Reviewed, UniProtKB/Swiss-Prot Q9YG88 (ILVD_AERPE)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	APE_0013.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103536
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9YG88-1 [UniParc]. Last modified June 26, 2007. Version 3. Checksum: 8F24DDF5BBF49539	FASTA	553	58,605
	10 20 30 40 50 60 MKRPRSRLWY DGLENTPHRV YLRAIGFTSD DFQKPLIAVV AAWSEAGPCN FNVLPGSLRV 70 80 90 100 110 120 KEGVRSAGGV PLAVPTIVVN DGINMGTPGM RYSLISRELI ADTIEAQVAS HGFDGWVGIG 130 140 150 160 170 180 GCDKTQPGIM MAMARLDLPS IYIYGGTAEH GVLDGETVTV QSAFEAVGAY LKGLIDEERL 190 200 210 220 230 240 YEIEKAAMPT PGTCQGLFTA NTMAILAEAL GLSPLGSASP PATSSERARE LARAGALAVG 250 260 270 280 290 300 LVETGLTPRR ILTYEAFYNA IVTLMAISGS TNAVLHLLAI AREAGVKLAL DDFDEASRKV 310 320 330 340 350 360 PVIAALAPAG KYTMVDLHNV GGAPVILRKL LDRGLLYGEA VTVEGVEIGK LLSKWEPRTD 370 380 390 400 410 420 YNILYDFDKP YKPHAGLRIL RGSLAPRGAV MKIGASGILK FKGAAKVFDS EEEAFKAIER 430 440 450 460 470 480 GYVEEGDVVV VRYVGPKGAP GMPEMLKITA AIVGAGLGEK VALITDGRFS GATRGVMVGH 490 500 510 520 530 540 AAPEAAVGGP IALVENGDEI VIDGEKGTLD VLLGGDELAR RRDKWSPPPL PKQGLLRKYA 550 KLVTQADEGA VTH

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000002 Genomic DNA. Translation: BAA78922.2.
PIR	H72752.
RefSeq	NP_146903.2.
3D structure databases
SMR	Q9YG88. Positions 65-553.
ModBase	Search...
Genome annotation databases
GeneID	1445585.
GenomeReviews	Gene locus APE_0013.1 in contig BA000002_GR.
KEGG	ape:APE_0013.1.
NMPDR	fig|272557.1.peg.10.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
Enzyme and pathway databases
BioCyc	APER272557:APE0013-MONOMER.
BRENDA	4.2.1.9. 256344.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_AERPE

Accession

Primary (citable) accession number: Q9YG88

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 26, 2007
Last modified:	February 9, 2010
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)