Q9YDY7 (TRM1_AERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(26)-N(2))-dimethyltransferase
EC=2.1.1.216

Alternative name(s):
tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA(m(2,2)G26)dimethyltransferase

Gene names

Name:	trm1
Ordered Locus Names:	APE_0782.1

Organism

Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) [Complete proteome] [HAMAP]

Taxonomic identifier

272557 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups By similarity. HAMAP MF_00290
Catalytic activity	2 S-adenosyl-L-methionine + guanine(26) in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26) in tRNA. HAMAP MF_00290
Sequence similarities	Belongs to the TRM1 family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	tRNA (guanine(26)-N(2))-dimethyltransferase HAMAP MF_00290		PRO_0000147679

Sequences

	Sequence		Length	Mass (Da)
	Q9YDY7 [UniParc]. Last modified June 26, 2007. Version 3. Checksum: 68E8EFFDF7AE3087	FASTA	407	43,643
	10 20 30 40 50 60 MPVPSPMRLA VTHEGRSIIY IPSPEYAVAS GRLEPAWLEV FYNPAMEFNR DVSVVAASAL 70 80 90 100 110 120 RRTGLLTRSG VAFDAHAGVG VRGVRYAVEA GYVKVIMNDI NPKASMLAAL NARANGLEPG 130 140 150 160 170 180 SYMIFNKESN SLMFHLSRER PTPVSLIDID PYGSPAPFVD AALALSGKGT VVAMTATDLA 190 200 210 220 230 240 VLEGGKARAA VRRYMLRSVS KTPVSKETGL RVLLGYVARV AAAHDKAVKP LLAYYADHYY 250 260 270 280 290 300 RVYVAVERGA RRSDSMLEEN LGRLVYCPET GVALALSYAE DPASACGGSY VVAADPAWIG 310 320 330 340 350 360 SLGDQAFLEA MLNIAVEAVW LGTRPRVEKL LNTLHGEAPL SPRSIYVSLT SVASKARVNT 370 380 390 400 PKKSKVVELL RSMGYRAVAT HFSGEGVRTD APWGEVLEAV VKLGSSG

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000002 Genomic DNA. Translation: BAA79760.2.
PIR	H72669.
RefSeq	NP_147490.2. NC_000854.2.
3D structure databases
ProteinModelPortal	Q9YDY7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1444893.
GenomeReviews	Gene locus APE_0782.1 in contig BA000002_GR.
KEGG	ape:APE_0782.1.
NMPDR	fig|272557.1.peg.598.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG577872.
ProtClustDB	PRK04338.
Enzyme and pathway databases
BioCyc	APER272557:APE0782-MONOMER.
Family and domain databases
HAMAP	MF_00290. tRNA_dimethyltr_TRM1. [Tree]
InterPro	IPR022923. DimeGuo_tRNA_meTrfase_arc. IPR002905. TRM_MeTrfase. [Graphical view]
KO	K00555.
PANTHER	PTHR10631. TRM_mtfrase. 1 hit.
Pfam	PF02005. TRM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00308. TRM1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM1_AERPE

Accession

Primary (citable) accession number: Q9YDY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	June 26, 2007
Last modified:	January 25, 2012
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program