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Q9YB01

- TOP1_AERPE

UniProt

Q9YB01 - TOP1_AERPE

Protein

DNA topoisomerase 1

Gene

topA

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi4 – 41Magnesium 1; catalyticUniRule annotation
    Sitei50 – 501Interaction with DNAUniRule annotation
    Metal bindingi103 – 1031Magnesium 1; catalyticUniRule annotation
    Metal bindingi103 – 1031Magnesium 2UniRule annotation
    Metal bindingi105 – 1051Magnesium 2UniRule annotation
    Sitei159 – 1591Interaction with DNAUniRule annotation
    Sitei163 – 1631Interaction with DNAUniRule annotation
    Active sitei311 – 3111O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei313 – 3131Interaction with DNAUniRule annotation
    Sitei501 – 5011Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri595 – 61521C4-typeAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciAPER272557:GJD6-1209-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:APE_1794
    OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
    Taxonomic identifieri272557 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
    ProteomesiUP000002518: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 673673DNA topoisomerase 1PRO_0000145176Add
    BLAST

    Proteomic databases

    PRIDEiQ9YB01.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272557.APE_1794.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9YB01.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 134134ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni189 – 1946Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri595 – 61521C4-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0550.
    HOGENOMiHOG000004019.
    KOiK03168.
    OMAiSNRDFIP.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005739. TopoI_arch.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01057. topA_arch. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9YB01-1 [UniParc]FASTAAdd to Basket

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    MVAEKPKAAA KIAYALSDGR GVLRCSEYGV PYWIVRRDGA AIVVAPSAGH    50
    LFGPHTDSRG FPVFDFEWRP IFEFDRGAGY LSKFYRMLSR ILPGASLYVN 100
    ACDYDIEGSV IGFKIIEAFG DVNRARRMKF STLAPQDIRR AYARMERLDV 150
    EMIEAGMARS EMDWLWGINV SRALMEAARR AAGRRVILSA GRVQSPTLVE 200
    AYRRWREINL HVPKASVAVK ITAEKGGGVF DARPHGWKPQ SLETARSIKS 250
    ELRKNPWLAV EEVRSERSIL RPPPAFNLGD LQKEANRILG LPPLRTQSIA 300
    EELYLEALIS YPRTNSQKLP PSINYRAILD KLAHGPLGRE ARELLKETGG 350
    VLRPVQGSKD DPAHPAIHPT GEKPSQRLSK EHMAVYELIV RRFLAAFSRE 400
    AIVSKSSVLL RDFQGRVWRA EGLRVEDLGW LKYYHYSTPG EKPMPPLDRG 450
    DKARVVRVDV RVEWSQTPVR LDKASLLRWM ESVNIGTEGT RARIIETLYK 500
    RGYLEGSRKS EVTPLGEAVA VIIQTLFPEL SKPDLTRRFE SMIEDIRSGR 550
    RTRQEVIDMS KKTISKLLES FLDRLDTAVR EIGVSLGSVE VEAACHLCGR 600
    KAVSAVSGYR LCSHHMEAFD RLRKALPNLA STISSTPREA LEAIARGRSR 650
    AGAWVRDVAA LALRDDGLYK ALL 673
    Length:673
    Mass (Da):75,571
    Last modified:November 1, 1999 - v1
    Checksum:iD26799337623C584
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA80797.1.
    PIRiH72563.
    RefSeqiNP_148170.1. NC_000854.2.
    WP_010866597.1. NC_000854.2.

    Genome annotation databases

    EnsemblBacteriaiBAA80797; BAA80797; APE_1794.
    GeneIDi1446244.
    KEGGiape:APE_1794.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000002 Genomic DNA. Translation: BAA80797.1 .
    PIRi H72563.
    RefSeqi NP_148170.1. NC_000854.2.
    WP_010866597.1. NC_000854.2.

    3D structure databases

    ProteinModelPortali Q9YB01.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272557.APE_1794.

    Proteomic databases

    PRIDEi Q9YB01.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA80797 ; BAA80797 ; APE_1794 .
    GeneIDi 1446244.
    KEGGi ape:APE_1794.

    Phylogenomic databases

    eggNOGi COG0550.
    HOGENOMi HOG000004019.
    KOi K03168.
    OMAi SNRDFIP.

    Enzyme and pathway databases

    BioCyci APER272557:GJD6-1209-MONOMER.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005739. TopoI_arch.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01057. topA_arch. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

    Entry informationi

    Entry nameiTOP1_AERPE
    AccessioniPrimary (citable) accession number: Q9YB01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3