ID   PIT1_CHICK              Reviewed;         363 AA.
AC   Q9YGL7; Q9YGL8;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   27-MAR-2024, entry version 134.
DE   RecName: Full=Pituitary-specific positive transcription factor 1;
DE            Short=PIT-1;
DE   AltName: Full=Growth hormone factor 1;
DE            Short=GHF-1;
GN   Name=POU1F1; Synonyms=PIT1;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PIT-1* AND PIT-1BETA*).
RC   STRAIN=Ross; TISSUE=Pituitary;
RA   van As P., Buys N., Volckaert G., Decuypere E.;
RT   "Complementary DNA cloning of pituitary specific transcription factor (Pit-
RT   1) from chicken.";
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor that activates growth hormone and
CC       prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-
CC       3'. {ECO:0000250|UniProtKB:P28069}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P28069}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=PIT-1beta*;
CC         IsoId=Q9YGL7-1; Sequence=Displayed;
CC       Name=PIT-1;
CC         IsoId=Q9YGL7-3; Sequence=Not described;
CC       Name=PIT-1*;
CC         IsoId=Q9YGL7-2; Sequence=VSP_002317;
CC   -!- TISSUE SPECIFICITY: Pituitary gland.
CC   -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC       number of yeast and animal transcription factors.
CC       {ECO:0000250|UniProtKB:P28069}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-1
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ236856; CAB37357.1; -; mRNA.
DR   EMBL; AJ236855; CAB37356.1; -; mRNA.
DR   AlphaFoldDB; Q9YGL7; -.
DR   SMR; Q9YGL7; -.
DR   STRING; 9031.ENSGALP00000045301; -.
DR   PaxDb; 9031-ENSGALP00000036048; -.
DR   VEuPathDB; HostDB:geneid_374215; -.
DR   eggNOG; KOG3802; Eukaryota.
DR   InParanoid; Q9YGL7; -.
DR   PhylomeDB; Q9YGL7; -.
DR   Proteomes; UP000000539; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   PANTHER; PTHR11636:SF84; PITUITARY-SPECIFIC POSITIVE TRANSCRIPTION FACTOR 1; 1.
DR   PANTHER; PTHR11636; POU DOMAIN; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..363
FT                   /note="Pituitary-specific positive transcription factor 1"
FT                   /id="PRO_0000100703"
FT   DOMAIN          195..269
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        285..344
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   MOTIF           5..13
FT                   /note="9aaTAD"
FT                   /evidence="ECO:0000250|UniProtKB:P28069"
FT   VAR_SEQ         48..75
FT                   /note="Missing (in isoform PIT-1*)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_002317"
SQ   SEQUENCE   363 AA;  40449 MW;  424E43A40D28DDDB CRC64;
     MTCQAFASSD NFVPLNSDSS PSLPLIMHHS AGECLPVSNH ATNVVSTVPS VLSLIQTPKC
     SHFHFAMMTS GNVSAGLHYS VPSCHYGNQA STYGVMAGIK PATPEMLSAS LSQSRILQTC
     SMPHPNVVNG VSTLQSSLTP CLYKFPEHAL SASSCALGHS FTPMHQTLLS DDPTASDFKQ
     EFRRKSKSVE EPVDMDSPEI RELEKFANEF KLRRIKLGYT QTNVGEALAA VHGSEFSQTT
     ICRFENLQLS FKNACKLKSI LSKWLEEAEQ VGALYNEKVG VNERKRKRRT TISISAKEAL
     ERHFGEQSKP SSQEIMRMAE GLNLEKEVVR VWFCNRRQRE KRVKTSLHQN AFSSIIKEHH
     ECR
//