Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acylamino-acid-releasing enzyme

Gene

APE_1547.1

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus.

Catalytic activityi

Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei445Charge relay systemBy similarity1
Active sitei524Charge relay systemBy similarity1
Active sitei556Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.1.1. 171.
3.4.19.1. 171.

Protein family/group databases

ESTHERiaerpe-APE1547. ACPH_Peptidase_S9.
MEROPSiS09.070.

Names & Taxonomyi

Protein namesi
Recommended name:
Acylamino-acid-releasing enzyme (EC:3.4.19.1)
Short name:
AARE
Alternative name(s):
Acyl-peptide hydrolase
Short name:
APH
Acylaminoacyl-peptidase
Gene namesi
Ordered Locus Names:APE_1547.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi
  • UP000002518 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224361 – 582Acylamino-acid-releasing enzymeAdd BLAST582

Proteomic databases

PRIDEiQ9YBQ2.

Interactioni

Protein-protein interaction databases

STRINGi272557.APE_1547.1.

Structurei

Secondary structure

1582
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 21Combined sources13
Beta strandi24 – 31Combined sources8
Turni32 – 34Combined sources3
Beta strandi35 – 42Combined sources8
Beta strandi45 – 52Combined sources8
Beta strandi55 – 58Combined sources4
Beta strandi65 – 67Combined sources3
Beta strandi75 – 82Combined sources8
Beta strandi90 – 96Combined sources7
Beta strandi103 – 105Combined sources3
Beta strandi111 – 119Combined sources9
Beta strandi124 – 129Combined sources6
Beta strandi134 – 140Combined sources7
Beta strandi143 – 152Combined sources10
Beta strandi154 – 160Combined sources7
Beta strandi163 – 171Combined sources9
Beta strandi174 – 181Combined sources8
Turni182 – 184Combined sources3
Beta strandi188 – 190Combined sources3
Beta strandi196 – 202Combined sources7
Beta strandi208 – 213Combined sources6
Beta strandi218 – 223Combined sources6
Turni225 – 227Combined sources3
Beta strandi230 – 232Combined sources3
Beta strandi236 – 238Combined sources3
Helixi239 – 243Combined sources5
Beta strandi246 – 253Combined sources8
Beta strandi255 – 257Combined sources3
Beta strandi259 – 265Combined sources7
Beta strandi268 – 273Combined sources6
Beta strandi276 – 278Combined sources3
Beta strandi282 – 291Combined sources10
Beta strandi294 – 301Combined sources8
Beta strandi304 – 311Combined sources8
Turni312 – 314Combined sources3
Beta strandi316 – 318Combined sources3
Helixi324 – 328Combined sources5
Beta strandi330 – 339Combined sources10
Beta strandi345 – 353Combined sources9
Beta strandi358 – 366Combined sources9
Beta strandi368 – 371Combined sources4
Helixi380 – 387Combined sources8
Beta strandi391 – 395Combined sources5
Beta strandi401 – 403Combined sources3
Helixi405 – 409Combined sources5
Turni410 – 413Combined sources4
Turni415 – 417Combined sources3
Helixi418 – 432Combined sources15
Beta strandi436 – 444Combined sources9
Helixi446 – 457Combined sources12
Beta strandi461 – 463Combined sources3
Beta strandi465 – 469Combined sources5
Helixi474 – 479Combined sources6
Helixi483 – 492Combined sources10
Turni493 – 495Combined sources3
Helixi497 – 502Combined sources6
Helixi505 – 511Combined sources7
Beta strandi516 – 521Combined sources6
Beta strandi525 – 527Combined sources3
Helixi529 – 541Combined sources13
Beta strandi546 – 551Combined sources6
Helixi561 – 579Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VE6X-ray2.10A/B1-582[»]
1VE7X-ray2.70A/B1-582[»]
2HU5X-ray2.00A/B1-582[»]
2HU7X-ray2.01A/B1-582[»]
2HU8X-ray2.40A/B1-582[»]
2QR5X-ray2.20A/B1-582[»]
2QZPX-ray2.70A/B21-582[»]
3O4GX-ray2.50A/B/C/D1-582[»]
3O4HX-ray1.82A/B/C/D1-582[»]
3O4IX-ray2.70A/B1-582[»]
3O4JX-ray2.50A/B/C/D1-582[»]
4RE5X-ray1.90A/B1-582[»]
4RE6X-ray2.55A/B/C/D1-582[»]
DisProtiDP00248.
ProteinModelPortaliQ9YBQ2.
SMRiQ9YBQ2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9YBQ2.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9C family.Curated

