Phosphoenolpyruvate synthase - Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate synthase
Short name=PEP synthase
EC=2.7.9.2

Alternative name(s):
Pyruvate, water dikinase

Gene names

Name:	ppsA
Ordered Locus Names:	APE_0650.1

Organism

Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) [Complete proteome] [HAMAP]

Taxonomic identifier

272557 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate By similarity.
Catalytic activity	ATP + pyruvate + H₂O = AMP + phosphoenolpyruvate + phosphate.
Cofactor	Magnesium By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Domain	The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 820

820

Phosphoenolpyruvate synthase

PRO_0000147040

Sites

Active site

438

1

Tele-phosphohistidine intermediate By similarity

Active site

762

1

Proton donor By similarity

Metal binding

689

1

Magnesium By similarity

Metal binding

713

1

Magnesium By similarity

Binding site

539

1

Substrate By similarity

Binding site

587

1

Substrate By similarity

Binding site

689

1

Substrate By similarity

Binding site

710

1

Substrate; via carbonyl oxygen By similarity

Binding site

711

1

Substrate; via amide nitrogen By similarity

Binding site

712

1

Substrate By similarity

Binding site

713

1

Substrate; via amide nitrogen By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9YEC5 [UniParc].

Last modified June 26, 2007. Version 2.
Checksum: 7E3B54DABCCA3851
Show »

FASTA

820

91,854

        10         20         30         40         50         60 
MERRLILWLD EISKDLLPLV GGKAAGLGEM IKAGIPVPPG FVVTSEAYRR FVFETGIAGF 

        70         80         90        100        110        120 
IKHILEETIV SGRPEEYEKA SELIRSKFVR TPMPPYLRRA IVDAYRKLGT LVGVEEPRVA 

       130        140        150        160        170        180 
VRSSATVEDL PEASFAGQQE TYLNVKGEEE VVEKVKTAWA SLWTARALSY RDSLNIDHET 

       190        200        210        220        230        240 
ALMAVVVQKM VSSRSSGVMF TIHPVTGEED KIVIESIWGL GEYIVGGKVT PDRFVVSKSD 

       250        260        270        280        290        300 
LEILEVRISR KDKALFYDPD LNENVEIKIP ESGEELEDLR RKHPAVAEVV EKYGIRPDAP 

       310        320        330        340        350        360 
SLSEKEVKEL ARLAIKVENH FARPMDIEWA IDFELSPPEN ILLLQARPET VWSRKKEEAE 

       370        380        390        400        410        420 
AKPPEEEAVP EGEVLVRGVP ASPGVASGRV KVALTVEEAA KKMEKGDVLV TKMTDPDWVP 

       430        440        450        460        470        480 
YMKMASAIVT DEGGMTAHAA IVARELGIPA VVGTGDATKK LKDGMLVTVD GSRGVVYAGA 

       490        500        510        520        530        540 
VKTEEAREEE ARPELGAAVA EVLSEVYPVT ATKIYMNLGH PEEIDRYKHL PFEGIGLMRI 

       550        560        570        580        590        600 
EFIISSWIGY HPMYLIEQGR GVYFIDKLAE GVAKVASAIY PRPVVVRFSD FKTNEYRRLK 

       610        620        630        640        650        660 
GGEKYETLDE RNPMIGWRGV SRYIHPNYEP AFRLEVRAIK KVREEWGLKN VWVMFPFVRT 

       670        680        690        700        710        720 
TWELERALKI MEEEGLSRGR DFKVWIMVEV PSTVFLADEF AKMVDGFSIG SNDLTQLVLG 

       730        740        750        760        770        780 
VDRDSGFLAK MGYFDERDPA VLRSIEILIE KAHSQGATVS ICGQGPSVYP ELVEFLVRRG 

       790        800        810        820 
IDSISVNPDA VVRVRRQVAS IERRIMLERL EELGSRLSRL

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References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1."
Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.

Kikuchi H.
DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000002 Genomic DNA. Translation: BAA79621.2.
PIR	E72652.
RefSeq	NP_147390.2. NC_000854.2.
3D structure databases
ProteinModelPortal	Q9YEC5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1444791.
GenomeReviews	Gene locus APE_0650.1 in contig BA000002_GR.
KEGG	ape:APE_0650.1.
NMPDR	fig\|272557.1.peg.498.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284863.
ProtClustDB	PRK06464.
Enzyme and pathway databases
BioCyc	APER272557:APE0650-MONOMER.
Family and domain databases
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR008279. PEP-util_enz_mobile_dom. IPR006319. PEP_synth. IPR018274. PEP_util_AS. IPR000121. PEP_util_C. IPR023151. PEP_util_CS. IPR002192. PPDK_PEP-bd. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K01007.
Pfam	PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. PF01326. PPDK_N. 1 hit. [Graphical view]
PIRSF	PIRSF000854. PEP_synthase. 1 hit.
SUPFAM	SSF52009. PEP_mobile. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01418. PEP_synth. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPSA_AERPE

Accession

Primary (citable) accession number: Q9YEC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 26, 2007
Last modified:	January 25, 2012
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents