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Q9YE60

- AGOG_AERPE

UniProt

Q9YE60 - AGOG_AERPE

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Protein

N-glycosylase/DNA lyase

Gene
APE_0710.1
Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 3518-oxoguanine By similarity
Binding sitei62 – 6218-oxoguanine; via carbonyl oxygen By similarity
Binding sitei73 – 7318-oxoguanine By similarity
Active sitei160 – 1601Schiff-base intermediate with DNA By similarity
Binding sitei164 – 16418-oxoguanine By similarity
Binding sitei189 – 18918-oxoguanine; via carbonyl oxygen By similarity
Active sitei191 – 1911 Reviewed prediction
Binding sitei238 – 23818-oxoguanine By similarity
Binding sitei242 – 24218-oxoguanine By similarity

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair

Enzyme and pathway databases

BioCyciAPER272557:GJD6-517-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyase
Alternative name(s):
8-oxoguanine DNA glycosylase (EC:3.2.2.-)
AGOG
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Ordered Locus Names:APE_0710.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
ProteomesiUP000002518: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288N-glycosylase/DNA lyaseUniRule annotationPRO_0000185108Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272557.APE_0710.1.

Structurei

3D structure databases

ProteinModelPortaliQ9YE60.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 20370Helix-hairpin-helixUniRule annotationAdd
BLAST

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4047.
HOGENOMiHOG000033850.
KOiK01741.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_01168. AGOG.
InterProiIPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view]
PfamiPF09171. DUF1886. 1 hit.
[Graphical view]
PIRSFiPIRSF008955. AGOG. 1 hit.
SUPFAMiSSF48150. SSF48150. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9YE60-1 [UniParc]FASTAAdd to Basket

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MAQRVRWERV ERVAEAFSRL SIGEVLGFEE QVDPQYKLVS RLAGEIGAGK    50
AALSALLVGL ASYRLAMRGE EWWLCFYRHM RSSLPRAEGL RGVLRAVEGF 100
LTSCSGAAIG REAKLRRVRR AASAAEVLGE VLDNPLVLVE RPSEVLEALR 150
VALGEKGFRK TTVFSVKIAY YAVRPLAGRK PLTLDVPIPV DVRVACASIS 200
SEMVEAPSYR EVVARPEAAQ RAWGRVARSS GIPVLHIDSI LWVTGWAPRE 250
LPPGEAREAV AGILSRALDR GKAVLLASEL VRRPCPGG 288
Length:288
Mass (Da):31,443
Last modified:June 26, 2007 - v2
Checksum:iA57E5A1F0A2786C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000002 Genomic DNA. Translation: BAA79686.2.
PIRiF72660.
RefSeqiNP_147435.2. NC_000854.2.

Genome annotation databases

EnsemblBacteriaiBAA79686; BAA79686; APE_0710.1.
GeneIDi1444838.
KEGGiape:APE_0710.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000002 Genomic DNA. Translation: BAA79686.2 .
PIRi F72660.
RefSeqi NP_147435.2. NC_000854.2.

3D structure databases

ProteinModelPortali Q9YE60.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272557.APE_0710.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA79686 ; BAA79686 ; APE_0710.1 .
GeneIDi 1444838.
KEGGi ape:APE_0710.1.

Phylogenomic databases

eggNOGi COG4047.
HOGENOMi HOG000033850.
KOi K01741.

Enzyme and pathway databases

BioCyci APER272557:GJD6-517-MONOMER.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_01168. AGOG.
InterProi IPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view ]
Pfami PF09171. DUF1886. 1 hit.
[Graphical view ]
PIRSFi PIRSF008955. AGOG. 1 hit.
SUPFAMi SSF48150. SSF48150. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiAGOG_AERPE
AccessioniPrimary (citable) accession number: Q9YE60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 26, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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