Reviewed, UniProtKB/Swiss-Prot Q9YE60 (AGOG_AERPE)

Names and origin

Protein names

Recommended name:
    N-glycosylase/DNA lyase
Alternative name(s):
    AGOG
    8-oxoguanine DNA glycosylase
    EC=3.2.2.-
    DNA-(apurinic or apyrimidinic site) lyase
      Short name=AP lyase
    EC=4.2.99.18

Gene names

Ordered Locus Names:

APE_0710.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity. HAMAP MF_01168
Catalytic activity	The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP MF_01168
Domain	Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif). HAMAP MF_01168
Sequence similarities	Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair
Molecular function	Hydrolase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA-(apurinic or apyrimidinic site) lyase activity Inferred from electronic annotation. Source: HAMAP oxidized base lesion DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	N-glycosylase/DNA lyase HAMAP MF_01168		PRO_0000185108
Regions
	Region	134 – 203	70	Helix-hairpin-helix HAMAP MF_01168
Sites
	Active site	160	1	Schiff-base intermediate with DNA By similarity
	Active site	191	1	Potential
	Binding site	35	1	8-oxoguanine By similarity
	Binding site	62	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	73	1	8-oxoguanine By similarity
	Binding site	164	1	8-oxoguanine By similarity
	Binding site	189	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	238	1	8-oxoguanine By similarity
	Binding site	242	1	8-oxoguanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YE60-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: A57E5A1F0A2786C3	FASTA	288	31,443
	10 20 30 40 50 60 MAQRVRWERV ERVAEAFSRL SIGEVLGFEE QVDPQYKLVS RLAGEIGAGK AALSALLVGL 70 80 90 100 110 120 ASYRLAMRGE EWWLCFYRHM RSSLPRAEGL RGVLRAVEGF LTSCSGAAIG REAKLRRVRR 130 140 150 160 170 180 AASAAEVLGE VLDNPLVLVE RPSEVLEALR VALGEKGFRK TTVFSVKIAY YAVRPLAGRK 190 200 210 220 230 240 PLTLDVPIPV DVRVACASIS SEMVEAPSYR EVVARPEAAQ RAWGRVARSS GIPVLHIDSI 250 260 270 280 LWVTGWAPRE LPPGEAREAV AGILSRALDR GKAVLLASEL VRRPCPGG

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000002 Genomic DNA. Translation: BAA79686.2.
PIR	F72660.
RefSeq	NP_147435.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1444838.
GenomeReviews	Gene locus APE_0710.1 in contig BA000002_GR.
KEGG	ape:APE_0710.1.
NMPDR	fig|272557.1.peg.543.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	APER272557:APE0710-MONOMER.
BRENDA	4.2.99.18. 256344.
Family and domain databases
HAMAP	MF_01168. AGOG. [Tree]
InterPro	IPR015254. N-Glyclase/DNA_lyase-like_arc. IPR016544. N-Glyclase/DNA_lyase_arc. [Graphical view]
Pfam	PF09171. DUF1886. 1 hit. [Graphical view]
PIRSF	PIRSF008955. AGOG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AGOG_AERPE

Accession

Primary (citable) accession number: Q9YE60

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 26, 2007
Last modified:	January 19, 2010
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)