Reviewed, UniProtKB/Swiss-Prot Q9YC88 (RISB_AERPE)

Names and origin

Protein names

Recommended name:
    6,7-dimethyl-8-ribityllumazine synthase
      Short name=DMRL synthase
      Short name=Lumazine synthase
    EC=2.5.1.9
Alternative name(s):
    Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	APE_1366.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_0000134839

Sequences

	Sequence		Length	Mass (Da)
	Q9YC88-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 88E0C555C11209E0	FASTA	147	15,933
	10 20 30 40 50 60 MSCGDKIRIA IVVSEFNYDV TRVMEEKAID HARFLGAEVS LVARSPGTFD TPFIVSRLLA 70 80 90 100 110 120 THSEVDAVAV LGAVIKGDTK HDEVVAHQAA RKLLDLSIEY GKPVTLGIIG PGASRLEAIE 130 140 RAEEYARRAV ESAVKLARRS KELSGEC

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000002 Genomic DNA. Translation: BAA80360.2.
PIR	B72613.
RefSeq	NP_147899.2.
3D structure databases
SMR	Q9YC88. Positions 3-143.
ModBase	Search...
Genome annotation databases
GeneID	1445969.
GenomeReviews	Gene locus APE_1366.1 in contig BA000002_GR.
KEGG	ape:APE_1366.1.
NMPDR	fig|272557.1.peg.1007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311126.
Enzyme and pathway databases
BioCyc	APER272557:APE1366-MONOMER.
BRENDA	2.5.1.9. 256344.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00114. lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_AERPE

Accession

Primary (citable) accession number: Q9YC88

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 26, 2007
Last modified:	February 9, 2010
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)