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Protein

Protein quaking

Gene

QKI

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of some pre-mRNA. Acts as a translational repressor (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, mRNA processing, mRNA splicing, mRNA transport, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein quaking
Gene namesi
Name:QKI
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Protein quakingPRO_0000239377Add
BLAST

Proteomic databases

PaxDbiQ9YH18.

Interactioni

Subunit structurei

Homodimer. Does not require RNA to homodimerize (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018821.

Structurei

3D structure databases

ProteinModelPortaliQ9YH18.
SMRiQ9YH18. Positions 81-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 15367KHAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi275 – 2784SH3-binding

Sequence similaritiesi

Contains 1 KH domain.Curated

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG1588. Eukaryota.
COG5176. LUCA.
HOGENOMiHOG000019831.
HOVERGENiHBG093883.
InParanoidiQ9YH18.
KOiK14945.
PhylomeDBiQ9YH18.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032367. Quaking_NLS.
IPR032377. STAR_dimer.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16551. Quaking_NLS. 1 hit.
PF16544. STAR_dimer. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9YH18-1) [UniParc]FASTAAdd to basket

Also known as: QkI-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGEMEAKEK PKPSPDYLMQ LMNDKKLMSS LPNFCGIFNH LERLLDEEIS
60 70 80 90 100
RVRKDMYNDT LNGSTEKRSA ELPDAVGPIV QLQEKLYVPV KEYPDFNFVG
110 120 130 140 150
RILGPRGLTA KQLEAETGCK IMVRGKGSMR DKKKEEQNRG KPNWEHLNED
160 170 180 190 200
LHVLITVEDA QNRAEIKLKR AVEEVKKLLI PAAEGEDSLK KMQLMELAIL
210 220 230 240 250
NGTYRDANIK SPALAFSLAA TAQAPRIITG PAPVLPPAAL RTPTPAGPTI
260 270 280 290 300
MPLIRQIQTA VMPNGTPHPT AAIVPPGPEA GLIYTPYEYP YTLAPATSIL
310 320 330 340
EYPIEPSGVL GAVATKVRRH DMRVHPYQRI VTADRAATGN
Note: Major isoform.
Length:340
Mass (Da):37,570
Last modified:June 13, 2006 - v2
Checksum:iB3129274D1BB0D45
GO
Isoform 2 (identifier: Q9YH18-2) [UniParc]FASTAAdd to basket

Also known as: QkI-2

The sequence of this isoform differs from the canonical sequence as follows:
     213-220: Missing.

Show »
Length:332
Mass (Da):36,825
Checksum:i4EDEAE72DCCDE199
GO
Isoform 3 (identifier: Q9YH18-3) [UniParc]FASTAAdd to basket

Also known as: QkI-3

The sequence of this isoform differs from the canonical sequence as follows:
     134-134: K → KVSFKSRDNHDPAVLEVE
     213-220: Missing.

Show »
Length:349
Mass (Da):38,749
Checksum:i71D640901832F84B
GO
Isoform 4 (identifier: Q9YH18-4) [UniParc]FASTAAdd to basket

Also known as: QkI-4

The sequence of this isoform differs from the canonical sequence as follows:
     184-186: EGE → WET
     187-340: Missing.

Show »
Length:186
Mass (Da):21,334
Checksum:iA36DEA18470DABC1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441W → R in AAD00624 (PubMed:9547512).Curated
Sequence conflicti206 – 2061D → G in AAD00623 (PubMed:9547512).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei134 – 1341K → KVSFKSRDNHDPAVLEVE in isoform 3. 1 PublicationVSP_019200
Alternative sequencei184 – 1863EGE → WET in isoform 4. 1 PublicationVSP_019201
Alternative sequencei187 – 340154Missing in isoform 4. 1 PublicationVSP_019202Add
BLAST
Alternative sequencei213 – 2208Missing in isoform 2 and isoform 3. 1 PublicationVSP_019203

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86764 mRNA. Translation: AAD00621.1.
U86765 Genomic DNA. Translation: AAD00622.1.
U86766 mRNA. Translation: AAD00623.1.
U86767 mRNA. Translation: AAD00624.1.
RefSeqiNP_989641.2. NM_204310.2. [Q9YH18-1]
XP_015139487.1. XM_015284001.1. [Q9YH18-3]
XP_015139489.1. XM_015284003.1. [Q9YH18-2]
UniGeneiGga.3168.

Genome annotation databases

GeneIDi374204.
KEGGigga:374204.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86764 mRNA. Translation: AAD00621.1.
U86765 Genomic DNA. Translation: AAD00622.1.
U86766 mRNA. Translation: AAD00623.1.
U86767 mRNA. Translation: AAD00624.1.
RefSeqiNP_989641.2. NM_204310.2. [Q9YH18-1]
XP_015139487.1. XM_015284001.1. [Q9YH18-3]
XP_015139489.1. XM_015284003.1. [Q9YH18-2]
UniGeneiGga.3168.

3D structure databases

ProteinModelPortaliQ9YH18.
SMRiQ9YH18. Positions 81-214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018821.

Proteomic databases

PaxDbiQ9YH18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi374204.
KEGGigga:374204.

Organism-specific databases

CTDi9444.

Phylogenomic databases

eggNOGiKOG1588. Eukaryota.
COG5176. LUCA.
HOGENOMiHOG000019831.
HOVERGENiHBG093883.
InParanoidiQ9YH18.
KOiK14945.
PhylomeDBiQ9YH18.

Miscellaneous databases

PROiQ9YH18.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032367. Quaking_NLS.
IPR032377. STAR_dimer.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16551. Quaking_NLS. 1 hit.
PF16544. STAR_dimer. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Four isoforms of the signal-transduction and RNA-binding protein QKI expressed during chicken spermatogenesis."
    Mezquita J., Pau M., Mezquita C.
    Mol. Reprod. Dev. 50:70-78(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: Hubbard White Mountain.
    Tissue: Testis.

Entry informationi

Entry nameiQKI_CHICK
AccessioniPrimary (citable) accession number: Q9YH18
Secondary accession number(s): Q9PST2, Q9YH19, Q9YH20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: June 8, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.