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Q9YEU2

- KPYK_AERPE

UniProt

Q9YEU2 - KPYK_AERPE

Protein

Pyruvate kinase

Gene

pyk

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Divalent metal cations.1 Publication

    Enzyme regulationi

    Not activated by classical allosteric effectors.

    Kineticsi

      Vmax=53 µmol/min/mg enzyme (at 65 degrees Celsius)1 Publication

      pH dependencei

      Optimum pH is 6.1.1 Publication

      Temperature dependencei

      Optimum temperature is higher than 95 degrees Celsius. Still active after 120 minutes at 100 degrees Celsius.1 Publication

      Pathwayi

      Sites

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Binding sitei33 – 331SubstrateBy similarity
      Metal bindingi35 – 351PotassiumBy similarity
      Metal bindingi37 – 371PotassiumBy similarity
      Metal bindingi67 – 671PotassiumBy similarity
      Sitei212 – 2121Transition state stabilizerBy similarity
      Metal bindingi214 – 2141MagnesiumBy similarity
      Binding sitei237 – 2371Substrate; via amide nitrogenBy similarity
      Metal bindingi238 – 2381MagnesiumBy similarity
      Binding sitei238 – 2381Substrate; via amide nitrogenBy similarity
      Binding sitei270 – 2701SubstrateBy similarity

      GO - Molecular functioni

      1. ATP binding Source: UniProtKB-KW
      2. magnesium ion binding Source: InterPro
      3. potassium ion binding Source: InterPro
      4. pyruvate kinase activity Source: UniProtKB-EC

      GO - Biological processi

      1. glycolytic process Source: UniProtKB-UniPathway

      Keywords - Molecular functioni

      Kinase, Transferase

      Keywords - Biological processi

      Glycolysis

      Keywords - Ligandi

      ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

      Enzyme and pathway databases

      BioCyciAPER272557:GJD6-377-MONOMER.
      UniPathwayiUPA00109; UER00188.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Pyruvate kinase (EC:2.7.1.40)
      Short name:
      PK
      Gene namesi
      Name:pyk
      Ordered Locus Names:APE_0489
      OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
      Taxonomic identifieri272557 [NCBI]
      Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
      ProteomesiUP000002518: Chromosome

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 458458Pyruvate kinasePRO_0000295180Add
      BLAST

      Interactioni

      Subunit structurei

      Homotetramer.1 Publication

      Protein-protein interaction databases

      STRINGi272557.APE_0489.

      Structurei

      3D structure databases

      ProteinModelPortaliQ9YEU2.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Sequence similaritiesi

      Belongs to the pyruvate kinase family.Curated

      Phylogenomic databases

      eggNOGiCOG0469.
      HOGENOMiHOG000021558.
      KOiK00873.
      OMAiERDPIYR.

      Family and domain databases

      Gene3Di2.40.33.10. 1 hit.
      3.20.20.60. 2 hits.
      3.40.1380.20. 1 hit.
      InterProiIPR001697. Pyr_Knase.
      IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
      IPR011037. Pyrv_Knase-like_insert_dom.
      IPR015794. Pyrv_Knase_a/b.
      IPR015793. Pyrv_Knase_brl.
      IPR015795. Pyrv_Knase_C.
      IPR015806. Pyrv_Knase_insert_dom.
      [Graphical view]
      PANTHERiPTHR11817. PTHR11817. 1 hit.
      PfamiPF00224. PK. 1 hit.
      PF02887. PK_C. 1 hit.
      [Graphical view]
      PRINTSiPR01050. PYRUVTKNASE.
      SUPFAMiSSF50800. SSF50800. 1 hit.
      SSF51621. SSF51621. 2 hits.
      SSF52935. SSF52935. 1 hit.
      TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

      Sequencei

      Sequence statusi: Complete.

