Reviewed, UniProtKB/Swiss-Prot Q9YCY3 (SYM_AERPE)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	APE_1129

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00098
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139182
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00098
	Motif	334 – 338	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	337	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YCY3-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 6E2101CEFB666E4E	FASTA	572	66,481
	10 20 30 40 50 60 MAKYVVTSAW PYVNHVPHLG TLIGSVLSAD IYARYLRLRG RQVVFVSGSD EHGTPIELEA 70 80 90 100 110 120 RKKGVHPKEL TDQVHEYDVK MWREYRISFD NYSRTESPVH KEFVMEFMKK LEENGYIFSQ 130 140 150 160 170 180 EEVLPYCERD KIFLPDRFVE GTCPYCGYEK ARGDQCDECG RLLHPTELKN PRCALCGSKP 190 200 210 220 230 240 VYKSTRHWFI DLRRVQDRLL KWLESHGELQ DSVKKYSINW VAQGLKPRSV TRDLSWGVPA 250 260 270 280 290 300 PFKGAEGKTI YVWFDALLGY VSATKELFIM RRGDPEEWKS WWWDSGTRTV YFIGKDNIPF 310 320 330 340 350 360 HAIILPALFL ASHDPYVLPW RISATEYLMY EGQQFSKSRR IGVWIDEALE IAPADYWRWA 370 380 390 400 410 420 LARMRPEARD TNFTWKEFYR IVNTELNDDI GNFVNRVLSL VRSRMSGVAP EPEDLGEHQG 430 440 450 460 470 480 FVERVRRAAW AVAEDLEAIR IKRATEGILE IAREGNAYLN RTQPWKLLSQ DREEGVRALS 490 500 510 520 530 540 AALYAVKTLA HLLAPFTPDA ADRLWSMLGL AGSVHEAVWD EWLDRPLPGG ARIVRVEPLF 550 560 570 QKLPDDFLER VDEIVEDARR RAREKRPPLL RD

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA80114.1.
PIR	B72714.
RefSeq	NP_147731.1.
3D structure databases
HSSP	HSSP built from PDB template 1MEA based on UniProtKB P00959.
ModBase	Search...
Genome annotation databases
GeneID	1445805.
GenomeReviews	Gene locus APE_1129 in contig BA000002_GR.
KEGG	ape:APE_1129.
NMPDR	fig|272557.1.peg.839.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YCY3.
OMA	FTEEWAA.
Enzyme and pathway databases
BRENDA	6.1.1.10. 256344.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_AERPE

Accession

Primary (citable) accession number: Q9YCY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)