Phylogenomic databases

eggNOGiarCOG01646. Archaea.
COG1506. LUCA.
HOGENOMiHOG000115412.
KOiK01303.
OMAiDIGDPGG.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
3.40.50.1820. 2 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
[Graphical view]
PfamiPF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9YBQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIIMPVEFS RIVRDVERLI AVEKYSLQGV VDGDKLLVVG FSEGSVNAYL
60 70 80 90 100
YDGGETVKLN REPINSVLDP HYGVGRVILV RDVSKGAEQH ALFKVNTSRP
110 120 130 140 150
GEEQRLEAVK PMRILSGVDT GEAVVFTGAT EDRVALYALD GGGLRELARL
160 170 180 190 200
PGFGFVSDIR GDLIAGLGFF GGGRVSLFTS NLSSGGLRVF DSGEGSFSSA
210 220 230 240 250
SISPGMKVTA GLETAREARL VTVDPRDGSV EDLELPSKDF SSYRPTAITW
260 270 280 290 300
LGYLPDGRLA VVARREGRSA VFIDGERVEA PQGNHGRVVL WRGKLVTSHT
310 320 330 340 350
SLSTPPRIVS LPSGEPLLEG GLPEDLRRSI AGSRLVWVES FDGSRVPTYV
360 370 380 390 400
LESGRAPTPG PTVVLVHGGP FAEDSDSWDT FAASLAAAGF HVVMPNYRGS
410 420 430 440 450
TGYGEEWRLK IIGDPCGGEL EDVSAAARWA RESGLASELY IMGYSYGGYM
460 470 480 490 500
TLCALTMKPG LFKAGVAGAS VVDWEEMYEL SDAAFRNFIE QLTGGSREIM
510 520 530 540 550
RSRSPINHVD RIKEPLALIH PQNDSRTPLK PLLRLMGELL ARGKTFEAHI
560 570 580
IPDAGHAINT MEDAVKILLP AVFFLATQRE RR
Length:582
Mass (Da):63,035
Last modified:November 1, 1999 - v1
Checksum:iA9F2DD39ACD1C3DF
GO

Sequence cautioni

The sequence BAA80546 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA80546.2. Different initiation.
PIRiD72636.

Genome annotation databases

EnsemblBacteriaiBAA80546; BAA80546; APE_1547.1.
KEGGiape:APE_1547.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA80546.2. Different initiation.
PIRiD72636.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VE6X-ray2.10A/B1-582[»]
1VE7X-ray2.70A/B1-582[»]
2HU5X-ray2.00A/B1-582[»]
2HU7X-ray2.01A/B1-582[»]
2HU8X-ray2.40A/B1-582[»]
2QR5X-ray2.20A/B1-582[»]
2QZPX-ray2.70A/B21-582[»]
3O4GX-ray2.50A/B/C/D1-582[»]
3O4HX-ray1.82A/B/C/D1-582[»]
3O4IX-ray2.70A/B1-582[»]
3O4JX-ray2.50A/B/C/D1-582[»]
4RE5X-ray1.90A/B1-582[»]
4RE6X-ray2.55A/B/C/D1-582[»]
DisProtiDP00248.
ProteinModelPortaliQ9YBQ2.
SMRiQ9YBQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272557.APE_1547.1.

Protein family/group databases

ESTHERiaerpe-APE1547. ACPH_Peptidase_S9.
MEROPSiS09.070.

Proteomic databases

PRIDEiQ9YBQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA80546; BAA80546; APE_1547.1.
KEGGiape:APE_1547.1.

Phylogenomic databases

eggNOGiarCOG01646. Archaea.
COG1506. LUCA.
HOGENOMiHOG000115412.
KOiK01303.
OMAiDIGDPGG.

Enzyme and pathway databases

BRENDAi3.1.1.1. 171.
3.4.19.1. 171.

Miscellaneous databases

EvolutionaryTraceiQ9YBQ2.

Family and domain databases

Gene3Di2.120.10.30. 2 hits.
3.40.50.1820. 2 hits.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
[Graphical view]
PfamiPF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPEH_AERPE
AccessioniPrimary (citable) accession number: Q9YBQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.