Reviewed, UniProtKB/Swiss-Prot Q9YAW0 (KPRS_AERPE)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Ordered Locus Names:	APE_1834.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141233
Regions
	Region	219 – 232	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9YAW0-1 [UniParc]. Last modified July 10, 2007. Version 3. Checksum: 158D7F4A837B31A9	FASTA	309	33,508
	10 20 30 40 50 60 MEGPEQWVIV GGWGSASAEF SEGLSRNMGL KLVKPVFKLF PDEEEYVRIE GDISGFTGAI 70 80 90 100 110 120 VVQSFERPAS RSLVYSLLIA DALKEAGVGR IVLMAPYMGY TRQDRVFLPG EPVSVRAVMR 130 140 150 160 170 180 ALASSGYNAL ASIEVHKEYV LDYFDGSTLN IFPFTYMLKE TGISCGDNTI IVAPDKGSLP 190 200 210 220 230 240 RVERLARETG CRSYGYLVKE RDRITGEVRL AKSTVDPRGK NAIVVDDIIS TGGTIALASQ 250 260 270 280 290 300 WLLENGANSV FVLAAHYLGI GNAEEKMMKA GVSRVVTGNT LPRKPSKIVT YVDLTGLAAG QLTKLVSNL

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA80838.2.
PIR	A72569.
RefSeq	NP_148200.2.
3D structure databases
HSSP	HSSP built from PDB template 1DKU based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	1446274.
GenomeReviews	Gene locus APE_1834.1 in contig BA000002_GR.
KEGG	ape:APE_1834.1.
NMPDR	fig|272557.1.peg.1308.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YAW0.
Enzyme and pathway databases
BRENDA	2.7.6.1. 256344.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_AERPE

Accession

Primary (citable) accession number: Q9YAW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	July 10, 2007
Last modified:	June 16, 2009
This is version 52 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)