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Protein

Deoxyribonuclease-1

Gene

DNASE1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Divalent metal cations. Prefers Ca2+ or Mg2+.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98By similarity1
Active sitei154By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Endonuclease, Hydrolase, Nuclease
Biological processApoptosis
LigandCalcium

Enzyme and pathway databases

BRENDAi3.1.21.1. 1306.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-1 (EC:3.1.21.1)
Alternative name(s):
Deoxyribonuclease I
Short name:
DNase I
Gene namesi
Name:DNASE1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Secreted By similarity
  • Nucleus envelope By similarity

  • Note: Secretory protein, stored in zymogen granules and found in the nuclear envelope.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000728221 – 282Deoxyribonuclease-1Add BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi121 ↔ 124By similarity
Disulfide bondi193 ↔ 229Essential for enzymatic activityBy similarity

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9YGI5.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000029321.

Structurei

3D structure databases

ProteinModelPortaliQ9YGI5.
SMRiQ9YGI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFZB. Eukaryota.
ENOG410Z4MV. LUCA.
HOVERGENiHBG051368.
InParanoidiQ9YGI5.
KOiK11994.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiView protein in InterPro
IPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR033125. DNASE_I_2.
IPR005135. Endo/exonuclease/phosphatase.
PfamiView protein in Pfam
PF03372. Exo_endo_phos. 1 hit.
PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
PRINTSiPR00130. DNASEI.
SMARTiView protein in SMART
SM00476. DNaseIc. 1 hit.
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiView protein in PROSITE
PS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YGI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLVLELLA AALLLRVAAT LRISAFNIRT FGDSKMSNQT VAGFIVSILV
60 70 80 90 100
QYDITLVQEV RDADLSSVKK LVSQLNSASS YPYSFLSSIP LGRNSYKEQY
110 120 130 140 150
VFIYRSDIVS VLESYYYDDG CESCGTDIFS REPFIVKFSS PTTQLDEFVI
160 170 180 190 200
VPLHAEPSSA PAEINALTDV YTDVINKWET NNIFFMGDFN ADCSYVTAEQ
210 220 230 240 250
WPSIRLRSLS SCEWLIPDSA DTTVTSTDCA YDRIVACGSA LRQAVEYGSA
260 270 280
TVNNFQETLR IQNKDALAIS DHFPVEVTLK AR
Length:282
Mass (Da):31,400
Last modified:May 1, 1999 - v1
Checksum:i2226267860842EA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131751 mRNA. Translation: CAA10503.1.
RefSeqiNP_996840.1. NM_206957.1.
UniGeneiGga.287.
Gga.41568.

Genome annotation databases

GeneIDi395725.
KEGGigga:395725.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDNAS1_CHICK
AccessioniPrimary (citable) accession number: Q9YGI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1999
Last modified: July 5, 2017
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families