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Protein

Single-strand selective monofunctional uracil DNA glycosylase

Gene

smug1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. Does not exhibit any enzymatic activity towards G/T mismatches.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951Substrate; via amide nitrogen
Binding sitei109 – 1091Substrate; via amide nitrogen
Binding sitei174 – 1741Substrate
Binding sitei250 – 2501Substrate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Single-strand selective monofunctional uracil DNA glycosylase (EC:3.2.2.-)
Gene namesi
Name:smug1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-953126. smug1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Single-strand selective monofunctional uracil DNA glycosylasePRO_0000071995Add
BLAST

Structurei

Secondary structure

1
281
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi39 – 5416Combined sources
Beta strandi64 – 663Combined sources
Helixi68 – 714Combined sources
Helixi73 – 8311Combined sources
Beta strandi89 – 968Combined sources
Turni99 – 1013Combined sources
Helixi102 – 1054Combined sources
Helixi112 – 1176Combined sources
Helixi139 – 1413Combined sources
Helixi147 – 16014Combined sources
Helixi163 – 1675Combined sources
Beta strandi170 – 1756Combined sources
Beta strandi179 – 1813Combined sources
Helixi190 – 1923Combined sources
Helixi195 – 21622Combined sources
Beta strandi219 – 2257Combined sources
Helixi226 – 23813Combined sources
Beta strandi244 – 2485Combined sources
Helixi256 – 2583Combined sources
Turni259 – 2613Combined sources
Helixi262 – 27312Combined sources
Helixi276 – 2794Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OE4X-ray2.00A/B35-281[»]
1OE5X-ray2.30A/B35-281[»]
1OE6X-ray2.65A/B35-281[»]
ProteinModelPortaliQ9YGN6.
SMRiQ9YGN6. Positions 36-269.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9YGN6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni182 – 19817DNA bindingAdd
BLAST

Phylogenomic databases

HOVERGENiHBG084399.
KOiK10800.

Family and domain databases

Gene3Di3.40.470.10. 1 hit.
InterProiIPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9YGN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEACVPAE FSKDEKNGSI LSAFCSDIPD ITSSTESPAD SFLKVELELN
60 70 80 90 100
LKLSNLVFQD PVQYVYNPLV YAWAPHENYV QTYCKSKKEV LFLGMNPGPF
110 120 130 140 150
GMAQTGVPFG EVNHVRDWLQ IEGPVSKPEV EHPKRRIRGF ECPQSEVSGA
160 170 180 190 200
RFWSLFKSLC GQPETFFKHC FVHNHCPLIF MNHSGKNLTP TDLPKAQRDT
210 220 230 240 250
LLEICDEALC QAVRVLGVKL VIGVGRFSEQ RARKALMAEG IDVTVKGIMH
260 270 280
PSPRNPQANK GWEGIVRGQL LELGVLSLLT G
Length:281
Mass (Da):31,228
Last modified:May 1, 1999 - v1
Checksum:iB92292E5B77CD007
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125181 mRNA. Translation: AAD17300.1.
BC090216 mRNA. Translation: AAH90216.1.
RefSeqiNP_001083825.1. NM_001090356.1.
UniGeneiXl.44615.

Genome annotation databases

GeneIDi399139.
KEGGixla:399139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125181 mRNA. Translation: AAD17300.1.
BC090216 mRNA. Translation: AAH90216.1.
RefSeqiNP_001083825.1. NM_001090356.1.
UniGeneiXl.44615.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OE4X-ray2.00A/B35-281[»]
1OE5X-ray2.30A/B35-281[»]
1OE6X-ray2.65A/B35-281[»]
ProteinModelPortaliQ9YGN6.
SMRiQ9YGN6. Positions 36-269.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399139.
KEGGixla:399139.

Organism-specific databases

CTDi23583.
XenbaseiXB-GENE-953126. smug1.

Phylogenomic databases

HOVERGENiHBG084399.
KOiK10800.

Miscellaneous databases

EvolutionaryTraceiQ9YGN6.

Family and domain databases

Gene3Di3.40.470.10. 1 hit.
InterProiIPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSMUG1_XENLA
AccessioniPrimary (citable) accession number: Q9YGN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: May 1, 1999
Last modified: October 14, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.