SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9YEU2

- KPYK_AERPE

UniProt

Q9YEU2 - KPYK_AERPE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Pyruvate kinase
Gene
pyk, APE_0489
Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Divalent metal cations.1 Publication

Enzyme regulationi

Not activated by classical allosteric effectors.

Kineticsi

    Vmax=53 µmol/min/mg enzyme (at 65 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6.1.

    Temperature dependencei

    Optimum temperature is higher than 95 degrees Celsius. Still active after 120 minutes at 100 degrees Celsius.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331Substrate By similarity
    Metal bindingi35 – 351Potassium By similarity
    Metal bindingi37 – 371Potassium By similarity
    Metal bindingi67 – 671Potassium By similarity
    Sitei212 – 2121Transition state stabilizer By similarity
    Metal bindingi214 – 2141Magnesium By similarity
    Binding sitei237 – 2371Substrate; via amide nitrogen By similarity
    Metal bindingi238 – 2381Magnesium By similarity
    Binding sitei238 – 2381Substrate; via amide nitrogen By similarity
    Binding sitei270 – 2701Substrate By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC
    Complete GO annotation...

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciAPER272557:GJD6-377-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Ordered Locus Names:APE_0489
    OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
    Taxonomic identifieri272557 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
    ProteomesiUP000002518: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 458458Pyruvate kinase
    PRO_0000295180Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi272557.APE_0489.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9YEU2.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021558.
    KOiK00873.
    OMAiERDPIYR.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9YEU2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGPVKIVAT VGPSSSSASI LAQMLSLGVD VARINASHGG VEQWNSMLES    50
    LRRAEEAVGK RVGVAVDLEG PRVRTGNSEP VKLEKGDLVT LGFMEGDVPV 100
    DARQFFETID EGDIVLLDDG KIILQVESVE GFRVKARVLE GGVLGPRKGV 150
    VVRGKEPDLP PLSAKDRRAL EFFADKGVSH VYVSFARSAE HVEKVRTVVR 200
    RLGLRQARIF AKIEGPSGVS RIGEIAEASD GVIIARGDLG MHYSLEELPE 250
    IQELIVWEAR KRYKTVVLAT EFLSSMIEKP VPTRSEVVDI YQAVLQTADA 300
    LMLTGETAIG KYPVKSVQWM AKISSRAYKK LATSPPERPR PTSTPYKLAL 350
    GLVELAESLD SPLVVYSKTG RFAERLASFK PLKTFYVGVP SREVERVVRH 400
    LWGAEPIVVG DYPYEAGLAK TYEKLRRENI IHGDETVVEA AWSSERGIYI 450
    IRVRNLEF 458
    Length:458
    Mass (Da):50,487
    Last modified:November 1, 1999 - v1
    Checksum:i3D8E11A37A0088E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79454.1.
    PIRiB72745.
    RefSeqiNP_147274.1. NC_000854.2.
    WP_010865788.1. NC_000854.2.

    Genome annotation databases

    EnsemblBacteriaiBAA79454; BAA79454; APE_0489.
    GeneIDi1444673.
    KEGGiape:APE_0489.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79454.1 .
    PIRi B72745.
    RefSeqi NP_147274.1. NC_000854.2.
    WP_010865788.1. NC_000854.2.

    3D structure databases

    ProteinModelPortali Q9YEU2.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 272557.APE_0489.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA79454 ; BAA79454 ; APE_0489 .
    GeneIDi 1444673.
    KEGGi ape:APE_0489.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021558.
    KOi K00873.
    OMAi ERDPIYR.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci APER272557:GJD6-377-MONOMER.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.
    2. "Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea."
      Johnsen U., Hansen T., Schoenheit P.
      J. Biol. Chem. 278:25417-25427(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT, REGULATION.
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

    Entry informationi

    Entry nameiKPYK_AERPE
    AccessioniPrimary (citable) accession number: Q9YEU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: November 1, 1999
    Last modified: September 3, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi