Reviewed, UniProtKB/Swiss-Prot Q9YDW0 (SYT1_AERPE)

Names and origin

Protein names

Recommended name:
    Probable threonyl-tRNA synthetase 1
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase 1
      Short name=ThrRS 1

Gene names

Name:	thrS1
Ordered Locus Names:	APE_0809.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Miscellaneous	There are two ThrRS in this archaea. The first one (APE_0809.1) is most similar to bacterial ThrRS but it lack the N-terminal domain. The second one (APE_0117.1) is most similar to archaeal ThrRS but lack the central domain. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Probable threonyl-tRNA synthetase 1 HAMAP MF_00184		PRO_0000101117
Regions
	Region	20 – 333	314	Catalytic HAMAP MF_00184
Sites
	Metal binding	112	1	Zinc; catalytic By similarity
	Metal binding	166	1	Zinc; catalytic By similarity
	Metal binding	310	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YDW0-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 8AA642538F4DDD7A	FASTA	471	53,122
	10 20 30 40 50 60 MASGQDKTHI DYAYELDITV KPDSRVPVFN REFATFTGAG VPLFSLGGGP IRYALAEVLA 70 80 90 100 110 120 KFHARRGYYV VETPIIASTE LFKVSGHIEF YRNNMYLFDI EGHEFAVKPM NCPYHILLFL 130 140 150 160 170 180 NEVAKHRSKL PLPFKVFEFG RVHRYEPSGS IYGLLRVRGF TQDDAHIIVP GGRVIDVVYD 190 200 210 220 230 240 VFEEMKLVLE RLFKLGVSSE TFKVRLSMSD KSLIGKEFMG SKEEWEGAEE ALREAASRIN 250 260 270 280 290 300 EKYGIDIVEL EGEAAFYGPK LDFIMMVEES GVSKEWQMGT IQFDFNLPRR FRLYDVVREE 310 320 330 340 350 360 FGIEEVYIIH RALLGSIERF LGVYLEHRRG RMPFTLAPIQ FAVIAVKTGG EVDREIEDLA 370 380 390 400 410 420 SSIAKGLLDK GFRVAVKGSS KTGLSSDVRH IESTAKPAVN VFIGAKEVRE KVLDVRVFDL 430 440 450 460 470 ESMKRRRLAI AYGDAADAVE NLAAVAEELE SPVRSLSGQA PRIPADFSFM L

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA79787.2.
PIR	C72673.
RefSeq	NP_147510.2.
3D structure databases
HSSP	HSSP built from PDB template 1EVL based on UniProtKB P00955.
ModBase	Search...
Genome annotation databases
GeneID	1444913.
GenomeReviews	Gene locus APE_0809.1 in contig BA000002_GR.
KEGG	ape:APE_0809.1.
NMPDR	fig|272557.1.peg.618.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BRENDA	6.1.1.3. 256344.
Family and domain databases
HAMAP	MF_00184. Divergent sequence. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR018158. Thr-tRNA-synth_IIa_cons-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT1_AERPE

Accession

Primary (citable) accession number: Q9YDW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 26, 2007
Last modified:	July 28, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)