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Q9YES0 (RTCA_AERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:APE_0511.1
OrganismAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) [Complete proteome] [HAMAP]
Taxonomic identifier272557 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_0000156422

Regions

Nucleotide binding295 – 2984ATP By similarity

Sites

Active site3211Tele-AMP-histidine intermediate By similarity
Binding site1051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9YES0 [UniParc].

Last modified June 26, 2007. Version 2.
Checksum: B2E8BFE6CF844708

FASTA36138,290
        10         20         30         40         50         60 
MGSGEWVVVD GSMGEGGGQI LRTAVALAAV LGRPLRIVNI RAKRRPPGLR PQHLTAVRAV 

        70         80         90        100        110        120 
AAISGGRLRG AEVGSTSLEF TPGRVRGGRY RFDVGTAGSV ALIIQALAPL LAYSDSPVEV 

       130        140        150        160        170        180 
ELTGGTDVPM APTIDYMREV FASILAMLGY EIEIRVLRRG HYPRGGGRVV VRVPDPPGGF 

       190        200        210        220        230        240 
RARSFVERGP LKGVYVRSHA VKLPGSIAER QARSAASLVR ERLGVDPIVE IEAYKPHRDP 

       250        260        270        280        290        300 
HLGPGTGVLV WAVFGETVMG GDSIGKKGKP AEVVGREAAE SLLEDMATGA ALDRHMSDMA 

       310        320        330        340        350        360 
PVYLALAGGV STVFGARLTG HASTILELLR IMVDGFEYRI LEGGLERPFK AELRGAGVSP 


R 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000002 Genomic DNA. Translation: BAA79476.2.
PIRH72747.
RefSeqNP_147291.2. NC_000854.2.

3D structure databases

ProteinModelPortalQ9YES0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272557.APE_0511.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA79476; BAA79476; APE_0511.1.
GeneID1444690.
KEGGape:APE_0511.1.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0430.
HOGENOMHOG000015264.
KOK01974.
ProtClustDBPRK04204.

Enzyme and pathway databases

BioCycAPER272557:GJD6-391-MONOMER.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_AERPE
AccessionPrimary (citable) accession number: Q9YES0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 26, 2007
Last modified: February 19, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families