Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase

Gene

pyk

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

a divalent metal cation1 Publication

Enzyme regulationi

Not activated by classical allosteric effectors.

Kineticsi

    1. Vmax=53 µmol/min/mg enzyme (at 65 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6.1.1 Publication

    Temperature dependencei

    Optimum temperature is higher than 95 degrees Celsius. Still active after 120 minutes at 100 degrees Celsius.1 Publication

    Pathway:iglycolysis

    This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
    Proteins known to be involved in the 5 steps of the subpathway in this organism are:
    1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
    2. Phosphoglycerate kinase (pgk)
    3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
    4. Enolase (eno)
    5. Pyruvate kinase (pyk)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331SubstrateBy similarity
    Metal bindingi35 – 351PotassiumBy similarity
    Metal bindingi37 – 371PotassiumBy similarity
    Metal bindingi67 – 671PotassiumBy similarity
    Sitei212 – 2121Transition state stabilizerBy similarity
    Metal bindingi214 – 2141MagnesiumBy similarity
    Binding sitei237 – 2371Substrate; via amide nitrogenBy similarity
    Metal bindingi238 – 2381MagnesiumBy similarity
    Binding sitei238 – 2381Substrate; via amide nitrogenBy similarity
    Binding sitei270 – 2701SubstrateBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciAPER272557:GJD6-377-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Ordered Locus Names:APE_0489
    OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
    Taxonomic identifieri272557 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
    ProteomesiUP000002518 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 458458Pyruvate kinasePRO_0000295180Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi272557.APE_0489.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9YEU2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021558.
    KOiK00873.
    OMAiFVNSADN.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9YEU2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRGPVKIVAT VGPSSSSASI LAQMLSLGVD VARINASHGG VEQWNSMLES
    60 70 80 90 100
    LRRAEEAVGK RVGVAVDLEG PRVRTGNSEP VKLEKGDLVT LGFMEGDVPV
    110 120 130 140 150
    DARQFFETID EGDIVLLDDG KIILQVESVE GFRVKARVLE GGVLGPRKGV
    160 170 180 190 200
    VVRGKEPDLP PLSAKDRRAL EFFADKGVSH VYVSFARSAE HVEKVRTVVR
    210 220 230 240 250
    RLGLRQARIF AKIEGPSGVS RIGEIAEASD GVIIARGDLG MHYSLEELPE
    260 270 280 290 300
    IQELIVWEAR KRYKTVVLAT EFLSSMIEKP VPTRSEVVDI YQAVLQTADA
    310 320 330 340 350
    LMLTGETAIG KYPVKSVQWM AKISSRAYKK LATSPPERPR PTSTPYKLAL
    360 370 380 390 400
    GLVELAESLD SPLVVYSKTG RFAERLASFK PLKTFYVGVP SREVERVVRH
    410 420 430 440 450
    LWGAEPIVVG DYPYEAGLAK TYEKLRRENI IHGDETVVEA AWSSERGIYI

    IRVRNLEF
    Length:458
    Mass (Da):50,487
    Last modified:November 1, 1999 - v1
    Checksum:i3D8E11A37A0088E3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79454.1.
    PIRiB72745.
    RefSeqiNP_147274.1. NC_000854.2.
    WP_010865788.1. NC_000854.2.

    Genome annotation databases

    EnsemblBacteriaiBAA79454; BAA79454; APE_0489.
    GeneIDi1444673.
    KEGGiape:APE_0489.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79454.1.
    PIRiB72745.
    RefSeqiNP_147274.1. NC_000854.2.
    WP_010865788.1. NC_000854.2.

    3D structure databases

    ProteinModelPortaliQ9YEU2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi272557.APE_0489.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAA79454; BAA79454; APE_0489.
    GeneIDi1444673.
    KEGGiape:APE_0489.

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021558.
    KOiK00873.
    OMAiFVNSADN.

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.
    BioCyciAPER272557:GJD6-377-MONOMER.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.
    2. "Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea."
      Johnsen U., Hansen T., Schoenheit P.
      J. Biol. Chem. 278:25417-25427(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT, REGULATION.
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

    Entry informationi

    Entry nameiKPYK_AERPE
    AccessioniPrimary (citable) accession number: Q9YEU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: November 1, 1999
    Last modified: July 22, 2015
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.