Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD-dependent protein deacylase

Gene

cobB

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.UniRule annotation

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.UniRule annotation
NAD+ + a succinylprotein = nicotinamide + O-succinyl-ADP-ribose + a protein.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661SubstrateUniRule annotation
Binding sitei69 – 691SubstrateUniRule annotation
Active sitei118 – 1181Proton acceptorUniRule annotation
Metal bindingi126 – 1261ZincUniRule annotation
Metal bindingi129 – 1291ZincUniRule annotation
Metal bindingi146 – 1461ZincUniRule annotation
Metal bindingi149 – 1491ZincUniRule annotation
Binding sitei241 – 2411NAD; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 4120NADUniRule annotationAdd
BLAST
Nucleotide bindingi100 – 1034NADUniRule annotation
Nucleotide bindingi186 – 1883NADUniRule annotation
Nucleotide bindingi212 – 2143NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacylaseUniRule annotation (EC:3.5.1.-UniRule annotation)
Alternative name(s):
Regulatory protein SIR2 homologUniRule annotation
Gene namesi
Name:cobBUniRule annotation
Ordered Locus Names:APE_1782.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi
  • UP000002518 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245NAD-dependent protein deacylasePRO_0000110376Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272557.APE_1782.1.

Structurei

3D structure databases

ProteinModelPortaliQ9YB13.
SMRiQ9YB13. Positions 6-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 245241Deacetylase sirtuin-typeUniRule annotationAdd
BLAST

Domaini

2 residues (Tyr-66 and Arg-69) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the sirtuin family. Class III subfamily.UniRule annotation
Contains 1 deacetylase sirtuin-type domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04248. Archaea.
COG0846. LUCA.
HOGENOMiHOG000085950.
KOiK12410.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01121. Sirtuin_ClassIII.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_class_III.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9YB13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVWESARI LANSRFAVAF TGAGISAESG IPTFRGKDGL WSRFDPRDLA
60 70 80 90 100
TPEAFNRDPR LVWEWYSWRI ERVLAAKPNK AHRLLARLED SGVLKAVITQ
110 120 130 140 150
NVDGLHRRAG SRRVLELHGN VLRARCTRCG SKLEWREKPS NLPPSCPRCG
160 170 180 190 200
GVLRPDVVWF GEPLDTSLLE EAFGLARRSD VMIIIGTSGA VDPAGLLPLA
210 220 230 240
AKESGATLIN VNPEPNRYSG VADIELRMRA VEFAERLSRA MGIDI
Length:245
Mass (Da):27,096
Last modified:July 10, 2007 - v2
Checksum:iED1DEDCE38BDEFA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA80785.2.
PIRiD72562.

Genome annotation databases

EnsemblBacteriaiBAA80785; BAA80785; APE_1782.1.
KEGGiape:APE_1782.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA80785.2.
PIRiD72562.

3D structure databases

ProteinModelPortaliQ9YB13.
SMRiQ9YB13. Positions 6-241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272557.APE_1782.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA80785; BAA80785; APE_1782.1.
KEGGiape:APE_1782.1.

Phylogenomic databases

eggNOGiarCOG04248. Archaea.
COG0846. LUCA.
HOGENOMiHOG000085950.
KOiK12410.

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1201-MONOMER.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01121. Sirtuin_ClassIII.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_class_III.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiNPD_AERPE
AccessioniPrimary (citable) accession number: Q9YB13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: July 10, 2007
Last modified: November 11, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.