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Q9YE01

- PGMI_AERPE

UniProt

Q9YE01 - PGMI_AERPE

Protein

Bifunctional phosphoglucose/phosphomannose isomerase

Gene

APE_0768.1

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency.

    Catalytic activityi

    D-glucose 6-phosphate = D-fructose 6-phosphate.
    D-mannose 6-phosphate = D-fructose 6-phosphate.

    Enzyme regulationi

    Inhibited by erythrose 4-phosphate and 6-phosphogluconate.

    Kineticsi

    1. KM=3.5 mM for glucose 6-phosphate1 Publication
    2. KM=0.44 mM for fructose 6-phosphate1 Publication
    3. KM=1.1 mM for mannose 6-phosphate1 Publication

    Vmax=225 mmol/min/mg enzyme with glucose 6-phosphate as substrate (at 80 degrees Celsius)1 Publication

    Vmax=195 mmol/min/mg enzyme with fructose 6-phosphate as substrate (at 80 degrees Celsius)1 Publication

    Vmax=209 mmol/min/mg enzyme with mannose 6-phosphate as substrate (at 80 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.6.1 Publication

    Temperature dependencei

    Optimum temperature is above 98 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei211 – 2111Proton acceptorBy similarity
    Active sitei227 – 2271Proton donorBy similarity
    Active sitei322 – 3221Proton acceptorBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glucose-6-phosphate isomerase activity Source: UniProtKB-EC
    3. mannose-6-phosphate isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciAPER272557:GJD6-558-MONOMER.
    BRENDAi5.3.1.8. 171.
    SABIO-RKQ9YE01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional phosphoglucose/phosphomannose isomerase
    Alternative name(s):
    Glucose-6-phosphate isomerase (EC:5.3.1.9)
    Short name:
    GPI
    Mannose-6-phosphate isomerase (EC:5.3.1.8)
    Phosphoglucose isomerase
    Short name:
    PGI
    Phosphomannose isomerase
    Short name:
    PMI
    Gene namesi
    Ordered Locus Names:APE_0768.1
    OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
    Taxonomic identifieri272557 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
    ProteomesiUP000002518: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 333333Bifunctional phosphoglucose/phosphomannose isomerasePRO_0000227544Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi272557.APE_0768.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9YE01.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 160139SISPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PGI/PMI family.Curated
    Contains 1 SIS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0166.
    HOGENOMiHOG000222946.
    KOiK15916.

    Family and domain databases

    InterProiIPR011857. Glu6P/Mann6P_isomerase.
    IPR019490. Glu6P/Mann6P_isomerase_C.
    IPR001347. SIS.
    [Graphical view]
    PfamiPF10432. bact-PGI_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02128. G6PI_arch. 1 hit.
    PROSITEiPS51464. SIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9YE01-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDLYLSWPK WFSEALARYS SLEGALEGVE EIYYCGMGGS GAAGDYIEAL    50
    LSIYAPQGPE FRVVKDFRPP RPPRHRGYGL VLASYSGNTL ETVECGSLLS 100
    PAAGRVVAVT SGGRLLEMAK ERGWLVARLP GGILPRVSFP WMLAASTAML 150
    SGALGVDLEA LKRLAGGLDT QGLKGEAERL AGFISRYRIA SLVTCGPGIP 200
    LAVRLKNELA ENAKMPSRLE IYPESSHNDI VALEAAEGLY GAVFIWIEHE 250
    GSLCPAVLDV VEGIYREYGV DTISIESSAR GGPNATVAEY LSRTLVFGLA 300
    SVRLALMRGF NPEETPPIDK YKRRLGEALR SQA 333
    Length:333
    Mass (Da):35,942
    Last modified:June 26, 2007 - v2
    Checksum:i3E94DE8CCF7E5DC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79746.2.
    PIRiB72668.
    RefSeqiNP_147479.2. NC_000854.2.

    Genome annotation databases

    EnsemblBacteriaiBAA79746; BAA79746; APE_0768.1.
    GeneIDi1444882.
    KEGGiape:APE_0768.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA79746.2 .
    PIRi B72668.
    RefSeqi NP_147479.2. NC_000854.2.

    3D structure databases

    ProteinModelPortali Q9YE01.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272557.APE_0768.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA79746 ; BAA79746 ; APE_0768.1 .
    GeneIDi 1444882.
    KEGGi ape:APE_0768.1.

    Phylogenomic databases

    eggNOGi COG0166.
    HOGENOMi HOG000222946.
    KOi K15916.

    Enzyme and pathway databases

    BioCyci APER272557:GJD6-558-MONOMER.
    BRENDAi 5.3.1.8. 171.
    SABIO-RK Q9YE01.

    Family and domain databases

    InterProi IPR011857. Glu6P/Mann6P_isomerase.
    IPR019490. Glu6P/Mann6P_isomerase_C.
    IPR001347. SIS.
    [Graphical view ]
    Pfami PF10432. bact-PGI_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02128. G6PI_arch. 1 hit.
    PROSITEi PS51464. SIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.
    2. "Bifunctional phosphoglucose/phosphomannose isomerases from the archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily."
      Hansen T., Wendor ff D., Schoenheit P.
      J. Biol. Chem. 279:2262-2272(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

    Entry informationi

    Entry nameiPGMI_AERPE
    AccessioniPrimary (citable) accession number: Q9YE01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3