Reviewed, UniProtKB/Swiss-Prot Q9YEF2 (SAHH_AERPE)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	APE_0624.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000117000
Regions
	Region	178 – 345	168	NAD binding By similarity
Sites
	Binding site	55	1	Substrate By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	185	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YEF2-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: E145E95F5DDE9880	FASTA	416	45,919
	10 20 30 40 50 60 MAQGFKVRDL SLAGEGRMQI EWAERHMPVL MRLRSSMGGD KPLSGVRVAA CLHVTKETAV 70 80 90 100 110 120 LVETLRKWGA EVYLAPSNPL STQDEVAAAL AEAGIGVFAW RGMTPEEYKW ALSTVAGREP 130 140 150 160 170 180 DIVIDDGADL HVLLHEEMRS VGEKVWGGTE ETTTGVIRLR ALEREGRLLY PVIAVNDALT 190 200 210 220 230 240 KFMFDNRYGT GQSTVDGVLR ATNILIAGKT VVVAGYGWVG RGIAARFRGM GAKVVVTEVD 250 260 270 280 290 300 PVRALEAAMD GFTVTTMDEA ASLGDVFITA TGNINVIDAR HMEKMKDGAI LANAGHFNVE 310 320 330 340 350 360 INVAALEEMS VSKRRVRRYL DEYRLPDGRR LYLIGEGRLV NLVAAEGHPS EVMDMSFSNQ 370 380 390 400 410 ALAVLKIAGE RGRLEKRVHR VERIQDEMVA RLKLETMGVR IDSLTEEQKL YLQKWK

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA79594.2.
PIR	B72649.
RefSeq	NP_147374.2.
3D structure databases
HSSP	HSSP built from PDB template 1LI4 based on UniProtKB P23526.
ModBase	Search...
Genome annotation databases
GeneID	1444774.
GenomeReviews	Gene locus APE_0624.1 in contig BA000002_GR.
KEGG	ape:APE_0624.1.
NMPDR	fig|272557.1.peg.482.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YEF2.
OMA	IRLKAMA.
Enzyme and pathway databases
BRENDA	3.3.1.1. 256344.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_AERPE

Accession

Primary (citable) accession number: Q9YEF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 6, 2002
Last modified:	November 3, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)