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Q9YE01

- PGMI_AERPE

UniProt

Q9YE01 - PGMI_AERPE

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Protein

Bifunctional phosphoglucose/phosphomannose isomerase

Gene

APE_0768.1

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency.

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.
D-mannose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Inhibited by erythrose 4-phosphate and 6-phosphogluconate.

Kineticsi

  1. KM=3.5 mM for glucose 6-phosphate1 Publication
  2. KM=0.44 mM for fructose 6-phosphate1 Publication
  3. KM=1.1 mM for mannose 6-phosphate1 Publication

Vmax=225 mmol/min/mg enzyme with glucose 6-phosphate as substrate (at 80 degrees Celsius)1 Publication

Vmax=195 mmol/min/mg enzyme with fructose 6-phosphate as substrate (at 80 degrees Celsius)1 Publication

Vmax=209 mmol/min/mg enzyme with mannose 6-phosphate as substrate (at 80 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.6.1 Publication

Temperature dependencei

Optimum temperature is above 98 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei211 – 2111Proton acceptorBy similarity
Active sitei227 – 2271Proton donorBy similarity
Active sitei322 – 3221Proton acceptorBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glucose-6-phosphate isomerase activity Source: UniProtKB-EC
  3. mannose-6-phosphate isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciAPER272557:GJD6-558-MONOMER.
BRENDAi5.3.1.8. 171.
SABIO-RKQ9YE01.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional phosphoglucose/phosphomannose isomerase
Alternative name(s):
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Phosphoglucose isomerase
Short name:
PGI
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Ordered Locus Names:APE_0768.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
ProteomesiUP000002518: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Bifunctional phosphoglucose/phosphomannose isomerasePRO_0000227544Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi272557.APE_0768.1.

Structurei

3D structure databases

ProteinModelPortaliQ9YE01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 160139SISPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PGI/PMI family.Curated
Contains 1 SIS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0166.
HOGENOMiHOG000222946.
KOiK15916.

Family and domain databases

InterProiIPR011857. Glu6P/Mann6P_isomerase.
IPR019490. Glu6P/Mann6P_isomerase_C.
IPR001347. SIS.
[Graphical view]
PfamiPF10432. bact-PGI_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02128. G6PI_arch. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9YE01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDLYLSWPK WFSEALARYS SLEGALEGVE EIYYCGMGGS GAAGDYIEAL
60 70 80 90 100
LSIYAPQGPE FRVVKDFRPP RPPRHRGYGL VLASYSGNTL ETVECGSLLS
110 120 130 140 150
PAAGRVVAVT SGGRLLEMAK ERGWLVARLP GGILPRVSFP WMLAASTAML
160 170 180 190 200
SGALGVDLEA LKRLAGGLDT QGLKGEAERL AGFISRYRIA SLVTCGPGIP
210 220 230 240 250
LAVRLKNELA ENAKMPSRLE IYPESSHNDI VALEAAEGLY GAVFIWIEHE
260 270 280 290 300
GSLCPAVLDV VEGIYREYGV DTISIESSAR GGPNATVAEY LSRTLVFGLA
310 320 330
SVRLALMRGF NPEETPPIDK YKRRLGEALR SQA
Length:333
Mass (Da):35,942
Last modified:June 26, 2007 - v2
Checksum:i3E94DE8CCF7E5DC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA79746.2.
PIRiB72668.
RefSeqiNP_147479.2. NC_000854.2.

Genome annotation databases

EnsemblBacteriaiBAA79746; BAA79746; APE_0768.1.
GeneIDi1444882.
KEGGiape:APE_0768.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA79746.2 .
PIRi B72668.
RefSeqi NP_147479.2. NC_000854.2.

3D structure databases

ProteinModelPortali Q9YE01.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272557.APE_0768.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA79746 ; BAA79746 ; APE_0768.1 .
GeneIDi 1444882.
KEGGi ape:APE_0768.1.

Phylogenomic databases

eggNOGi COG0166.
HOGENOMi HOG000222946.
KOi K15916.

Enzyme and pathway databases

BioCyci APER272557:GJD6-558-MONOMER.
BRENDAi 5.3.1.8. 171.
SABIO-RK Q9YE01.

Family and domain databases

InterProi IPR011857. Glu6P/Mann6P_isomerase.
IPR019490. Glu6P/Mann6P_isomerase_C.
IPR001347. SIS.
[Graphical view ]
Pfami PF10432. bact-PGI_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02128. G6PI_arch. 1 hit.
PROSITEi PS51464. SIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.
  2. "Bifunctional phosphoglucose/phosphomannose isomerases from the archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily."
    Hansen T., Wendor ff D., Schoenheit P.
    J. Biol. Chem. 279:2262-2272(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiPGMI_AERPE
AccessioniPrimary (citable) accession number: Q9YE01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: June 26, 2007
Last modified: October 1, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3