Reviewed, UniProtKB/Swiss-Prot Q9YFI5 (PYRC_AERPE)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	APE_0261.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Dihydroorotase		PRO_0000147266
Regions
	Compositional bias	80 – 83	4	Poly-Ala
Sites
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 1 By similarity
	Metal binding	175	1	Zinc 2 By similarity
	Metal binding	224	1	Zinc 2 By similarity
	Metal binding	293	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YFI5-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: AC727B22314AE0F0	FASTA	425	46,174
	10 20 30 40 50 60 MAGWTVCVDF LYTGDKMLED ACAVISGERV ERVHTGGFRG QPDLDLRGRS SLALPGVIDM 70 80 90 100 110 120 HVHLRGLKLS YKEDEVSGTA AAASGCVTLV ADMPNTQPPL RTPEALREKL ASLEAGAKVD 130 140 150 160 170 180 YTVYAGVPRS YGEAVEMASM GIAGFKIYPE DLVNLDSIKN VLEAAEEAGI LVVLHPESHD 190 200 210 220 230 240 MFAGPDYGWL RRVARPCHAE AASVELLHDA ARECGCKPRV HITHASCPAT VVESKKRGFT 250 260 270 280 290 300 VDVTPHHLFL SEEEVAGPTH SWGKVNPPLR GVGERSLLTQ LTIEGLVDAI ASDHAPHSTW 310 320 330 340 350 360 EKWMHPAIAP PGFPWLEWWP GFTAARLMRA VGLERFYSMV SQTPSTILGV GDRCLWDGCP 370 380 390 400 410 420 ATISVLQLER TRVYHKGYTK ALYTPVLGME SFDCAATVIR GKIAYTAWEG VRGGATGVLA TRVVH

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA79176.2.
PIR	F72784.
RefSeq	NP_147091.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1445773.
GenomeReviews	Gene locus APE_0261.1 in contig BA000002_GR.
KEGG	ape:APE_0261.1.
NMPDR	fig|272557.1.peg.198.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YFI5.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

PYRC_AERPE

Accession

Primary (citable) accession number: Q9YFI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 26, 2007
Last modified:	July 22, 2008
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)