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Q9YB01

- TOP1_AERPE

UniProt

Q9YB01 - TOP1_AERPE

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Protein

DNA topoisomerase 1

Gene
topA, APE_1794
Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi4 – 41Magnesium 1; catalytic By similarity
Sitei50 – 501Interaction with DNA By similarity
Metal bindingi103 – 1031Magnesium 1; catalytic By similarity
Metal bindingi103 – 1031Magnesium 2 By similarity
Metal bindingi105 – 1051Magnesium 2 By similarity
Sitei159 – 1591Interaction with DNA By similarity
Sitei163 – 1631Interaction with DNA By similarity
Active sitei311 – 3111O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei313 – 3131Interaction with DNA By similarity
Sitei501 – 5011Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri595 – 61521C4-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1209-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:APE_1794
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
ProteomesiUP000002518: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 673673DNA topoisomerase 1UniRule annotationPRO_0000145176Add
BLAST

Proteomic databases

PRIDEiQ9YB01.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272557.APE_1794.

Structurei

3D structure databases

ProteinModelPortaliQ9YB01.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 134134ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1946Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0550.
HOGENOMiHOG000004019.
KOiK03168.
OMAiSNRDFIP.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01057. topA_arch. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9YB01-1 [UniParc]FASTAAdd to Basket

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MVAEKPKAAA KIAYALSDGR GVLRCSEYGV PYWIVRRDGA AIVVAPSAGH    50
LFGPHTDSRG FPVFDFEWRP IFEFDRGAGY LSKFYRMLSR ILPGASLYVN 100
ACDYDIEGSV IGFKIIEAFG DVNRARRMKF STLAPQDIRR AYARMERLDV 150
EMIEAGMARS EMDWLWGINV SRALMEAARR AAGRRVILSA GRVQSPTLVE 200
AYRRWREINL HVPKASVAVK ITAEKGGGVF DARPHGWKPQ SLETARSIKS 250
ELRKNPWLAV EEVRSERSIL RPPPAFNLGD LQKEANRILG LPPLRTQSIA 300
EELYLEALIS YPRTNSQKLP PSINYRAILD KLAHGPLGRE ARELLKETGG 350
VLRPVQGSKD DPAHPAIHPT GEKPSQRLSK EHMAVYELIV RRFLAAFSRE 400
AIVSKSSVLL RDFQGRVWRA EGLRVEDLGW LKYYHYSTPG EKPMPPLDRG 450
DKARVVRVDV RVEWSQTPVR LDKASLLRWM ESVNIGTEGT RARIIETLYK 500
RGYLEGSRKS EVTPLGEAVA VIIQTLFPEL SKPDLTRRFE SMIEDIRSGR 550
RTRQEVIDMS KKTISKLLES FLDRLDTAVR EIGVSLGSVE VEAACHLCGR 600
KAVSAVSGYR LCSHHMEAFD RLRKALPNLA STISSTPREA LEAIARGRSR 650
AGAWVRDVAA LALRDDGLYK ALL 673
Length:673
Mass (Da):75,571
Last modified:November 1, 1999 - v1
Checksum:iD26799337623C584
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000002 Genomic DNA. Translation: BAA80797.1.
PIRiH72563.
RefSeqiNP_148170.1. NC_000854.2.
WP_010866597.1. NC_000854.2.

Genome annotation databases

EnsemblBacteriaiBAA80797; BAA80797; APE_1794.
GeneIDi1446244.
KEGGiape:APE_1794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000002 Genomic DNA. Translation: BAA80797.1 .
PIRi H72563.
RefSeqi NP_148170.1. NC_000854.2.
WP_010866597.1. NC_000854.2.

3D structure databases

ProteinModelPortali Q9YB01.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272557.APE_1794.

Proteomic databases

PRIDEi Q9YB01.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA80797 ; BAA80797 ; APE_1794 .
GeneIDi 1446244.
KEGGi ape:APE_1794.

Phylogenomic databases

eggNOGi COG0550.
HOGENOMi HOG000004019.
KOi K03168.
OMAi SNRDFIP.

Enzyme and pathway databases

BioCyci APER272557:GJD6-1209-MONOMER.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01057. topA_arch. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiTOP1_AERPE
AccessioniPrimary (citable) accession number: Q9YB01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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