SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9YF67

- SYI_AERPE

UniProt

Q9YF67 - SYI_AERPE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, APE_0374
Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei606 – 6061ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAPER272557:GJD6-283-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:APE_0374
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
ProteomesiUP000002518: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10641064Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098574Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272557.APE_0374.

Structurei

3D structure databases

ProteinModelPortaliQ9YF67.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6412"HIGH" regionUniRule annotation
Add
BLAST
Motifi603 – 6075"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiERLMLHQ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9YF67-1 [UniParc]FASTAAdd to Basket

« Hide

MGSVPRVLEG ERYDQFALES FVKSYWEENS IYRKVKEKSS RAPRKFYFLD     50
GPPYASAKSI HIGTAWNKVV KDVVLRFYRM TGYNVWDKPG YDTHGLPIEV 100
KIEQSLGVRV KREIEEKVGV ENFIAACKRF VDENMEAMTR QFKEIGVFMD 150
WENPYVTYRD EYIESGWWLV KKAWEKGLLY KGYRVLHWCP RCETTLADYE 200
VSEYRELEDP SIYVKFPVEG REGEYLLVWT TTPWTLPANA FVMAHPDMEY 250
VKVRVGRDTL ILAKARLDHV MREAGVSEYE VLEVLKGSQL EGLRYRHPLE 300
DVVDAQRELS KWHKVVMAPE AVTPHEGSGL VHSAPGHGTI DYEIAQRIGA 350
PVVSLVDERG FMTEAAGKYS GLYFRGEANK AIVEDLRARG ALFHAATIVH 400
RYPVCWRCKT PLLLRATTQW FVAVTRLKNQ LMREAEKVEW QPGWAKTRFM 450
NMLRELRDWV ISRQRYWGIP LPVWRCSSCG YEHVVGSVEE LESMGGRRPE 500
NLHRPWVDRV ELKCPRCGGS MKRVPDVLDV WFDSGIAFYA SLGYPRDRET 550
WERLKPVDFI VEGHDQIRGW FFSLLRSGVI GFGETPYRRV LVHGFALDEQ 600
GREMHKSLGN YVDFEELIAK VPRDVVRFWV LQNTVWEDLR FSWKGLDLMR 650
RDFNVIWNVY AFASIYMGLD RFDPRRTSLS SVEDSLEVED RWILSRLASL 700
KRRYLTAMED LRIHDAARAL RSFIIEDVSH WYLRIIRRRV WEEVDTPSKR 750
AAYATLYKVL WEWLLMAAPL IPYTAEYLYI KVFREAEERP EESIHMLDMP 800
EPEDHLIDST LEEDMEKARM VLEAILSARN EAGLKLRRPV RRVIIAASNP 850
GVEGSLRRLE GLIRLMANAK EIEFAPASAL EATRIYRVEP DYGRLGPKYK 900
RDMPKVLRLI EEKGGEIGRV LAEKGVYEGE YEGLPVKLTL EDVSLKAEYP 950
EWLKVRDTPL GLVAVDTRLT KEEVLEGLAR DIVRRIQAMR KEAGYSVEDY 1000
VEVWIQGGED VVEAVNALKE YIKRETRALK LEVGEPPSGV EVLREWELDG 1050
EKVVIALKRA AASG 1064
Length:1,064
Mass (Da):123,482
Last modified:November 1, 1999 - v1
Checksum:iDCB292AD67031978
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000002 Genomic DNA. Translation: BAA79329.1.
PIRiE72729.
RefSeqiNP_147189.1. NC_000854.2.
WP_010865700.1. NC_000854.2.

Genome annotation databases

EnsemblBacteriaiBAA79329; BAA79329; APE_0374.
GeneIDi1444584.
KEGGiape:APE_0374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000002 Genomic DNA. Translation: BAA79329.1 .
PIRi E72729.
RefSeqi NP_147189.1. NC_000854.2.
WP_010865700.1. NC_000854.2.

3D structure databases

ProteinModelPortali Q9YF67.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272557.APE_0374.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA79329 ; BAA79329 ; APE_0374 .
GeneIDi 1444584.
KEGGi ape:APE_0374.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ERLMLHQ.

Enzyme and pathway databases

BioCyci APER272557:GJD6-283-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiSYI_AERPE
AccessioniPrimary (citable) accession number: Q9YF67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi