Reviewed, UniProtKB/Swiss-Prot Q9YBA5 (IPYR_AERPE)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	APE_1692.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137547
Sites
	Metal binding	66	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 2 By similarity
	Metal binding	103	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YBA5-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: FCB753D5E972C532	FASTA	176	19,527
	10 20 30 40 50 60 MTGCLKIGPG DEAPDVVNVV IEIPMNSSVK YEFDKEACIV KVDRFLYTSM VYPFNYGFIP 70 80 90 100 110 120 GTLEEDGDPV DVLVISREPV APGSLIEAVP VAVLDMEDEE GPDSKVVAVP KAKLDPLFAS 130 140 150 160 170 YKDVGDIPDA LKSKIKHFFE HYKELEPGKW VRVTGWRPAA DAKEIIRRAI ERYKGA

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA80693.2.
PIR	H72550.
RefSeq	NP_148101.2.
3D structure databases
HSSP	HSSP built from PDB template 1QEZ based on UniProtKB P50308.
ModBase	Search...
Genome annotation databases
GeneID	1446175.
GenomeReviews	Gene locus APE_1692.1 in contig BA000002_GR.
KEGG	ape:APE_1692.1.
NMPDR	fig|272557.1.peg.1209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YBA5.
OMA	YRDEIEN.
Enzyme and pathway databases
BRENDA	3.6.1.1. 256344.
Family and domain databases
HAMAP	MF_00209. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_AERPE

Accession

Primary (citable) accession number: Q9YBA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 3, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)