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Protein

N-glycosylase/DNA lyase

Gene

APE_0710.1

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei358-oxoguanineUniRule annotation1
Binding sitei628-oxoguanine; via carbonyl oxygenUniRule annotation1
Binding sitei738-oxoguanineUniRule annotation1
Active sitei160Schiff-base intermediate with DNAUniRule annotation1
Binding sitei1648-oxoguanineUniRule annotation1
Binding sitei1898-oxoguanine; via carbonyl oxygenUniRule annotation1
Active sitei191UniRule annotation1
Binding sitei2388-oxoguanineUniRule annotation1
Binding sitei2428-oxoguanineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
Biological processDNA damage, DNA excision, DNA repair

Enzyme and pathway databases

BioCyciAPER272557:GJD6-517-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyaseUniRule annotation
Alternative name(s):
8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
AGOGUniRule annotation
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyaseUniRule annotation
Gene namesi
Ordered Locus Names:APE_0710.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi
  • UP000002518 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001851081 – 288N-glycosylase/DNA lyaseAdd BLAST288

Proteomic databases

PRIDEiQ9YE60

Interactioni

Protein-protein interaction databases

STRINGi272557.APE_0710.1

Structurei

3D structure databases

ProteinModelPortaliQ9YE60
SMRiQ9YE60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 203Helix-hairpin-helixAdd BLAST70

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

Sequence similaritiesi

Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04144 Archaea
COG4047 LUCA
HOGENOMiHOG000033850
KOiK01741
OrthoDBiPOG093Z09FD

Family and domain databases

Gene3Di1.10.1670.10, 2 hits
HAMAPiMF_01168 AGOG, 1 hit
InterProiView protein in InterPro
IPR016544 AGOG
IPR015254 AGOG-like
IPR011257 DNA_glycosylase
IPR023170 HTH_base_excis_C
PfamiView protein in Pfam
PF09171 AGOG, 1 hit
PIRSFiPIRSF008955 AGOG, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit

Sequencei

Sequence statusi: Complete.

Q9YE60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQRVRWERV ERVAEAFSRL SIGEVLGFEE QVDPQYKLVS RLAGEIGAGK
60 70 80 90 100
AALSALLVGL ASYRLAMRGE EWWLCFYRHM RSSLPRAEGL RGVLRAVEGF
110 120 130 140 150
LTSCSGAAIG REAKLRRVRR AASAAEVLGE VLDNPLVLVE RPSEVLEALR
160 170 180 190 200
VALGEKGFRK TTVFSVKIAY YAVRPLAGRK PLTLDVPIPV DVRVACASIS
210 220 230 240 250
SEMVEAPSYR EVVARPEAAQ RAWGRVARSS GIPVLHIDSI LWVTGWAPRE
260 270 280
LPPGEAREAV AGILSRALDR GKAVLLASEL VRRPCPGG
Length:288
Mass (Da):31,443
Last modified:June 26, 2007 - v2
Checksum:iA57E5A1F0A2786C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA Translation: BAA79686.2
PIRiF72660

Genome annotation databases

EnsemblBacteriaiBAA79686; BAA79686; APE_0710.1
KEGGiape:APE_0710.1

Similar proteinsi

Entry informationi

Entry nameiAGOG_AERPE
AccessioniPrimary (citable) accession number: Q9YE60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 26, 2007
Last modified: May 23, 2018
This is version 74 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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