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Q9YB04 (CCA_AERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
CCA-adding enzyme
EC=2.7.7.72
Alternative name(s):
CCA tRNA nucleotidyltransferase
tRNA CCA-pyrophosphorylase
tRNA adenylyl-/cytidylyl- transferase
tRNA nucleotidyltransferase
tRNA-NT
Gene names
Name:cca
Ordered Locus Names:APE_1791.1
OrganismAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) [Complete proteome] [HAMAP]
Taxonomic identifier272557 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity. HAMAP MF_01264

Catalytic activity

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate. HAMAP MF_01264

Cofactor

Magnesium By similarity. HAMAP MF_01264

Subunit structure

Homodimer By similarity. HAMAP MF_01264

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity. HAMAP MF_01264

Sequence similarities

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465CCA-adding enzyme HAMAP MF_01264
PRO_0000139064

Sites

Metal binding751Magnesium By similarity
Metal binding771Magnesium By similarity
Metal binding1271Magnesium By similarity
Binding site631ATP or CTP By similarity
Binding site661ATP or CTP By similarity
Binding site1491ATP or CTP By similarity
Binding site1711ATP or CTP By similarity
Binding site1801ATP or CTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9YB04 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: DF472F68507B2673

FASTA46551,778
        10         20         30         40         50         60 
MRGASGESVL PEDLRSRVLE RIRPTRLQFS MLGRLYSLVA RALEECEDLR LSIPSYRVEL 

        70         80         90        100        110        120 
VGSAAKGTLL RDKWEVDVFL LLDAPREEVR RLGESLLRSC LGGRLPYYFK YSEHPYATVS 

       130        140        150        160        170        180 
LMGMQADVVP APLAVDPRDA VGVERTPFHT RYVRSRLEER PWLVDDILLF KSFLKGIGVY 

       190        200        210        220        230        240 
GAETRVGGFS GYLAEVLIIH HGGFEETVKA ASSWRPPVMV DTTSGKADLD MLARRYPDSP 

       250        260        270        280        290        300 
IIVPDPVDPS RNTAASVTPK RLAELVHAAN IFLRKPSPTF FHALQPGEPC RRTLPGVMVL 

       310        320        330        340        350        360 
MSGNYSDHPP DSVWGRLKRL GERLYRATKA RGYPALSYSF YTDEALEAAV YIHMESPSRY 

       370        380        390        400        410        420 
PIEGRLGPPP WERERAARFT EKRLGEGGWV WIGDDGRLSG ARPYTGSAAG DVERALATLP 

       430        440        450        460 
LPPGTRSYKV VTCPSTGPCG FPSYLEALRD PTPPWLRHVL GGCRS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000002 Genomic DNA. Translation: BAA80794.2.
PIRE72563.
RefSeqNP_148168.2. NC_000854.2.

3D structure databases

ProteinModelPortalQ9YB04.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1446242.
GenomeReviewsGene locus APE_1791.1 in contig BA000002_GR.
KEGGape:APE_1791.1.
NMPDRfig|272557.1.peg.1276.

Organism-specific databases

CMRSearch...

Phylogenomic databases

ProtClustDBPRK13300.

Enzyme and pathway databases

BioCycAPER272557:APE1791-MONOMER.

Family and domain databases

HAMAPMF_01264. CCA_arch.
[Tree]
InterProIPR008229. CCA-adding_arc.
IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR015329. tRNA_NucTransf2.
[Graphical view]
KOK07558.
PfamPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFPIRSF005335. CCA_arch. 1 hit.
SUPFAMSSF55003. PAP_C. 1 hit.
TIGRFAMsTIGR03671. Cca_archaeal. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCCA_AERPE
AccessionPrimary (citable) accession number: Q9YB04
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 10, 2007
Last modified: November 16, 2011
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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