      Q9YEU2-1 [UniParc]FASTAAdd to Basket

      « Hide

      MRGPVKIVAT VGPSSSSASI LAQMLSLGVD VARINASHGG VEQWNSMLES    50
      LRRAEEAVGK RVGVAVDLEG PRVRTGNSEP VKLEKGDLVT LGFMEGDVPV 100
      DARQFFETID EGDIVLLDDG KIILQVESVE GFRVKARVLE GGVLGPRKGV 150
      VVRGKEPDLP PLSAKDRRAL EFFADKGVSH VYVSFARSAE HVEKVRTVVR 200
      RLGLRQARIF AKIEGPSGVS RIGEIAEASD GVIIARGDLG MHYSLEELPE 250
      IQELIVWEAR KRYKTVVLAT EFLSSMIEKP VPTRSEVVDI YQAVLQTADA 300
      LMLTGETAIG KYPVKSVQWM AKISSRAYKK LATSPPERPR PTSTPYKLAL 350
      GLVELAESLD SPLVVYSKTG RFAERLASFK PLKTFYVGVP SREVERVVRH 400
      LWGAEPIVVG DYPYEAGLAK TYEKLRRENI IHGDETVVEA AWSSERGIYI 450
      IRVRNLEF 458
      Length:458
      Mass (Da):50,487
      Last modified:November 1, 1999 - v1
      Checksum:i3D8E11A37A0088E3
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      BA000002 Genomic DNA. Translation: BAA79454.1.
      PIRiB72745.
      RefSeqiNP_147274.1. NC_000854.2.
      WP_010865788.1. NC_000854.2.

      Genome annotation databases

      EnsemblBacteriaiBAA79454; BAA79454; APE_0489.
      GeneIDi1444673.
      KEGGiape:APE_0489.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      BA000002 Genomic DNA. Translation: BAA79454.1 .
      PIRi B72745.
      RefSeqi NP_147274.1. NC_000854.2.
      WP_010865788.1. NC_000854.2.

      3D structure databases

      ProteinModelPortali Q9YEU2.
      ModBasei Search...
      MobiDBi Search...

      Protein-protein interaction databases

      STRINGi 272557.APE_0489.

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Genome annotation databases

      EnsemblBacteriai BAA79454 ; BAA79454 ; APE_0489 .
      GeneIDi 1444673.
      KEGGi ape:APE_0489.

      Phylogenomic databases

      eggNOGi COG0469.
      HOGENOMi HOG000021558.
      KOi K00873.
      OMAi ERDPIYR.

      Enzyme and pathway databases

      UniPathwayi UPA00109 ; UER00188 .
      BioCyci APER272557:GJD6-377-MONOMER.

      Family and domain databases

      Gene3Di 2.40.33.10. 1 hit.
      3.20.20.60. 2 hits.
      3.40.1380.20. 1 hit.
      InterProi IPR001697. Pyr_Knase.
      IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
      IPR011037. Pyrv_Knase-like_insert_dom.
      IPR015794. Pyrv_Knase_a/b.
      IPR015793. Pyrv_Knase_brl.
      IPR015795. Pyrv_Knase_C.
      IPR015806. Pyrv_Knase_insert_dom.
      [Graphical view ]
      PANTHERi PTHR11817. PTHR11817. 1 hit.
      Pfami PF00224. PK. 1 hit.
      PF02887. PK_C. 1 hit.
      [Graphical view ]
      PRINTSi PR01050. PYRUVTKNASE.
      SUPFAMi SSF50800. SSF50800. 1 hit.
      SSF51621. SSF51621. 2 hits.
      SSF52935. SSF52935. 1 hit.
      TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
      ProtoNeti Search...

      Publicationsi

      1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.
      2. "Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea."
        Johnsen U., Hansen T., Schoenheit P.
        J. Biol. Chem. 278:25417-25427(2003) [PubMed] [Europe PMC] [Abstract]
        Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT, REGULATION.
        Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

      Entry informationi

      Entry nameiKPYK_AERPE
      AccessioniPrimary (citable) accession number: Q9YEU2
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: July 10, 2007
      Last sequence update: November 1, 1999
      Last modified: October 1, 2014
      This is version 77 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Reference proteome

      Documents

      1. PATHWAY comments
        Index of metabolic and biosynthesis pathways
      2. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3