProRule annotationAdd
    BLAST
    Domaini260 – 31253VWFCAdd
    BLAST
    Domaini318 – 520203VWFD 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini578 – 62043TIL 1Add
    BLAST
    Domaini691 – 908218VWFD 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini963 – 101351TIL 2Add
    BLAST
    Domaini1067 – 1289223VWFD 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini1345 – 139854TIL 3Add
    BLAST
    Domaini1459 – 1674216VWFD 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini1772 – 2026255ZPPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2091 – 20944Poly-Ser

    Domaini

    Zona pellucida domain may enable to form filaments.

    Sequence similaritiesi

    Contains 1 NIDO domain.PROSITE-ProRule annotation
    Contains 1 VWFC domain.Curated
    Contains 4 VWFD domains.PROSITE-ProRule annotation
    Contains 1 ZP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG317991.
    HOVERGENiHBG079244.
    KOiK18273.
    PhylomeDBiQ9YH85.

    Family and domain databases

    InterProiIPR000742. EG-like_dom.
    IPR003886. Nidogen_extracell_dom.
    IPR002919. TIL_dom.
    IPR025615. TILa_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR006552. VWC_out.
    IPR001846. VWF_type-D.
    IPR001507. ZP_dom.
    IPR017977. ZP_dom_CS.
    [Graphical view]
    PfamiPF08742. C8. 3 hits.
    PF06119. NIDO. 1 hit.
    PF01826. TIL. 3 hits.
    PF12714. TILa. 2 hits.
    PF00094. VWD. 4 hits.
    PF00100. Zona_pellucida. 1 hit.
    [Graphical view]
    SMARTiSM00832. C8. 3 hits.
    SM00181. EGF. 1 hit.
    SM00539. NIDO. 1 hit.
    SM00215. VWC_out. 1 hit.
    SM00216. VWD. 4 hits.
    SM00241. ZP. 1 hit.
    [Graphical view]
    SUPFAMiSSF57567. SSF57567. 3 hits.
    PROSITEiPS51220. NIDO. 1 hit.
    PS51233. VWFD. 4 hits.
    PS00682. ZP_1. 1 hit.
    PS51034. ZP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9YH85-1 [UniParc]FASTAAdd to Basket

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    MNTRSLLSAW AALLVVTVRH RAHAMASLYP FWPNDTKTPK VDDGSSSEIK     50
    LSVPFIFFRS PYRTVYVNNN GVISFNSLVS QFTPEAFPLA DGRAFVAPFC 100
    GDVANGIRGE IYYRESTNPE LLGESSKDIR KYFKDMASFS ASWVFIVTWE 150
    EVQFYGGSST TPVNTFQAVL ITDGVSSFAI FNYQEISWTT GTASGGDPLT 200
    GLGGVMAQAG FNGGNISNFF SIPGSRTPDI VNIEQTTNVN IPGRWAFKID 250
    GREIDPANLS LRGQFLHQGE IFWENSNCST KCRCLDFNNE IFCQEMLAPF 300
    ETVEPKIKFF QCVPVETACV VFGDPHYHTF DGFLFHFQGS CSYLLARQCW 350
    PGSQLPYFNV EAKNERGGSS VSWAEDIFVE VYRHKIVLPK GGFGKAKVDD 400
    LVVSLGAIKV YQSGLSTALE TDFGLLVTYD GQHYASVSVP GTYINGTCGL 450
    CGNYNKDPED DALRSDGRLA SSVPELGESW RVPHPERKCS PGCVENCSVC 500
    DASRILYSPI CGFSQECGAW SVLVATAFVH SCVYDLCSAR RTHRLCQAIQ 550
    VTLRCCQGLG IRWENGVPDG MRGGLAVPGH SHYSGCASGC PATCSDLTAP 600
    LRCTAPCPEG CECDDGHVLS ARPLHSLCRS GCVVDGRSRC REVFWATADC 650
    TAECQCEDGG EAKCFNTSCP EGEVCTIEDG YRGCYPKREG LCSVGQNQVL 700
    RTFDGVTFPY PLEHSYTLLK TCMEKPDFIE VDISQKKPDT LPMAGRVVRI 750
    QVVGQEVKVG GASLSEVKVN GYDVDLPYFH PSGHLEIYRT DNGTVTESEG 800
    LLSIGYYDSG LLEIRLSTAY FNCTGGLCGF FNGNDSDEFC TPKAKCTDNL 850
    ELFLESWTTF DEICNGECGD LLKACNNDSE LLKTYRSRSN CAIINDPTNS 900
    SFLECHNVSI VSAYYRTCLF RLCQSGGNQS ELCSAVARYA SACKNSEVDV 950
    GQWRSHSFCP LACPENSHFE ECMSCVETCE TLATGCCMDT CTEGCQCDEG 1000
    FALRSPCVPR GECGCNFEGH ELATNQTFWM DISCHLLCYC NGSDNSVYCE 1050
    NVLQDDEYYC HVRTDASCIV SGYGHYLTFD GFSFDFQSSC ALVLCTTIHG 1100
    ACERSDTFPT FTVTVTAKNE DRDTSLACVV KQVEVEVFNY YIVIHRAYKY 1150
    TVMINNERLY LPLKLGQGKV NIFAFGFHIV VETDFGLKVV YDWKTFLSVT 1200
    IPRSFQNLTY GLCGRYNGNP DDDLVAAGGT PRFGVTDFVQ SWAKRDTFCR 1250
    VGSGDRCPAC GKVEGFWKPQ QLCSLIPSQS GVFAKCHSKI NPSYFYKNCL 1300
    FDTVVDGGAM ARRVADWLQN YASTCQTQGI AIIGWRNFTS CSVSCPPNSH 1350
    YESCVSLCQP RCAAIRLKSD CGHYCVEGCQ CDPGYVLNGK SCILPQNCGC 1400
    YSDGKYYEPK QLFWNGDCTR RCARFRRNLI QCDPRHCKSD EECASRNGVR 1450
    GCFSTRSSFC LAAGGGVFRT FDGAFLRFPA NCAFVLSTIC QRLADFSFQL 1500
    IINFDKWSSP NLTIISVYIY INEEQILISD RSTVKVNGSL VSIPFVTGLS 1550
    TKIYSQEGFL VIDSGPDIHI RYNGFNVIKI TIGDRLQNKV CGLCGNFNGD 1600
    PADDYATLRG KPVVSSVVLA QSWKTNGMQK SCNELQYSQY AASCDNVQIQ 1650
    KLQSDSYCLK LTDMKGFFQP CYGLLDPLPF YESCFLDGCY NRKQVQLCGS 1700
    LAAYGEACRT FGILGTEWIE KENCSGVVED PCAGADCPNR TCELDDGGEL 1750
    CGCIEPPPYG NTTHDIIDAE VTCKAAQMEV SISKCKLFQL GFEREGVRVN 1800
    DRHCPGIEGE DFISFQINNT KGNCGNLVQS NSTHIVYKNT VWIESANNTG 1850
    NIITRDRTIN VEVFCAYELD IKISLDSVVR PMLSVINLTV PTQEGSFTTK 1900
    MALYKNSSYK HPYRQGEVVL TTRDVLYVGV FVVGADSNHL ILMLNKCYAT 1950
    PSRDSNDKLR YFIIEGGCQN LKDNTIGIEE NGVSLTCRFH VTVFKFIGDY 2000
    DEVHLHCAVS LCDSEKYSCK INCPQHRRSA SAFAQEAHEQ ILSVGPIRRK 2050
    RSDWCEDNGG CEQICTSQAD GPLCSCVTGT LQGDGKSCMA SSSSADIRAQ 2100
    ASLLVAAQLW LWAALHDPTS 2120
    Length:2,120
    Mass (Da):233,965
    Last modified:May 1, 1999 - v1
    Checksum:iE93F69EA18B51A4C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1114 – 11152Missing AA sequence (PubMed:9079715)Curated
    Sequence conflicti1123 – 11231Missing AA sequence (PubMed:9079715)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ012287 mRNA. Translation: CAA09979.1.
    PIRiT30243.
    RefSeqiNP_990204.1. NM_204873.1.
    UniGeneiGga.399.

