Reviewed, UniProtKB/Swiss-Prot Q9YB01 (TOP1_AERPE)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	APE_1794

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 673	673	DNA topoisomerase 1		PRO_0000145176
Regions
	Zinc finger	595 – 615	21	C4-type
Sites
	Active site	311	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YB01-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: D26799337623C584	FASTA	673	75,571
	10 20 30 40 50 60 MVAEKPKAAA KIAYALSDGR GVLRCSEYGV PYWIVRRDGA AIVVAPSAGH LFGPHTDSRG 70 80 90 100 110 120 FPVFDFEWRP IFEFDRGAGY LSKFYRMLSR ILPGASLYVN ACDYDIEGSV IGFKIIEAFG 130 140 150 160 170 180 DVNRARRMKF STLAPQDIRR AYARMERLDV EMIEAGMARS EMDWLWGINV SRALMEAARR 190 200 210 220 230 240 AAGRRVILSA GRVQSPTLVE AYRRWREINL HVPKASVAVK ITAEKGGGVF DARPHGWKPQ 250 260 270 280 290 300 SLETARSIKS ELRKNPWLAV EEVRSERSIL RPPPAFNLGD LQKEANRILG LPPLRTQSIA 310 320 330 340 350 360 EELYLEALIS YPRTNSQKLP PSINYRAILD KLAHGPLGRE ARELLKETGG VLRPVQGSKD 370 380 390 400 410 420 DPAHPAIHPT GEKPSQRLSK EHMAVYELIV RRFLAAFSRE AIVSKSSVLL RDFQGRVWRA 430 440 450 460 470 480 EGLRVEDLGW LKYYHYSTPG EKPMPPLDRG DKARVVRVDV RVEWSQTPVR LDKASLLRWM 490 500 510 520 530 540 ESVNIGTEGT RARIIETLYK RGYLEGSRKS EVTPLGEAVA VIIQTLFPEL SKPDLTRRFE 550 560 570 580 590 600 SMIEDIRSGR RTRQEVIDMS KKTISKLLES FLDRLDTAVR EIGVSLGSVE VEAACHLCGR 610 620 630 640 650 660 KAVSAVSGYR LCSHHMEAFD RLRKALPNLA STISSTPREA LEAIARGRSR AGAWVRDVAA 670 LALRDDGLYK ALL

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA80797.1.
PIR	H72563.
RefSeq	NP_148170.1.
3D structure databases
HSSP	HSSP built from PDB template 1GL9 based on UniProtKB O29238.
ModBase	Search...
Genome annotation databases
GeneID	1446244.
GenomeReviews	Gene locus APE_1794 in contig BA000002_GR.
KEGG	ape:APE_1794.
NMPDR	fig|272557.1.peg.1278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YB01.
OMA	YRERENH.
Enzyme and pathway databases
BRENDA	5.99.1.2. 256344.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR005739. TopoI_arch. IPR006154. Toprim_dom_subgr. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01057. topA_arch. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_AERPE

Accession

Primary (citable) accession number: Q9YB01

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)