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Q9YF67

- SYI_AERPE

UniProt

Q9YF67 - SYI_AERPE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei606 – 6061ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAPER272557:GJD6-283-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:APE_0374
    OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
    Taxonomic identifieri272557 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
    ProteomesiUP000002518: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10641064Isoleucine--tRNA ligasePRO_0000098574Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272557.APE_0374.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9YF67.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi53 – 6412"HIGH" regionAdd
    BLAST
    Motifi603 – 6075"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiERLMLHQ.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9YF67-1 [UniParc]FASTAAdd to Basket

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    MGSVPRVLEG ERYDQFALES FVKSYWEENS IYRKVKEKSS RAPRKFYFLD     50
    GPPYASAKSI HIGTAWNKVV KDVVLRFYRM TGYNVWDKPG YDTHGLPIEV 100
    KIEQSLGVRV KREIEEKVGV ENFIAACKRF VDENMEAMTR QFKEIGVFMD 150
    WENPYVTYRD EYIESGWWLV KKAWEKGLLY KGYRVLHWCP RCETTLADYE 200
    VSEYRELEDP SIYVKFPVEG REGEYLLVWT TTPWTLPANA FVMAHPDMEY 250
    VKVRVGRDTL ILAKARLDHV MREAGVSEYE VLEVLKGSQL EGLRYRHPLE 300
    DVVDAQRELS KWHKVVMAPE AVTPHEGSGL VHSAPGHGTI DYEIAQRIGA 350
    PVVSLVDERG FMTEAAGKYS GLYFRGEANK AIVEDLRARG ALFHAATIVH 400
    RYPVCWRCKT PLLLRATTQW FVAVTRLKNQ LMREAEKVEW QPGWAKTRFM 450
    NMLRELRDWV ISRQRYWGIP LPVWRCSSCG YEHVVGSVEE LESMGGRRPE 500
    NLHRPWVDRV ELKCPRCGGS MKRVPDVLDV WFDSGIAFYA SLGYPRDRET 550
    WERLKPVDFI VEGHDQIRGW FFSLLRSGVI GFGETPYRRV LVHGFALDEQ 600
    GREMHKSLGN YVDFEELIAK VPRDVVRFWV LQNTVWEDLR FSWKGLDLMR 650
    RDFNVIWNVY AFASIYMGLD RFDPRRTSLS SVEDSLEVED RWILSRLASL 700
    KRRYLTAMED LRIHDAARAL RSFIIEDVSH WYLRIIRRRV WEEVDTPSKR 750
    AAYATLYKVL WEWLLMAAPL IPYTAEYLYI KVFREAEERP EESIHMLDMP 800
    EPEDHLIDST LEEDMEKARM VLEAILSARN EAGLKLRRPV RRVIIAASNP 850
    GVEGSLRRLE GLIRLMANAK EIEFAPASAL EATRIYRVEP DYGRLGPKYK 900
    RDMPKVLRLI EEKGGEIGRV LAEKGVYEGE YEGLPVKLTL EDVSLKAEYP 950
    EWLKVRDTPL GLVAVDTRLT KEEVLEGLAR DIVRRIQAMR KEAGYSVEDY 1000
    VEVWIQGGED VVEAVNALKE YIKRETRALK LEVGEPPSGV EVLREWELDG 1050
    EKVVIALKRA AASG 1064
    Length:1,064
    Mass (Da):123,482
    Last modified:November 1, 1999 - v1
    Checksum:iDCB292AD67031978
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79329.1.
    PIRiE72729.
    RefSeqiNP_147189.1. NC_000854.2.
    WP_010865700.1. NC_000854.2.

    Genome annotation databases

    EnsemblBacteriaiBAA79329; BAA79329; APE_0374.
    GeneIDi1444584.
    KEGGiape:APE_0374.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79329.1 .
    PIRi E72729.
    RefSeqi NP_147189.1. NC_000854.2.
    WP_010865700.1. NC_000854.2.

    3D structure databases

    ProteinModelPortali Q9YF67.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272557.APE_0374.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA79329 ; BAA79329 ; APE_0374 .
    GeneIDi 1444584.
    KEGGi ape:APE_0374.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ERLMLHQ.

    Enzyme and pathway databases

    BioCyci APER272557:GJD6-283-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

    Entry informationi

    Entry nameiSYI_AERPE
    AccessioniPrimary (citable) accession number: Q9YF67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3