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Q9YE60

- AGOG_AERPE

UniProt

Q9YE60 - AGOG_AERPE

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Protein

N-glycosylase/DNA lyase

Gene

APE_0710.1

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 3518-oxoguanineUniRule annotation
Binding sitei62 – 6218-oxoguanine; via carbonyl oxygenUniRule annotation
Binding sitei73 – 7318-oxoguanineUniRule annotation
Active sitei160 – 1601Schiff-base intermediate with DNAUniRule annotation
Binding sitei164 – 16418-oxoguanineUniRule annotation
Binding sitei189 – 18918-oxoguanine; via carbonyl oxygenUniRule annotation
Active sitei191 – 1911UniRule annotation
Binding sitei238 – 23818-oxoguanineUniRule annotation
Binding sitei242 – 24218-oxoguanineUniRule annotation

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair

Enzyme and pathway databases

BioCyciAPER272557:GJD6-517-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyaseUniRule annotation
Alternative name(s):
8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
AGOGUniRule annotation
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyaseUniRule annotation
Gene namesi
Ordered Locus Names:APE_0710.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
ProteomesiUP000002518: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288N-glycosylase/DNA lyasePRO_0000185108Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272557.APE_0710.1.

Structurei

3D structure databases

ProteinModelPortaliQ9YE60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 20370Helix-hairpin-helixAdd
BLAST

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

Sequence similaritiesi

Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

Phylogenomic databases

eggNOGiCOG4047.
HOGENOMiHOG000033850.
KOiK01741.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_01168. AGOG.
InterProiIPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view]
PfamiPF09171. DUF1886. 1 hit.
[Graphical view]
PIRSFiPIRSF008955. AGOG. 1 hit.
SUPFAMiSSF48150. SSF48150. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9YE60-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQRVRWERV ERVAEAFSRL SIGEVLGFEE QVDPQYKLVS RLAGEIGAGK
60 70 80 90 100
AALSALLVGL ASYRLAMRGE EWWLCFYRHM RSSLPRAEGL RGVLRAVEGF
110 120 130 140 150
LTSCSGAAIG REAKLRRVRR AASAAEVLGE VLDNPLVLVE RPSEVLEALR
160 170 180 190 200
VALGEKGFRK TTVFSVKIAY YAVRPLAGRK PLTLDVPIPV DVRVACASIS
210 220 230 240 250
SEMVEAPSYR EVVARPEAAQ RAWGRVARSS GIPVLHIDSI LWVTGWAPRE
260 270 280
LPPGEAREAV AGILSRALDR GKAVLLASEL VRRPCPGG
Length:288
Mass (Da):31,443
Last modified:June 26, 2007 - v2
Checksum:iA57E5A1F0A2786C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA79686.2.
PIRiF72660.
RefSeqiNP_147435.2. NC_000854.2.
WP_010865919.1. NC_000854.2.

Genome annotation databases

EnsemblBacteriaiBAA79686; BAA79686; APE_0710.1.
GeneIDi1444838.
KEGGiape:APE_0710.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA79686.2 .
PIRi F72660.
RefSeqi NP_147435.2. NC_000854.2.
WP_010865919.1. NC_000854.2.

3D structure databases

ProteinModelPortali Q9YE60.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272557.APE_0710.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA79686 ; BAA79686 ; APE_0710.1 .
GeneIDi 1444838.
KEGGi ape:APE_0710.1.

Phylogenomic databases

eggNOGi COG4047.
HOGENOMi HOG000033850.
KOi K01741.

Enzyme and pathway databases

BioCyci APER272557:GJD6-517-MONOMER.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_01168. AGOG.
InterProi IPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view ]
Pfami PF09171. DUF1886. 1 hit.
[Graphical view ]
PIRSFi PIRSF008955. AGOG. 1 hit.
SUPFAMi SSF48150. SSF48150. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiAGOG_AERPE
AccessioniPrimary (citable) accession number: Q9YE60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 26, 2007
Last modified: November 26, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3