    Genome annotation databases

    GeneIDi395686.
    KEGGigga:395686.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ012287 mRNA. Translation: CAA09979.1 .
    PIRi T30243.
    RefSeqi NP_990204.1. NM_204873.1.
    UniGenei Gga.399.

    3D structure databases

    ProteinModelPortali Q9YH85.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000010663.

    Proteomic databases

    PaxDbi Q9YH85.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 395686.
    KEGGi gga:395686.

    Organism-specific databases

    CTDi 7007.

    Phylogenomic databases

    eggNOGi NOG317991.
    HOVERGENi HBG079244.
    KOi K18273.
    PhylomeDBi Q9YH85.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16938.

    Miscellaneous databases

    NextBioi 20815758.
    PROi Q9YH85.

    Family and domain databases

    InterProi IPR000742. EG-like_dom.
    IPR003886. Nidogen_extracell_dom.
    IPR002919. TIL_dom.
    IPR025615. TILa_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR006552. VWC_out.
    IPR001846. VWF_type-D.
    IPR001507. ZP_dom.
    IPR017977. ZP_dom_CS.
    [Graphical view ]
    Pfami PF08742. C8. 3 hits.
    PF06119. NIDO. 1 hit.
    PF01826. TIL. 3 hits.
    PF12714. TILa. 2 hits.
    PF00094. VWD. 4 hits.
    PF00100. Zona_pellucida. 1 hit.
    [Graphical view ]
    SMARTi SM00832. C8. 3 hits.
    SM00181. EGF. 1 hit.
    SM00539. NIDO. 1 hit.
    SM00215. VWC_out. 1 hit.
    SM00216. VWD. 4 hits.
    SM00241. ZP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57567. SSF57567. 3 hits.
    PROSITEi PS51220. NIDO. 1 hit.
    PS51233. VWFD. 4 hits.
    PS00682. ZP_1. 1 hit.
    PS51034. ZP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chick alpha-tectorin: molecular cloning and expression during embryogenesis."
      Coutinho P., Goodyear R., Legan P.K., Richardson G.P.
      Hear. Res. 130:62-74(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Cochlear duct.
    2. "The mouse tectorins. Modular matrix proteins of the inner ear homologous to components of the sperm-egg adhesion system."
      Legan P.K., Rau A., Keene J.N., Richardson G.P.
      J. Biol. Chem. 272:8791-8801(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-33; 325-334 AND 1105-1124.
    3. "The protein composition of the avian tectorial membrane."
      Killick R., Malenczak C., Richardson G.P.
      Hear. Res. 64:21-38(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiTECTA_CHICK
    AccessioniPrimary (citable) accession number: Q9YH85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3