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Q9YBQ2 (APEH_AERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Acylamino-acid-releasing enzyme
Short name=AARE
EC=3.4.19.1
Alternative name(s):
Acyl-peptide hydrolase
Short name=APH
Acylaminoacyl-peptidase
Gene names
Ordered Locus Names:APE_1547.1
OrganismAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) [Complete proteome] [HAMAP]
Taxonomic identifier272557 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus.

Catalytic activity

Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the peptidase S9C family.

Sequence caution

The sequence BAA80546.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 582582Acylamino-acid-releasing enzyme
PRO_0000122436

Sites

Active site4451Charge relay system By similarity
Active site5241Charge relay system By similarity
Active site5561Charge relay system By similarity

Secondary structure

1 ............................................................................................................ 582
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9YBQ2 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: A9F2DD39ACD1C3DF

FASTA58263,035
        10         20         30         40         50         60 
MRIIMPVEFS RIVRDVERLI AVEKYSLQGV VDGDKLLVVG FSEGSVNAYL YDGGETVKLN 

        70         80         90        100        110        120 
REPINSVLDP HYGVGRVILV RDVSKGAEQH ALFKVNTSRP GEEQRLEAVK PMRILSGVDT 

       130        140        150        160        170        180 
GEAVVFTGAT EDRVALYALD GGGLRELARL PGFGFVSDIR GDLIAGLGFF GGGRVSLFTS 

       190        200        210        220        230        240 
NLSSGGLRVF DSGEGSFSSA SISPGMKVTA GLETAREARL VTVDPRDGSV EDLELPSKDF 

       250        260        270        280        290        300 
SSYRPTAITW LGYLPDGRLA VVARREGRSA VFIDGERVEA PQGNHGRVVL WRGKLVTSHT 

       310        320        330        340        350        360 
SLSTPPRIVS LPSGEPLLEG GLPEDLRRSI AGSRLVWVES FDGSRVPTYV LESGRAPTPG 

       370        380        390        400        410        420 
PTVVLVHGGP FAEDSDSWDT FAASLAAAGF HVVMPNYRGS TGYGEEWRLK IIGDPCGGEL 

       430        440        450        460        470        480 
EDVSAAARWA RESGLASELY IMGYSYGGYM TLCALTMKPG LFKAGVAGAS VVDWEEMYEL 

       490        500        510        520        530        540 
SDAAFRNFIE QLTGGSREIM RSRSPINHVD RIKEPLALIH PQNDSRTPLK PLLRLMGELL 

       550        560        570        580 
ARGKTFEAHI IPDAGHAINT MEDAVKILLP AVFFLATQRE RR

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1."
Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. expand/collapse author list , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.
DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.
[2]"Crystallization and preliminary crystallographic analysis of acylamino-acid releasing enzyme from the hyperthermophilic archaeon Aeropyrum pernix."
Wang G., Gao R., Ding Y., Yang H., Cao S., Feng Y., Rao Z.
Acta Crystallogr. D 58:1054-1055(2002) [PubMed: 12037315] [Abstract]
Cited for: CRYSTALLIZATION.
Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000002 Genomic DNA. Translation: BAA80546.2. Different initiation.
PIRD72636.
RefSeqNP_148014.2. NC_000854.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VE6X-ray2.10A/B1-582[»]
1VE7X-ray2.70A/B1-582[»]
2HU5X-ray2.00A/B1-582[»]
2HU7X-ray2.01A/B1-582[»]
2HU8X-ray2.40A/B1-582[»]
2QR5X-ray2.20A/B1-582[»]
2QZPX-ray2.70A/B21-582[»]
3O4GX-ray2.50A/B/C/D1-582[»]
3O4HX-ray1.82A/B/C/D1-582[»]
3O4IX-ray2.70A/B1-582[»]
3O4JX-ray2.50A/B/C/D1-582[»]
ProteinModelPortalQ9YBQ2.
SMRQ9YBQ2. Positions 8-582.
DisProtDP00248.
ModBaseSearch...

Protein family/group databases

MEROPSS09.070.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1446086.
GenomeReviewsGene locus APE_1547.1 in contig BA000002_GR.
KEGGape:APE_1547.1.
NMPDRfig|272557.1.peg.1122.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG297261.
OMAGESYWIY.
PhylomeDBQ9YBQ2.
ProtClustDBCLSK270850.

Enzyme and pathway databases

BioCycAPER272557:APE1547-MONOMER.

Family and domain databases

InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR001375. Peptidase_S9.
IPR004106. Peptidase_S9A_B_C_N.
[Graphical view]
Gene3DG3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
KOK01303.
PfamPF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMSSF50993. Peptidase_S9A_N. 1 hit.
PROSITEPS00708. PRO_ENDOPEP_SER. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPEH_AERPE
AccessionPrimary (citable) accession number: Q9YBQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 1, 1999
Last modified: December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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