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Q9YF67

- SYI_AERPE

UniProt

Q9YF67 - SYI_AERPE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei606 – 6061ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAPER272557:GJD6-283-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:APE_0374
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
ProteomesiUP000002518: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10641064Isoleucine--tRNA ligasePRO_0000098574Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272557.APE_0374.

Structurei

3D structure databases

ProteinModelPortaliQ9YF67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6412"HIGH" regionAdd
BLAST
Motifi603 – 6075"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiERLMLHQ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9YF67-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSVPRVLEG ERYDQFALES FVKSYWEENS IYRKVKEKSS RAPRKFYFLD
60 70 80 90 100
GPPYASAKSI HIGTAWNKVV KDVVLRFYRM TGYNVWDKPG YDTHGLPIEV
110 120 130 140 150
KIEQSLGVRV KREIEEKVGV ENFIAACKRF VDENMEAMTR QFKEIGVFMD
160 170 180 190 200
WENPYVTYRD EYIESGWWLV KKAWEKGLLY KGYRVLHWCP RCETTLADYE
210 220 230 240 250
VSEYRELEDP SIYVKFPVEG REGEYLLVWT TTPWTLPANA FVMAHPDMEY
260 270 280 290 300
VKVRVGRDTL ILAKARLDHV MREAGVSEYE VLEVLKGSQL EGLRYRHPLE
310 320 330 340 350
DVVDAQRELS KWHKVVMAPE AVTPHEGSGL VHSAPGHGTI DYEIAQRIGA
360 370 380 390 400
PVVSLVDERG FMTEAAGKYS GLYFRGEANK AIVEDLRARG ALFHAATIVH
410 420 430 440 450
RYPVCWRCKT PLLLRATTQW FVAVTRLKNQ LMREAEKVEW QPGWAKTRFM
460 470 480 490 500
NMLRELRDWV ISRQRYWGIP LPVWRCSSCG YEHVVGSVEE LESMGGRRPE
510 520 530 540 550
NLHRPWVDRV ELKCPRCGGS MKRVPDVLDV WFDSGIAFYA SLGYPRDRET
560 570 580 590 600
WERLKPVDFI VEGHDQIRGW FFSLLRSGVI GFGETPYRRV LVHGFALDEQ
610 620 630 640 650
GREMHKSLGN YVDFEELIAK VPRDVVRFWV LQNTVWEDLR FSWKGLDLMR
660 670 680 690 700
RDFNVIWNVY AFASIYMGLD RFDPRRTSLS SVEDSLEVED RWILSRLASL
710 720 730 740 750
KRRYLTAMED LRIHDAARAL RSFIIEDVSH WYLRIIRRRV WEEVDTPSKR
760 770 780 790 800
AAYATLYKVL WEWLLMAAPL IPYTAEYLYI KVFREAEERP EESIHMLDMP
810 820 830 840 850
EPEDHLIDST LEEDMEKARM VLEAILSARN EAGLKLRRPV RRVIIAASNP
860 870 880 890 900
GVEGSLRRLE GLIRLMANAK EIEFAPASAL EATRIYRVEP DYGRLGPKYK
910 920 930 940 950
RDMPKVLRLI EEKGGEIGRV LAEKGVYEGE YEGLPVKLTL EDVSLKAEYP
960 970 980 990 1000
EWLKVRDTPL GLVAVDTRLT KEEVLEGLAR DIVRRIQAMR KEAGYSVEDY
1010 1020 1030 1040 1050
VEVWIQGGED VVEAVNALKE YIKRETRALK LEVGEPPSGV EVLREWELDG
1060
EKVVIALKRA AASG
Length:1,064
Mass (Da):123,482
Last modified:November 1, 1999 - v1
Checksum:iDCB292AD67031978
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000002 Genomic DNA. Translation: BAA79329.1.
PIRiE72729.
RefSeqiNP_147189.1. NC_000854.2.
WP_010865700.1. NC_000854.2.

Genome annotation databases

EnsemblBacteriaiBAA79329; BAA79329; APE_0374.
GeneIDi1444584.
KEGGiape:APE_0374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000002 Genomic DNA. Translation: BAA79329.1 .
PIRi E72729.
RefSeqi NP_147189.1. NC_000854.2.
WP_010865700.1. NC_000854.2.

3D structure databases

ProteinModelPortali Q9YF67.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272557.APE_0374.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA79329 ; BAA79329 ; APE_0374 .
GeneIDi 1444584.
KEGGi ape:APE_0374.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ERLMLHQ.

Enzyme and pathway databases

BioCyci APER272557:GJD6-283-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiSYI_AERPE
AccessioniPrimary (citable) accession number: Q9YF67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3