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Q9YE60

- AGOG_AERPE

UniProt

Q9YE60 - AGOG_AERPE

Protein

N-glycosylase/DNA lyase

Gene

APE_0710.1

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 2 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei35 – 3518-oxoguanineUniRule annotation
    Binding sitei62 – 6218-oxoguanine; via carbonyl oxygenUniRule annotation
    Binding sitei73 – 7318-oxoguanineUniRule annotation
    Active sitei160 – 1601Schiff-base intermediate with DNAUniRule annotation
    Binding sitei164 – 16418-oxoguanineUniRule annotation
    Binding sitei189 – 18918-oxoguanine; via carbonyl oxygenUniRule annotation
    Active sitei191 – 1911UniRule annotation
    Binding sitei238 – 23818-oxoguanineUniRule annotation
    Binding sitei242 – 24218-oxoguanineUniRule annotation

    GO - Molecular functioni

    1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
    2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. base-excision repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA excision, DNA repair

    Enzyme and pathway databases

    BioCyciAPER272557:GJD6-517-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-glycosylase/DNA lyaseUniRule annotation
    Alternative name(s):
    8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
    AGOGUniRule annotation
    DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
    Short name:
    AP lyaseUniRule annotation
    Gene namesi
    Ordered Locus Names:APE_0710.1
    OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
    Taxonomic identifieri272557 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
    ProteomesiUP000002518: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288N-glycosylase/DNA lyasePRO_0000185108Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272557.APE_0710.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9YE60.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni134 – 20370Helix-hairpin-helixAdd
    BLAST

    Domaini

    Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

    Sequence similaritiesi

    Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG4047.
    HOGENOMiHOG000033850.
    KOiK01741.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_01168. AGOG.
    InterProiIPR011257. DNA_glycosylase.
    IPR023170. HTH_base_excis_C.
    IPR015254. N-Glyclase/DNA_lyase-like_arc.
    IPR016544. N-Glyclase/DNA_lyase_arc.
    [Graphical view]
    PfamiPF09171. DUF1886. 1 hit.
    [Graphical view]
    PIRSFiPIRSF008955. AGOG. 1 hit.
    SUPFAMiSSF48150. SSF48150. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9YE60-1 [UniParc]FASTAAdd to Basket

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    MAQRVRWERV ERVAEAFSRL SIGEVLGFEE QVDPQYKLVS RLAGEIGAGK    50
    AALSALLVGL ASYRLAMRGE EWWLCFYRHM RSSLPRAEGL RGVLRAVEGF 100
    LTSCSGAAIG REAKLRRVRR AASAAEVLGE VLDNPLVLVE RPSEVLEALR 150
    VALGEKGFRK TTVFSVKIAY YAVRPLAGRK PLTLDVPIPV DVRVACASIS 200
    SEMVEAPSYR EVVARPEAAQ RAWGRVARSS GIPVLHIDSI LWVTGWAPRE 250
    LPPGEAREAV AGILSRALDR GKAVLLASEL VRRPCPGG 288
    Length:288
    Mass (Da):31,443
    Last modified:June 26, 2007 - v2
    Checksum:iA57E5A1F0A2786C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79686.2.
    PIRiF72660.
    RefSeqiNP_147435.2. NC_000854.2.

    Genome annotation databases

    EnsemblBacteriaiBAA79686; BAA79686; APE_0710.1.
    GeneIDi1444838.
    KEGGiape:APE_0710.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79686.2 .
    PIRi F72660.
    RefSeqi NP_147435.2. NC_000854.2.

    3D structure databases

    ProteinModelPortali Q9YE60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272557.APE_0710.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA79686 ; BAA79686 ; APE_0710.1 .
    GeneIDi 1444838.
    KEGGi ape:APE_0710.1.

    Phylogenomic databases

    eggNOGi COG4047.
    HOGENOMi HOG000033850.
    KOi K01741.

    Enzyme and pathway databases

    BioCyci APER272557:GJD6-517-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_01168. AGOG.
    InterProi IPR011257. DNA_glycosylase.
    IPR023170. HTH_base_excis_C.
    IPR015254. N-Glyclase/DNA_lyase-like_arc.
    IPR016544. N-Glyclase/DNA_lyase_arc.
    [Graphical view ]
    Pfami PF09171. DUF1886. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF008955. AGOG. 1 hit.
    SUPFAMi SSF48150. SSF48150. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

    Entry informationi

    Entry nameiAGOG_AERPE
    AccessioniPrimary (citable) accession number: Q9YE60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3