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Protein

NAD-dependent protein deacylase

Gene

cobB

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.UniRule annotation

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.UniRule annotation
NAD+ + a succinylprotein = nicotinamide + O-succinyl-ADP-ribose + a protein.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66SubstrateUniRule annotation1
Binding sitei69SubstrateUniRule annotation1
Active sitei118Proton acceptorUniRule annotation1
Metal bindingi126ZincUniRule annotation1
Metal bindingi129ZincUniRule annotation1
Metal bindingi146ZincUniRule annotation1
Metal bindingi149ZincUniRule annotation1
Binding sitei241NAD; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 41NADUniRule annotationAdd BLAST20
Nucleotide bindingi100 – 103NADUniRule annotation4
Nucleotide bindingi186 – 188NADUniRule annotation3
Nucleotide bindingi212 – 214NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTranscription, Transcription regulation
LigandMetal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacylaseUniRule annotation (EC:3.5.1.-UniRule annotation)
Alternative name(s):
Regulatory protein SIR2 homologUniRule annotation
Gene namesi
Name:cobBUniRule annotation
Ordered Locus Names:APE_1782.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001103761 – 245NAD-dependent protein deacylaseAdd BLAST245

Proteomic databases

PRIDEiQ9YB13

Interactioni

Protein-protein interaction databases

STRINGi272557.APE_1782.1

Structurei

3D structure databases

ProteinModelPortaliQ9YB13
SMRiQ9YB13
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 245Deacetylase sirtuin-typeUniRule annotationAdd BLAST241

Domaini

2 residues (Tyr-66 and Arg-69) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the sirtuin family. Class III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04248 Archaea
COG0846 LUCA
HOGENOMiHOG000085950
KOiK12410
OrthoDBiPOG093Z06CT

Family and domain databases

CDDicd01412 SIRT5_Af1_CobB, 1 hit
Gene3Di3.30.1600.10, 2 hits
HAMAPiMF_01121 Sirtuin_ClassIII, 1 hit
InterProiView protein in InterPro
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR003000 Sirtuin
IPR026591 Sirtuin_cat_small_dom_sf
IPR027546 Sirtuin_class_III
IPR026590 Ssirtuin_cat_dom
PfamiView protein in Pfam
PF02146 SIR2, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
PROSITEiView protein in PROSITE
PS50305 SIRTUIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9YB13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVWESARI LANSRFAVAF TGAGISAESG IPTFRGKDGL WSRFDPRDLA
60 70 80 90 100
TPEAFNRDPR LVWEWYSWRI ERVLAAKPNK AHRLLARLED SGVLKAVITQ
110 120 130 140 150
NVDGLHRRAG SRRVLELHGN VLRARCTRCG SKLEWREKPS NLPPSCPRCG
160 170 180 190 200
GVLRPDVVWF GEPLDTSLLE EAFGLARRSD VMIIIGTSGA VDPAGLLPLA
210 220 230 240
AKESGATLIN VNPEPNRYSG VADIELRMRA VEFAERLSRA MGIDI
Length:245
Mass (Da):27,096
Last modified:July 10, 2007 - v2
Checksum:iED1DEDCE38BDEFA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA Translation: BAA80785.2
PIRiD72562

Genome annotation databases

EnsemblBacteriaiBAA80785; BAA80785; APE_1782.1
KEGGiape:APE_1782.1
PATRICifig|272557.25.peg.1194

Entry informationi

Entry nameiNPD_AERPE
AccessioniPrimary (citable) accession number: Q9YB13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: July 10, 2007
Last modified: May 23, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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