Reviewed, UniProtKB/Swiss-Prot Q9YB04 (CCA_AERPE)

Names and origin

Protein names

Recommended name:
    CCA-adding enzyme
    EC=2.7.7.25
    EC=2.7.7.21
Alternative name(s):
    tRNA nucleotidyltransferase
    tRNA adenylyl-/cytidylyl- transferase
    tRNA CCA-pyrophosphorylase
    tRNA-NT

Gene names

Name:	cca
Ordered Locus Names:	APE_1791.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity.
Catalytic activity	ATP + tRNA(n) = diphosphate + tRNA(n+1). HAMAP MF_01264 CTP + tRNA(n) = diphosphate + tRNA(n+1). HAMAP MF_01264
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer By similarity.
Miscellaneous	A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity.
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.

Ontologies

Keywords
Biological process	RNA repair tRNA processing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA repair Inferred from electronic annotation. Source: UniProtKB-KW tRNA 3'-terminal CCA addition Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP tRNA adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	CCA-adding enzyme HAMAP MF_01264		PRO_0000139064
Sites
	Metal binding	75	1	Magnesium By similarity
	Metal binding	77	1	Magnesium By similarity
	Metal binding	127	1	Magnesium By similarity
	Binding site	63	1	ATP or CTP By similarity
	Binding site	66	1	ATP or CTP By similarity
	Binding site	149	1	ATP or CTP By similarity
	Binding site	171	1	ATP or CTP By similarity
	Binding site	180	1	ATP or CTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YB04-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: DF472F68507B2673	FASTA	465	51,778
	10 20 30 40 50 60 MRGASGESVL PEDLRSRVLE RIRPTRLQFS MLGRLYSLVA RALEECEDLR LSIPSYRVEL 70 80 90 100 110 120 VGSAAKGTLL RDKWEVDVFL LLDAPREEVR RLGESLLRSC LGGRLPYYFK YSEHPYATVS 130 140 150 160 170 180 LMGMQADVVP APLAVDPRDA VGVERTPFHT RYVRSRLEER PWLVDDILLF KSFLKGIGVY 190 200 210 220 230 240 GAETRVGGFS GYLAEVLIIH HGGFEETVKA ASSWRPPVMV DTTSGKADLD MLARRYPDSP 250 260 270 280 290 300 IIVPDPVDPS RNTAASVTPK RLAELVHAAN IFLRKPSPTF FHALQPGEPC RRTLPGVMVL 310 320 330 340 350 360 MSGNYSDHPP DSVWGRLKRL GERLYRATKA RGYPALSYSF YTDEALEAAV YIHMESPSRY 370 380 390 400 410 420 PIEGRLGPPP WERERAARFT EKRLGEGGWV WIGDDGRLSG ARPYTGSAAG DVERALATLP 430 440 450 460 LPPGTRSYKV VTCPSTGPCG FPSYLEALRD PTPPWLRHVL GGCRS

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA80794.2.
PIR	E72563.
RefSeq	NP_148168.2.
3D structure databases
HSSP	HSSP built from PDB template 1UET based on UniProtKB O28126.
ModBase	Search...
Genome annotation databases
GeneID	1446242.
GenomeReviews	Gene locus APE_1791.1 in contig BA000002_GR.
KEGG	ape:APE_1791.1.
NMPDR	fig|272557.1.peg.1276.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YB04.
Enzyme and pathway databases
BRENDA	2.7.7.21. 256344. 2.7.7.25. 256344.
Family and domain databases
HAMAP	MF_01264. [Tree]
InterPro	IPR008229. CCA-adding_enz. IPR002934. Nucleotidyltransferase. IPR015329. tRNA_NucTransf2. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. PF09249. tRNA_NucTransf2. 1 hit. [Graphical view]
PIRSF	PIRSF005335. CCA_arch. 1 hit.
TIGRFAMs	TIGR03671. Cca_archaeal. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCA_AERPE

Accession

Primary (citable) accession number: Q9YB04

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 10, 2007
Last modified:	June 16, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)