Reviewed, UniProtKB/Swiss-Prot Q9YFI6 (PYRD_AERPE)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	APE_0260.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00224
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00224
Subunit structure	Heterotetramer of 2 pyrK and 2 pyrD subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Dihydroorotate dehydrogenase HAMAP MF_00224		PRO_0000148406
Sites
	Active site	132	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YFI6-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: B07059307532510D	FASTA	306	32,121
	10 20 30 40 50 60 MYSQPELSIS IAGLRLQRPV GNASGILGWE PREARLVEEG GGGFFVAKSV TYQPRKGYPQ 70 80 90 100 110 120 PHLYPVAGGI VNAVGLANPG FREASKMLAQ TVEEASIPVI ASIAGGAPGE WVEMASTLEE 130 140 150 160 170 180 AGVSAVELNL SCPHFAGGGL ELGQDPAAVA SVVSAVASTL RIPVIAKLGY SDRLVDAASK 190 200 210 220 230 240 ALEAGARGLT LINSMRAMKI DVYAKKPVLG NRVGGLSGKP IHPIAVRAVY EVYGETRADI 250 260 270 280 290 300 FAAGGVESWE DAVEFYLAGA KAVQVGAAFI TIGPHVLRSI VEGVRRYLWV EGFRSLQDIV GYAHRA

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA79175.2.
PIR	E72784.
RefSeq	NP_147090.2.
3D structure databases
HSSP	HSSP built from PDB template 1EP3 based on UniProtKB P54322.
ModBase	Search...
Genome annotation databases
GeneID	1445772.
GenomeReviews	Gene locus APE_0260.1 in contig BA000002_GR.
KEGG	ape:APE_0260.1.
NMPDR	fig|272557.1.peg.197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YFI6.
Enzyme and pathway databases
BRENDA	1.3.3.1. 256344.
Family and domain databases
HAMAP	MF_00224. [Tree]
InterPro	IPR012135. Dihydroorotate_DH_1_2. IPR005720. Dihydroorotate_DH_1_core. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. False negative. PS00912. DHODEHASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_AERPE

Accession

Primary (citable) accession number: Q9YFI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 26, 2007
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)