Q9YGB2 (TRPG_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component II
EC=4.1.3.27

Alternative name(s):
Glutamine amido-transferase

Gene names

Name:	trpG
Ordered Locus Names:	TK0255

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Contains 2 chains with different activities: component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Anthranilate synthase component II		PRO_0000056891
Regions
	Domain	1 – 192	192	Glutamine amidotransferase type-1
Sites
	Active site	78	1	By similarity
	Active site	165	1	By similarity
	Active site	167	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YGB2 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: ACF9E3F01FC6E1A6	FASTA	192	21,156
	10 20 30 40 50 60 MIVLVNNRDS FVWNLAEYAS LFDRVKVVPN TITVGELRRL DPDGVIISPG PGHPLERREV 70 80 90 100 110 120 GNSPEIVLEA GVPILGVCLG HQIIATAFGG KVGRVKPRHG KASPVKHDGK GVLRGIKNPL 130 140 150 160 170 180 TAGRYHSLAV LEVPREFDVS AVSLDDNVVM GIRHRKLPIE GLQFHPESVL TEWERKEGLR 190 IIKNFVEMSR NG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The tryptophan biosynthesis gene cluster trpCDEGFBA from Pyrococcus kodakaraensis KOD1 is regulated at the transcriptional level and expressed as a single mRNA." Tang X., Ezaki S., Fujiwara S., Takagi M., Atomi H., Imanaka T. Mol. Gen. Genet. 262:815-821(1999) [PubMed: 10628865] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]	"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB030011 Genomic DNA. Translation: BAA82548.1. AP006878 Genomic DNA. Translation: BAD84444.1.
PIR	T43925.
RefSeq	YP_182668.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q9YGB2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000008229; EBPYRP00000008010; EBPYRG00000008228.
GeneID	3235374.
GenomeReviews	Gene locus TK0255 in contig AP006878_GR.
KEGG	tko:TK0255.
NMPDR	fig|69014.3.peg.2.
Phylogenomic databases
eggNOG	arNOG07708.
GeneTree	EBGT00050000022457.
HOGENOM	HBG292341.
OMA	REHPIEA.
PhylomeDB	Q9YGB2.
ProtClustDB	PRK05670.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0255-MONOMER.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR006221. TrpG_papA. [Graphical view]
KO	K01658.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00566. TrpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPG_PYRKO

Accession

Primary (citable) accession number: Q9YGB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program