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Protein

DNA ligase

Gene

lig

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Can also use ADP, but not NAD+.1 Publication

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation1 Publication
ADP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + phosphate.1 Publication
GTP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + GMP + diphosphate.By similarity

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Less active with Ca2+ or Co2+. Not active with Cu2+, Zn2+, Sr2+ or Ni2+.1 Publication

Enzyme regulationi

Inhibited in the presence of 100 mM KCl, NaCl or NH4Cl.1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Temperature dependencei

Optimum temperature is 70 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei262ATPUniRule annotation1
Active sitei264N6-AMP-lysine intermediateUniRule annotation1
Binding sitei269ATPUniRule annotation1
Binding sitei284ATPUniRule annotation1
Binding sitei314ATPUniRule annotation1
Binding sitei354ATPUniRule annotation1
Binding sitei431ATPUniRule annotation1
Binding sitei437ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.5.1.1 171

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase1 PublicationUniRule annotation (EC:6.5.1.71 Publication)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP/ADP]Curated
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:APE_1094.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi
  • UP000002518 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000596001 – 602DNA ligaseAdd BLAST602

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi272557.APE_1094.1

Structurei

3D structure databases

ProteinModelPortaliQ9YD18
SMRiQ9YD18
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiarCOG01347 Archaea
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWIKYKRD
OrthoDBiPOG093Z03L0

Family and domain databases

Gene3Di1.10.3260.101 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9YD18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFKPVAEAF ASMERITSRT QLTLLLTRLF KSTPPGAIGI VVYLIQGKLG
60 70 80 90 100
PDWKGLPELG VGEKLLVKAI ALAYKATEER VERLYKSVGD LGSVAERLSR
110 120 130 140 150
EYRSRAARAV TLEAFMAGGG EALTVRRVYN TLYRIAMAQG EGSRDIKLRL
160 170 180 190 200
LAGLLADAEP VEAKYIVRFV EGRLRVGVGD ATVLDALAMA FGGGAHARPV
210 220 230 240 250
IERAYNLRAD LGYIAEVVAR EGVDALRGVK PQVGVPIRPM LAERGRDPAE
260 270 280 290 300
ILRKVGGRAV VEYKYDGERA QIHKKDGEVY IYSRRLENIT RMFPDVVEMA
310 320 330 340 350
RKGLKAGEAI VEGEIVAVDP DNYEIQPFQV LMQRKRKHDI HRVMREVPVA
360 370 380 390 400
VFLFDALYVD GEDLTSKPLP ERRRRLKEIV VETPLWRLAE SIETSDPEEL
410 420 430 440 450
WTFFLKAIEE GAEGVMVKAV HRDSVYTAGV RGWLWVKLKR DYKSEMMDTV
460 470 480 490 500
DLVVVGAFYG RGKRGGKLSS LLMAAYDPDR DVFPTVCKVA TGFTDEELDR
510 520 530 540 550
MNEMLKKHII PRKHPRVESR IEPDVWVEPA LVAEILGAEL TLSPMHTCCL
560 570 580 590 600
NTVRPGVGIS IRFPRFIRWR DDKSPEDATT THELLEMYKR QLRRVEEPAE

QV
Length:602
Mass (Da):67,748
Last modified:June 20, 2001 - v2
Checksum:iAD1DC7521FAFD814
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA Translation: BAA80079.2
PIRiG72709

Genome annotation databases

EnsemblBacteriaiBAA80079; BAA80079; APE_1094.1
KEGGiape:APE_1094.1
PATRICifig|272557.25.peg.768

Similar proteinsi

Entry informationi

Entry nameiDNLI_AERPE
AccessioniPrimary (citable) accession number: Q9YD18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: March 28, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome