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Protein

Interleukin-1 beta

Gene

il1b

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.By similarity

GO - Biological processi

  • cortisol secretion Source: AgBase
  • fever generation Source: UniProtKB-KW
  • immune response Source: InterPro
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of gene expression Source: AgBase
  • positive regulation of leukocyte chemotaxis Source: AgBase
  • positive regulation of phagocytosis Source: AgBase
  • positive regulation of protein phosphorylation Source: AgBase
  • regulation of calcium ion transport Source: AgBase
  • T cell proliferation Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Mitogen, Pyrogen

Keywords - Biological processi

Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-1 beta
Gene namesi
Name:il1b
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Lysosome By similarity
  • Secretedexosome By similarity
  • Cytoplasmic vesicleautophagosome By similarity
  • Secreted By similarity

  • Note: The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood. On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca2+-dependent exocytosis with release of mature IL1B. 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes. However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes. 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis. 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome. These mechanisms may not be not mutually exclusive.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 260Interleukin-1 betaPRO_0000015324
Propeptidei1 – ?PRO_0000015323

Expressioni

Tissue specificityi

All three isoforms are expressed in gill, kidney, liver and spleen. Isoform 1 is also expressed in blood. None of the isoforms are expressed in brain.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OOXmodel-A95-260[»]
ProteinModelPortaliQ9YGD3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-1 family.Curated

Phylogenomic databases

HOVERGENiHBG106914.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR003294. IL-1_alpha/beta.
IPR003296. IL-1_beta.
IPR000975. IL-1_fam.
[Graphical view]
PANTHERiPTHR10078:SF20. PTHR10078:SF20. 1 hit.
PfamiPF00340. IL1. 1 hit.
[Graphical view]
PRINTSiPR00264. INTERLEUKIN1.
PR01357. INTRLEUKN1AB.
SUPFAMiSSF50353. SSF50353. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YGD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFESNYSLI KNTSESAAWS SKLPQGLDLE VSHHPITMRH IANLIIAMER
60 70 80 90 100
LKGGEGVTMG TEFKDKDLLN FLLESAVEEH IVLELESAPP ASRRAAGFSS
110 120 130 140 150
TSQYECSVTD SENKCWVLMN EAMELHAMML QGGSSYHKVH LNLSSYVTPV
160 170 180 190 200
PIETEARPVA LGIKGSNLYL SCSKSGGRPT LHLEEVADKD QLKSISQQSD
210 220 230 240 250
MVRFLFYRRN TGVDISTLES ASFRNWFIST DMQQDYTKPV DMCQKAAPNR
260
LTTFTIQRHN
Length:260
Mass (Da):29,127
Last modified:May 1, 1999 - v1
Checksum:i0DC77991EA53E97F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ004821 Genomic DNA. Translation: CAA06157.1.
AJ223954 mRNA. Translation: CAA11684.1.
AJ278242 Genomic DNA. Translation: CAB93352.1.
RefSeqiNP_001117819.1. NM_001124347.2.
UniGeneiOmy.11696.

Genome annotation databases

GeneIDi100136024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ004821 Genomic DNA. Translation: CAA06157.1.
AJ223954 mRNA. Translation: CAA11684.1.
AJ278242 Genomic DNA. Translation: CAB93352.1.
RefSeqiNP_001117819.1. NM_001124347.2.
UniGeneiOmy.11696.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OOXmodel-A95-260[»]
ProteinModelPortaliQ9YGD3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100136024.

Phylogenomic databases

HOVERGENiHBG106914.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR003294. IL-1_alpha/beta.
IPR003296. IL-1_beta.
IPR000975. IL-1_fam.
[Graphical view]
PANTHERiPTHR10078:SF20. PTHR10078:SF20. 1 hit.
PfamiPF00340. IL1. 1 hit.
[Graphical view]
PRINTSiPR00264. INTERLEUKIN1.
PR01357. INTRLEUKN1AB.
SUPFAMiSSF50353. SSF50353. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIL1B_ONCMY
AccessioniPrimary (citable) accession number: Q9YGD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: May 1, 1999
Last modified: December 9, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

IL1B production occurs in 2 steps, each being controlled by different stimuli. First, inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1B (priming). Then additional signals are required for inflammasome assembly, leading to CASP1 activation, pro-IL1B processing and eventually secretion of the active cytokine. IL1B processing and secretion are temporarily associated.By similarity

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.