Reviewed, UniProtKB/Swiss-Prot Q9YFY3 (SYT2_AERPE)

Names and origin

Protein names

Recommended name:
    Probable threonyl-tRNA synthetase 2
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase 2
      Short name=ThrRS 2

Gene names

Name:	thrS2
Ordered Locus Names:	APE_0117.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Subcellular location	Cytoplasm Potential.
Miscellaneous	There are two ThrRS in this archaea. The first one (APE_0809.1) is most similar to bacterial ThrRS but it lack the N-terminal domain. The second one (APE_0117.1) is most similar to archaeal ThrRS but lacks the central domain. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Probable threonyl-tRNA synthetase 2 HAMAP MF_00184		PRO_0000101118

Sequences

	Sequence		Length	Mass (Da)
	Q9YFY3-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 9B311637CE9FBC73	FASTA	421	45,003
	10 20 30 40 50 60 MRLLYLHADR FEYKTVKPAL KNPPDPPGEA SFGEALVVFT TVEDGDGPQT VMYAASDIAS 70 80 90 100 110 120 HSSRLKVTTV ILYPYAHLSS RLAKPMAAHK RLIELEGALR TKFPGHVHRA PFGWYKSFSI 130 140 150 160 170 180 ACKGHPLAEL SRSFTEAGAL QPWPAVEDYK TGLSGEVLAR AGLLGGGSLS PASWALEVHR 190 200 210 220 230 240 RLAEEVVGPA ESVGFGESLS EAYQACISSS VTTLLMGPYP PSIVFGPLED DPVEAVSRVL 250 260 270 280 290 300 GLISPQLEGV KPLLSGGEGA LKASSPDGAE LPVAFLKEGR VCLGPTLSFF KLAVSMLVEK 310 320 330 340 350 360 ARKEGLTPYL NPTLTPVQSA VIPVDSESEG YAQRIAEDLA ASGVRVSIVR GSGLGRRVRE 370 380 390 400 410 420 AGRSWASLVI VVGKREEETG TVVVRRRWEP GKQEVLTLDE LSSEAKKLAS GSRGSLYSTT L

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA79028.2.
PIR	B72766.
RefSeq	NP_146977.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1445659.
GenomeReviews	Gene locus APE_0117.1 in contig BA000002_GR.
KEGG	ape:APE_0117.1.
NMPDR	fig|272557.1.peg.84.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BRENDA	6.1.1.3. 256344.
Family and domain databases
HAMAP	MF_00184. Divergent sequence. [Tree]
InterPro	IPR004154. Anticodon_bd. IPR015011. Threonyl-tRNA_syntase_arc. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF08915. tRNA-Thr_ED. 1 hit. [Graphical view]
PROSITE	PS50862. AA_TRNA_LIGASE_II. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT2_AERPE

Accession

Primary (citable) accession number: Q9YFY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 26, 2007
Last modified:	July 28, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)