Reviewed, UniProtKB/Swiss-Prot Q9YBK6 (SYC_AERPE)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	APE_1592.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_0000159532
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00041
	Motif	264 – 268	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	207	1	Zinc By similarity
	Metal binding	232	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Binding site	267	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YBK6-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 3B911DA0510F1A1A	FASTA	472	55,421
	10 20 30 40 50 60 MIRVFNTLGR RKEVFNPYSP PLVGMYVCGP TVYDYTHIGH ARTFTVFDAI KRYLRLRGYD 70 80 90 100 110 120 VFHVQNITDI DDKIINRARE EGRDWREIVR EYSRDYLEGL GSLGIQIDHH PRVTDHISDI 130 140 150 160 170 180 IRFIEGLIEK GYAYVAESGS VYFEVDKYPG YGMLSGHLSK EAWRQEEDVL HEKKSPYDFA 190 200 210 220 230 240 LWKAAKPGEP SWESPWGRGR PGWHIECSVM SSRYLGSRID IHGGGVDLVF PHHENERAQS 250 260 270 280 290 300 ESYFGHRWVR YWLHASYLTI KGEKMSKSLG NIIPLREALR EWGPGPLRLW LLSSHYRSNL 310 320 330 340 350 360 DYSEEALGQY RRLYERLRQA ADSIGRRLER AEPKGRLGEG ELETVERLKG VVRRWHEAMS 370 380 390 400 410 420 DDFNMGKAMS SLWEFTTIYF REVEQTESYT LLWLSWRILT GFNSVYAFAP DIVEARRPER 430 440 450 460 470 SLEDSLVQAL VDVRSELRRR KMYDLADEIR SRLAQLGFVL HDKGEKTEWR RR

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA80592.2.
PIR	C72538.
RefSeq	NP_148045.2.
3D structure databases
HSSP	HSSP built from PDB template 1LI7 based on UniProtKB P21888.
ModBase	Search...
Genome annotation databases
GeneID	1446118.
GenomeReviews	Gene locus APE_1592.1 in contig BA000002_GR.
KEGG	ape:APE_1592.1.
NMPDR	fig|272557.1.peg.1153.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YBK6.
Enzyme and pathway databases
BRENDA	6.1.1.16. 256344.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. [Graphical view]
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_AERPE

Accession

Primary (citable) accession number: Q9YBK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 26, 2007
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)